UV induced bystander signaling leading to apoptosis
Human keratinocytes (HaCaT) were exposed to UV (A+B) (UVA-350–400 mJ/cm 2 and UVB-30 mJ/cm 2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6 h or earlier) did not cause apoptosis in unexposed cells. However, trea...
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| Veröffentlicht in: | Cancer letters 2005-06, Vol.223 (2), p.275-284 |
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| container_title | Cancer letters |
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| creator | Banerjee, Gautam Gupta, Nishma Kapoor, Arun Raman, Govindarajan |
| description | Human keratinocytes (HaCaT) were exposed to UV (A+B) (UVA-350–400
mJ/cm
2 and UVB-30
mJ/cm
2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6
h or earlier) did not cause apoptosis in unexposed cells. However, treatment with conditioned medium collected post UV exposure (1
h) induced Bax in unexposed cells as observed by RT-PCR. The induction of cell death was initiated by conditioned medium collected 12
h after UV exposure and the extent of death was increased progressively when conditioned medium collected 24 or 72
h post UV exposure was used. Medium collected 24
h after UV exposure also increased mitochondrial membrane permeability as determined by rhodamine uptake. Conditioned medium induced apoptosis did not involve reactive oxygen species (ROS) unlike UV induced apoptosis indicating that the apoptosis pathway could be different. Interestingly, at high dilution apototic conditioned medium did not induce apoptosis but actually protected cells from UV insult. The role of nerve growth factor (NGF) in UV induced bystander effects are also discussed. |
| doi_str_mv | 10.1016/j.canlet.2004.09.035 |
| format | Article |
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mJ/cm
2 and UVB-30
mJ/cm
2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6
h or earlier) did not cause apoptosis in unexposed cells. However, treatment with conditioned medium collected post UV exposure (1
h) induced Bax in unexposed cells as observed by RT-PCR. The induction of cell death was initiated by conditioned medium collected 12
h after UV exposure and the extent of death was increased progressively when conditioned medium collected 24 or 72
h post UV exposure was used. Medium collected 24
h after UV exposure also increased mitochondrial membrane permeability as determined by rhodamine uptake. Conditioned medium induced apoptosis did not involve reactive oxygen species (ROS) unlike UV induced apoptosis indicating that the apoptosis pathway could be different. Interestingly, at high dilution apototic conditioned medium did not induce apoptosis but actually protected cells from UV insult. The role of nerve growth factor (NGF) in UV induced bystander effects are also discussed.</description><identifier>ISSN: 0304-3835</identifier><identifier>EISSN: 1872-7980</identifier><identifier>DOI: 10.1016/j.canlet.2004.09.035</identifier><identifier>PMID: 15896462</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Apoptosis ; Bystander Effect ; Cell Membrane Permeability ; Culture Media, Conditioned ; Deoxyribonucleic acid ; DNA ; DNA Damage ; Experiments ; Formazans - pharmacology ; Free radicals ; Gene Expression Regulation ; Humans ; Keratinocytes ; Keratinocytes - physiology ; Mitochondria ; Nerve growth factor (NGF) ; Protection ; Reactive Oxygen Species ; Reverse Transcriptase Polymerase Chain Reaction ; Rodents ; Secreted factors ; Signal Transduction ; Studies ; Tetrazolium Salts - pharmacology ; Ultraviolet Rays ; Up-Regulation</subject><ispartof>Cancer letters, 2005-06, Vol.223 (2), p.275-284</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>Copyright Elsevier Limited Jun 8, 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-98c4ea9f8393c9ed422d6b6c068f8371dd1ce9d084bce11dc00cdf2a5cc9a8fe3</citedby><cites>FETCH-LOGICAL-c388t-98c4ea9f8393c9ed422d6b6c068f8371dd1ce9d084bce11dc00cdf2a5cc9a8fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.canlet.2004.09.035$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15896462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Banerjee, Gautam</creatorcontrib><creatorcontrib>Gupta, Nishma</creatorcontrib><creatorcontrib>Kapoor, Arun</creatorcontrib><creatorcontrib>Raman, Govindarajan</creatorcontrib><title>UV induced bystander signaling leading to apoptosis</title><title>Cancer letters</title><addtitle>Cancer Lett</addtitle><description>Human keratinocytes (HaCaT) were exposed to UV (A+B) (UVA-350–400
mJ/cm
2 and UVB-30
mJ/cm
2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6
h or earlier) did not cause apoptosis in unexposed cells. However, treatment with conditioned medium collected post UV exposure (1
h) induced Bax in unexposed cells as observed by RT-PCR. The induction of cell death was initiated by conditioned medium collected 12
h after UV exposure and the extent of death was increased progressively when conditioned medium collected 24 or 72
h post UV exposure was used. Medium collected 24
