Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina
Degeneration of photoreceptors is a consistent and common endpoint in retinal diseases. Herein, we report the efficient induction of photoreceptor-like cells from mouse embryonic stem (ES) cells using chick embryonic retina tissue. Undifferentiated mouse ES cells were initially cultured in a prefere...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2005-06, Vol.332 (1), p.241-247 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 247 |
|---|---|
| container_issue | 1 |
| container_start_page | 241 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 332 |
| creator | Sugie, Yuko Yoshikawa, Masahide Ouji, Yukiteru Saito, Ko Moriya, Kei Ishizaka, Shigeaki Matsuura, Toyoaki Maruoka, Shinji Nawa, Yoshiaki Hara, Yoshiaki |
| description | Degeneration of photoreceptors is a consistent and common endpoint in retinal diseases. Herein, we report the efficient induction of photoreceptor-like cells from mouse embryonic stem (ES) cells using chick embryonic retina tissue. Undifferentiated mouse ES cells were initially cultured in a preferential condition into a neural lineage, and ES cells were then co-cultured with chick embryonic day 6 (E6) retina tissues. After a 10-day co-culture, approximately 20% of the mouse ES derivatives became immuno-positive for rhodopsin. RT-PCR analysis demonstrated expression of the transcription factor crx and a distinct increase of rod photoreceptor-specific markers, IRBP and recoverin, after the start of the co-culture. These results indicate that co-culture of ES cells with chick embryonic retina tissue is a useful and efficient method for the induction of photoreceptor-like cells. |
| doi_str_mv | 10.1016/j.bbrc.2005.04.125 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67835679</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X05008971</els_id><sourcerecordid>20714794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-ba060338f1427564c863255459dc1b2ab494296c0db102950fa52a2d34b3715c3</originalsourceid><addsrcrecordid>eNqFkU1rGzEQhkVJaJykf6CHolNuu53R6sOCXkJIm0CgCUkhN7HSarFcr-VIuwn-95WxobfkNDA88_IyDyFfEWoElN-XtbXJ1QxA1MBrZOITmSFoqBgCPyIzAJAV0_h8Qk5zXgIgcqk_kxMUcy0b1szIw_0ijjF55zdlUOdXq0z7FAc6xCl7ev142NktdbFy02qckqdvYVxQtwjuL_WDTdu4Do4mP4Z1e06O-3aV_ZfDPCN_fl4_Xd1Ud79_3V5d3lWOoxwr24KEppn3yJkSkrt5KSQEF7pzaFlrueZMSwedRWBaQN8K1rKu4bZRKFxzRi72uZsUXyafRzOEvOvarn2pbqSaN0Iq_SHIQCFXmn8IohKKS8UKyPagSzHn5HuzSWFo09YgmJ0aszQ7NWanxgA3RU05-nZIn-zgu_8nBxcF-LEHfPnaa_DJZBf82vkuFD-j6WJ4L_8fEC6d9g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17574672</pqid></control><display><type>article</type><title>Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Sugie, Yuko ; Yoshikawa, Masahide ; Ouji, Yukiteru ; Saito, Ko ; Moriya, Kei ; Ishizaka, Shigeaki ; Matsuura, Toyoaki ; Maruoka, Shinji ; Nawa, Yoshiaki ; Hara, Yoshiaki</creator><creatorcontrib>Sugie, Yuko ; Yoshikawa, Masahide ; Ouji, Yukiteru ; Saito, Ko ; Moriya, Kei ; Ishizaka, Shigeaki ; Matsuura, Toyoaki ; Maruoka, Shinji ; Nawa, Yoshiaki ; Hara, Yoshiaki</creatorcontrib><description>Degeneration of photoreceptors is a consistent and common endpoint in retinal diseases. Herein, we report the efficient induction of photoreceptor-like cells from mouse embryonic stem (ES) cells using chick embryonic retina tissue. Undifferentiated mouse ES cells were initially cultured in a preferential condition into a neural lineage, and ES cells were then co-cultured with chick embryonic day 6 (E6) retina tissues. After a 10-day co-culture, approximately 20% of the mouse ES derivatives became immuno-positive for rhodopsin. RT-PCR analysis demonstrated expression of the transcription factor crx and a distinct increase of rod photoreceptor-specific markers, IRBP and recoverin, after the start of the co-culture. These results indicate that co-culture of ES cells with chick embryonic retina tissue is a useful and efficient method for the induction of photoreceptor-like cells.