Structural Studies of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Tetramer in Complex with Its Receptor, Sialyllactose
The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crys...
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| Veröffentlicht in: | Structure (London) 2005-05, Vol.13 (5), p.803-815 |
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| creator | Yuan, Ping Thompson, Thomas B. Wurzburg, Beth A. Paterson, Reay G. Lamb, Robert A. Jardetzky, Theodore S. |
| description | The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells. |
| doi_str_mv | 10.1016/j.str.2005.02.019 |
| format | Article |
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The structural basis for these diverse functions remains to be fully understood. We report the crystal structures of the parainfluenza virus 5 (SV5) HN and its complexes with sialic acid, the inhibitor DANA, and the receptor sialyllactose. SV5 HN shares common structural features with HN of Newcastle disease virus (NDV) and human parainfluenza 3 (HPIV3), but unlike the previously determined HN structures, the SV5 HN forms a tetramer in solution, which is thought to be the physiological oligomer. The sialyllactose complex reveals intact receptor within the active site, but no major conformational changes in the protein. The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.</description><identifier>ISSN: 0969-2126</identifier><identifier>EISSN: 1878-4186</identifier><identifier>DOI: 10.1016/j.str.2005.02.019</identifier><identifier>PMID: 15893670</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Binding Sites ; Dimerization ; HN Protein - chemistry ; Lactose - analogs & derivatives ; Lactose - chemistry ; Ligands ; Molecular Sequence Data ; N-Acetylneuraminic Acid - chemistry ; Protein Conformation ; Receptors, Virus - chemistry ; Respirovirus - metabolism</subject><ispartof>Structure (London), 2005-05, Vol.13 (5), p.803-815</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-4081ddae932d22434724079e027f1758f26d7a3442104525548eada83211e0253</citedby><cites>FETCH-LOGICAL-c394t-4081ddae932d22434724079e027f1758f26d7a3442104525548eada83211e0253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.str.2005.02.019$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15893670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Ping</creatorcontrib><creatorcontrib>Thompson, Thomas B.</creatorcontrib><creatorcontrib>Wurzburg, Beth A.</creatorcontrib><creatorcontrib>Paterson, Reay G.</creatorcontrib><creatorcontrib>Lamb, Robert A.</creatorcontrib><creatorcontrib>Jardetzky, Theodore S.</creatorcontrib><title>Structural Studies of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Tetramer in Complex with Its Receptor, Sialyllactose</title><title>Structure (London)</title><addtitle>Structure</addtitle><description>The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. 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The SV5 HN structures do not support previously proposed models for HN action in membrane fusion and suggest alternative mechanisms by which HN may promote virus entry into cells.</description><subject>Amino Acid Sequence</subject><subject>Binding Sites</subject><subject>Dimerization</subject><subject>HN Protein - chemistry</subject><subject>Lactose - analogs & derivatives</subject><subject>Lactose - chemistry</subject><subject>Ligands</subject><subject>Molecular Sequence Data</subject><subject>N-Acetylneuraminic Acid - chemistry</subject><subject>Protein Conformation</subject><subject>Receptors, Virus - chemistry</subject><subject>Respirovirus - metabolism</subject><issn>0969-2126</issn><issn>1878-4186</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtuFDEQRS1ERCaBD2CDvGJFN2W33e4WKzQKSaSIICawtYxdnXjUj8EPSFjl03E0I7Fj5bJ07lXVIeQ1g5oBa99v65hCzQFkDbwG1j8jK9aprhKsa5-TFfRtX3HG22NyEuMWALgEeEGOmez6plWwIo-bFLJNOZiRblJ2HiNdBprukH4xwfh5GDPOfwz97kOOVNILnMzt7ZiTn_1cfcaSnMroTER6g6n8MFA_0_Uy7Ua8p799uqOXKdKvaHGXlvCObrwZH8bR2LREfEmOBjNGfHV4T8m3T2c364vq6vr8cv3xqrJNL1IloGPOGewb7jgXjVBcgOoRuBqYkt3AW6dMIwRnICSXUnRonOkazliBZHNK3u57d2H5mTEmPflosawx45KjblXXqFZCAdketGGJMeCgd8FPJjxoBvpJu97qol0_adfAddFeMm8O5fnHhO5f4uC5AB_2AJYTf3kMOlqPs0XnA9qk3eL_U_8Xdp6Tyw</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Yuan, Ping</creator><creator>Thompson, Thomas B.</creator><creator>Wurzburg, Beth A.</creator><creator>Paterson, Reay G.</creator><creator>Lamb, Robert A.</creator><creator>Jardetzky, Theodore S.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Structural Studies of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Tetramer in Complex with Its Receptor, Sialyllactose</title><author>Yuan, Ping ; Thompson, Thomas B. ; Wurzburg, Beth A. ; Paterson, Reay G. ; Lamb, Robert A. ; Jardetzky, Theodore S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-4081ddae932d22434724079e027f1758f26d7a3442104525548eada83211e0253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amino Acid Sequence</topic><topic>Binding Sites</topic><topic>Dimerization</topic><topic>HN Protein - chemistry</topic><topic>Lactose - analogs & derivatives</topic><topic>Lactose - chemistry</topic><topic>Ligands</topic><topic>Molecular Sequence Data</topic><topic>N-Acetylneuraminic Acid - chemistry</topic><topic>Protein Conformation</topic><topic>Receptors, Virus - chemistry</topic><topic>Respirovirus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Ping</creatorcontrib><creatorcontrib>Thompson, Thomas B.</creatorcontrib><creatorcontrib>Wurzburg, Beth A.</creatorcontrib><creatorcontrib>Paterson, Reay G.</creatorcontrib><creatorcontrib>Lamb, Robert A.</creatorcontrib><creatorcontrib>Jardetzky, Theodore S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Structure (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Ping</au><au>Thompson, Thomas B.</au><au>Wurzburg, Beth A.</au><au>Paterson, Reay G.</au><au>Lamb, Robert A.</au><au>Jardetzky, Theodore S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural Studies of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Tetramer in Complex with Its Receptor, Sialyllactose</atitle><jtitle>Structure (London)</jtitle><addtitle>Structure</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>13</volume><issue>5</issue><spage>803</spage><epage>815</epage><pages>803-815</pages><issn>0969-2126</issn><eissn>1878-4186</eissn><abstract>The paramyxovirus hemagglutinin-neuraminidase (HN) functions in virus attachment to cells, cleavage of sialic acid from oligosaccharides, and stimulating membrane fusion during virus entry into cells. 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| source | MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Binding Sites Dimerization HN Protein - chemistry Lactose - analogs & derivatives Lactose - chemistry Ligands Molecular Sequence Data N-Acetylneuraminic Acid - chemistry Protein Conformation Receptors, Virus - chemistry Respirovirus - metabolism |
| title | Structural Studies of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Tetramer in Complex with Its Receptor, Sialyllactose |
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