Hypobaric hypoxia affects endogenous levels of α-keto acids in murine heart ventricles

α-Keto acids have recently been identified as potent regulators of cellular adaptations to hypoxia. Their actual intracellular concentrations under such conditions are unknown. Here, we determined concentrations of α-ketobutyrate, α-ketoglutarate, α-ketoisocaproate, α-ketoisovalerate, α-keto-β-methy...

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Veröffentlicht in:	Biochemical and biophysical research communications 2006-04, Vol.342 (3), p.935-939
Hauptverfasser:	Mühling, Jörg, Paddenberg, Renate, Hempelmann, Gunter, Kummer, Wolfgang
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Body Weight Cell Hypoxia - physiology Female Heart Heart Ventricles - metabolism Hypoxia Keto Acids - metabolism Male Mercaptoethanol Mice Phenylenediamines Pyruvate α-Keto acids α-Ketoglutarate
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container_title	Biochemical and biophysical research communications
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creator	Mühling, Jörg Paddenberg, Renate Hempelmann, Gunter Kummer, Wolfgang
description	α-Keto acids have recently been identified as potent regulators of cellular adaptations to hypoxia. Their actual intracellular concentrations under such conditions are unknown. Here, we determined concentrations of α-ketobutyrate, α-ketoglutarate, α-ketoisocaproate, α-ketoisovalerate, α-keto-β-methylvalerate, phenylpyruvate, and pyruvate by a recently developed ultra-sensitive fluorescence HPLC method in ventricular myocardium of mice exposed to hypobaric hypoxia for up to 3 weeks. We observed characteristic alterations of cardiac α-keto acid concentrations that are specific for individual α-keto acids, show significant side differences (right versus left ventricles), and are suited to trigger some of the cardiac metabolic and structural adaptations to chronic hypoxia.
doi_str_mv	10.1016/j.bbrc.2006.02.054
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subjects	Animals Body Weight Cell Hypoxia - physiology Female Heart Heart Ventricles - metabolism Hypoxia Keto Acids - metabolism Male Mercaptoethanol Mice Phenylenediamines Pyruvate α-Keto acids α-Ketoglutarate
title	Hypobaric hypoxia affects endogenous levels of α-keto acids in murine heart ventricles
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