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.04.125</identifier><identifier>PMID: 15896323</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Differentiation - physiology ; Cells, Cultured ; Chick Embryo ; Chick embryonic retina ; Coculture Techniques - methods ; Differentiation ; ES cells ; Mice ; Photoreceptor cells ; Photoreceptor Cells - cytology ; Retina - cytology ; Retina - embryology ; Stem Cells - cytology ; Tissue Engineering - methods</subject><ispartof>Biochemical and biophysical research communications, 2005-06, Vol.332 (1), p.241-247</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-ba060338f1427564c863255459dc1b2ab494296c0db102950fa52a2d34b3715c3</citedby><cites>FETCH-LOGICAL-c416t-ba060338f1427564c863255459dc1b2ab494296c0db102950fa52a2d34b3715c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2005.04.125$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15896323$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugie, Yuko</creatorcontrib><creatorcontrib>Yoshikawa, Masahide</creatorcontrib><creatorcontrib>Ouji, Yukiteru</creatorcontrib><creatorcontrib>Saito, Ko</creatorcontrib><creatorcontrib>Moriya, Kei</creatorcontrib><creatorcontrib>Ishizaka, Shigeaki</creatorcontrib><creatorcontrib>Matsuura, Toyoaki</creatorcontrib><creatorcontrib>Maruoka, Shinji</creatorcontrib><creatorcontrib>Nawa, Yoshiaki</creatorcontrib><creatorcontrib>Hara, Yoshiaki</creatorcontrib><title>Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Degeneration of photoreceptors is a consistent and common endpoint in retinal diseases. Herein, we report the efficient induction of photoreceptor-like cells from mouse embryonic stem (ES) cells using chick embryonic retina tissue. Undifferentiated mouse ES cells were initially cultured in a preferential condition into a neural lineage, and ES cells were then co-cultured with chick embryonic day 6 (E6) retina tissues. After a 10-day co-culture, approximately 20% of the mouse ES derivatives became immuno-positive for rhodopsin. RT-PCR analysis demonstrated expression of the transcription factor crx and a distinct increase of rod photoreceptor-specific markers, IRBP and recoverin, after the start of the co-culture. These results indicate that co-culture of ES cells with chick embryonic retina tissue is a useful and efficient method for the induction of photoreceptor-like cells.</description><subject>Animals</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Chick Embryo</subject><subject>Chick embryonic retina</subject><subject>Coculture Techniques - methods</subject><subject>Differentiation</subject><subject>ES cells</subject><subject>Mice</subject><subject>Photoreceptor cells</subject><subject>Photoreceptor Cells - cytology</subject><subject>Retina - cytology</subject><subject>Retina - embryology</subject><subject>Stem Cells - cytology</subject><subject>Tissue Engineering - methods</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rGzEQhkVJaJykf6CHolNuu53R6sOCXkJIm0CgCUkhN7HSarFcr-VIuwn-95WxobfkNDA88_IyDyFfEWoElN-XtbXJ1QxA1MBrZOITmSFoqBgCPyIzAJAV0_h8Qk5zXgIgcqk_kxMUcy0b1szIw_0ijjF55zdlUOdXq0z7FAc6xCl7ev142NktdbFy02qckqdvYVxQtwjuL_WDTdu4Do4mP4Z1e06O-3aV_ZfDPCN_fl4_Xd1Ud79_3V5d3lWOoxwr24KEppn3yJkSkrt5KSQEF7pzaFlrueZMSwedRWBaQN8K1rKu4bZRKFxzRi72uZsUXyafRzOEvOvarn2pbqSaN0Iq_SHIQCFXmn8IohKKS8UKyPagSzHn5HuzSWFo09YgmJ0aszQ7NWanxgA3RU05-nZIn-zgu_8nBxcF-LEHfPnaa_DJZBf82vkuFD-j6WJ4L_8fEC6d9g</recordid><startdate>20050624</startdate><enddate>20050624</enddate><creator>Sugie, Yuko</creator><creator>Yoshikawa, Masahide</creator><creator>Ouji, Yukiteru</creator><creator>Saito, Ko</creator><creator>Moriya, Kei</creator><creator>Ishizaka, Shigeaki</creator><creator>Matsuura, Toyoaki</creator><creator>Maruoka, Shinji</creator><creator>Nawa, Yoshiaki</creator><creator>Hara, Yoshiaki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20050624</creationdate><title>Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina</title><author>Sugie, Yuko ; Yoshikawa, Masahide ; Ouji, Yukiteru ; Saito, Ko ; Moriya, Kei ; Ishizaka, Shigeaki ; Matsuura, Toyoaki ; Maruoka, Shinji ; Nawa, Yoshiaki ; Hara, Yoshiaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-ba060338f1427564c863255459dc1b2ab494296c0db102950fa52a2d34b3715c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Chick Embryo</topic><topic>Chick embryonic retina</topic><topic>Coculture Techniques - methods</topic><topic>Differentiation</topic><topic>ES cells</topic><topic>Mice</topic><topic>Photoreceptor cells</topic><topic>Photoreceptor Cells - cytology</topic><topic>Retina - cytology</topic><topic>Retina - embryology</topic><topic>Stem Cells - cytology</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugie, Yuko</creatorcontrib><creatorcontrib>Yoshikawa, Masahide</creatorcontrib><creatorcontrib>Ouji, Yukiteru</creatorcontrib><creatorcontrib>Saito, Ko</creatorcontrib><creatorcontrib>Moriya, Kei</creatorcontrib><creatorcontrib>Ishizaka, Shigeaki</creatorcontrib><creatorcontrib>Matsuura, Toyoaki</creatorcontrib><creatorcontrib>Maruoka, Shinji</creatorcontrib><creatorcontrib>Nawa, Yoshiaki</creatorcontrib><creatorcontrib>Hara, Yoshiaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugie, Yuko</au><au>Yoshikawa, Masahide</au><au>Ouji, Yukiteru</au><au>Saito, Ko</au><au>Moriya, Kei</au><au>Ishizaka, Shigeaki</au><au>Matsuura, Toyoaki</au><au>Maruoka, Shinji</au><au>Nawa, Yoshiaki</au><au>Hara, Yoshiaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-06-24</date><risdate>2005</risdate><volume>332</volume><issue>1</issue><spage>241</spage><epage>247</epage><pages>241-247</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Degeneration of photoreceptors is a consistent and common endpoint in retinal diseases. Herein, we report the efficient induction of photoreceptor-like cells from mouse embryonic stem (ES) cells using chick embryonic retina tissue. Undifferentiated mouse ES cells were initially cultured in a preferential condition into a neural lineage, and ES cells were then co-cultured with chick embryonic day 6 (E6) retina tissues. After a 10-day co-culture, approximately 20% of the mouse ES derivatives became immuno-positive for rhodopsin. RT-PCR analysis demonstrated expression of the transcription factor crx and a distinct increase of rod photoreceptor-specific markers, IRBP and recoverin, after the start of the co-culture. These results indicate that co-culture of ES cells with chick embryonic retina tissue is a useful and efficient method for the induction of photoreceptor-like cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15896323</pmid><doi>10.1016/j.bbrc.2005.04.125</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2005-06, Vol.332 (1), p.241-247 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67835679 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Cell Differentiation - physiology Cells, Cultured Chick Embryo Chick embryonic retina Coculture Techniques - methods Differentiation ES cells Mice Photoreceptor cells Photoreceptor Cells - cytology Retina - cytology Retina - embryology Stem Cells - cytology Tissue Engineering - methods |
| title | Photoreceptor cells from mouse ES cells by co-culture with chick embryonic retina |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T07%3A53%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Photoreceptor%20cells%20from%20mouse%20ES%20cells%20by%20co-culture%20with%20chick%20embryonic%20retina&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Sugie,%20Yuko&rft.date=2005-06-24&rft.volume=332&rft.issue=1&rft.spage=241&rft.epage=247&rft.pages=241-247&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2005.04.125&rft_dat=%3Cproquest_cross%3E20714794%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17574672&rft_id=info:pmid/15896323&rft_els_id=S0006291X05008971&rfr_iscdi=true |