h after UV exposure also increased mitochondrial membrane permeability as determined by rhodamine uptake. Conditioned medium induced apoptosis did not involve reactive oxygen species (ROS) unlike UV induced apoptosis indicating that the apoptosis pathway could be different. Interestingly, at high dilution apototic conditioned medium did not induce apoptosis but actually protected cells from UV insult. The role of nerve growth factor (NGF) in UV induced bystander effects are also discussed.</description><subject>Apoptosis</subject><subject>Bystander Effect</subject><subject>Cell Membrane Permeability</subject><subject>Culture Media, Conditioned</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Damage</subject><subject>Experiments</subject><subject>Formazans - pharmacology</subject><subject>Free radicals</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Keratinocytes</subject><subject>Keratinocytes - physiology</subject><subject>Mitochondria</subject><subject>Nerve growth factor (NGF)</subject><subject>Protection</subject><subject>Reactive Oxygen Species</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Rodents</subject><subject>Secreted factors</subject><subject>Signal Transduction</subject><subject>Studies</subject><subject>Tetrazolium Salts - pharmacology</subject><subject>Ultraviolet Rays</subject><subject>Up-Regulation</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6D0QKgrfWSdOP5CLI4hcseHG9hjSZLinddk1aYf-9WbogePA0MDzvO8xDyDWFhAIt7ptEq67FIUkBsgREAiw_IXPKyzQuBYdTMgcGWcw4y2fkwvsGAPKszM_JjOZcFFmRzglbf0a2M6NGE1V7P6jOoIu83XSqtd0malGZwxz6SO363dB76y_JWa1aj1fHuSDr56eP5Wu8en95Wz6uYs04H2LBdYZK1JwJpgWaLE1NURUaCh52JTWGahQGeFZppNRoAG3qVOVaC8VrZAtyN_XuXP81oh_k1nqNbas67EcvizLUpCkE8PYP2PSjCx94SXPIWQlM0EBlE6Vd773DWu6c3Sq3lxTkQals5KRUHpRKEDIoDbGbY_lYbdH8ho4OA_AwARhcfFt00muLXTBqHepBmt7-f-EHojeI_w</recordid><startdate>20050608</startdate><enddate>20050608</enddate><creator>Banerjee, Gautam</creator><creator>Gupta, Nishma</creator><creator>Kapoor, Arun</creator><creator>Raman, Govindarajan</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20050608</creationdate><title>UV induced bystander signaling leading to apoptosis</title><author>Banerjee, Gautam ; Gupta, Nishma ; Kapoor, Arun ; Raman, Govindarajan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-98c4ea9f8393c9ed422d6b6c068f8371dd1ce9d084bce11dc00cdf2a5cc9a8fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Apoptosis</topic><topic>Bystander Effect</topic><topic>Cell Membrane Permeability</topic><topic>Culture Media, Conditioned</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Damage</topic><topic>Experiments</topic><topic>Formazans - pharmacology</topic><topic>Free radicals</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Keratinocytes</topic><topic>Keratinocytes - physiology</topic><topic>Mitochondria</topic><topic>Nerve growth factor (NGF)</topic><topic>Protection</topic><topic>Reactive Oxygen Species</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Rodents</topic><topic>Secreted factors</topic><topic>Signal Transduction</topic><topic>Studies</topic><topic>Tetrazolium Salts - pharmacology</topic><topic>Ultraviolet Rays</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Banerjee, Gautam</creatorcontrib><creatorcontrib>Gupta, Nishma</creatorcontrib><creatorcontrib>Kapoor, Arun</creatorcontrib><creatorcontrib>Raman, Govindarajan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banerjee, Gautam</au><au>Gupta, Nishma</au><au>Kapoor, Arun</au><au>Raman, Govindarajan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UV induced bystander signaling leading to apoptosis</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2005-06-08</date><risdate>2005</risdate><volume>223</volume><issue>2</issue><spage>275</spage><epage>284</epage><pages>275-284</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><abstract>Human keratinocytes (HaCaT) were exposed to UV (A+B) (UVA-350–400
mJ/cm
2 and UVB-30
mJ/cm
2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6
h or earlier) did not cause apoptosis in unexposed cells. However, treatment with conditioned medium collected post UV exposure (1
h) induced Bax in unexposed cells as observed by RT-PCR. The induction of cell death was initiated by conditioned medium collected 12
h after UV exposure and the extent of death was increased progressively when conditioned medium collected 24 or 72
h post UV exposure was used. Medium collected 24
h after UV exposure also increased mitochondrial membrane permeability as determined by rhodamine uptake. Conditioned medium induced apoptosis did not involve reactive oxygen species (ROS) unlike UV induced apoptosis indicating that the apoptosis pathway could be different. Interestingly, at high dilution apototic conditioned medium did not induce apoptosis but actually protected cells from UV insult. The role of nerve growth factor (NGF) in UV induced bystander effects are also discussed.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>15896462</pmid><doi>10.1016/j.canlet.2004.09.035</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cancer letters, 2005-06, Vol.223 (2), p.275-284 |
| issn | 0304-3835 1872-7980 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Apoptosis Bystander Effect Cell Membrane Permeability Culture Media, Conditioned Deoxyribonucleic acid DNA DNA Damage Experiments Formazans - pharmacology Free radicals Gene Expression Regulation Humans Keratinocytes Keratinocytes - physiology Mitochondria Nerve growth factor (NGF) Protection Reactive Oxygen Species Reverse Transcriptase Polymerase Chain Reaction Rodents Secreted factors Signal Transduction Studies Tetrazolium Salts - pharmacology Ultraviolet Rays Up-Regulation |
| title | UV induced bystander signaling leading to apoptosis |
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