Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro

Telomere shortening is used for replicative aging in primates and ungulates but not rodents. We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae....

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Veröffentlicht in:Mechanisms of ageing and development 2005-06, Vol.126 (6), p.685-691
Hauptverfasser: Forsyth, Nicholas R., Elder, Frederick F.B., Shay, Jerry W., Wright, Woodring E.
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container_end_page 691
container_issue 6
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container_title Mechanisms of ageing and development
container_volume 126
creator Forsyth, Nicholas R.
Elder, Frederick F.B.
Shay, Jerry W.
Wright, Woodring E.
description Telomere shortening is used for replicative aging in primates and ungulates but not rodents. We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae. We examined telomere biology in species representing three leporid genera (European White Rabbit, Black-tailed Jack Rabbit, and Swamp Rabbit) and the monotypic ochotonid genus (North American Pika). Of the leporids one species was a laboratory strain and the others were wild caught. The leporids neither exhibited cellular senescence after sustained periods in culture nor displayed detectable telomerase activity. Continued culture was possible because of their extremely long telomeric arrays. Immunofluorescence showed robust telomere signals at chromosome ends and significant internal chromosomal staining in some instances. Pika was unique in displaying endogenous telomerase activity throughout time in culture. These results show that it is unlikely that lagomorphs use telomere shortening and replicative senescence as a tumor protective mechanism.
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subjects Aging
Animals
Biological and medical sciences
Cells, Cultured
Cellular Senescence - physiology
Development. Metamorphosis. Moult. Ageing
Fundamental and applied biological sciences. Psychology
Hares - physiology
Lagomorpha
Rabbits
Senescence
Telomerase
Telomerase - metabolism
Telomere
Telomere - metabolism
Vertebrates: anatomy and physiology, studies on body, several organs or systems
title Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro
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