Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro
Telomere shortening is used for replicative aging in primates and ungulates but not rodents. We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae....
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Veröffentlicht in: | Mechanisms of ageing and development 2005-06, Vol.126 (6), p.685-691 |
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description | Telomere shortening is used for replicative aging in primates and ungulates but not rodents. We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae. We examined telomere biology in species representing three leporid genera (European White Rabbit, Black-tailed Jack Rabbit, and Swamp Rabbit) and the monotypic ochotonid genus (North American Pika). Of the leporids one species was a laboratory strain and the others were wild caught. The leporids neither exhibited cellular senescence after sustained periods in culture nor displayed detectable telomerase activity. Continued culture was possible because of their extremely long telomeric arrays. Immunofluorescence showed robust telomere signals at chromosome ends and significant internal chromosomal staining in some instances. Pika was unique in displaying endogenous telomerase activity throughout time in culture. These results show that it is unlikely that lagomorphs use telomere shortening and replicative senescence as a tumor protective mechanism. |
doi_str_mv | 10.1016/j.mad.2005.01.003 |
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We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae. We examined telomere biology in species representing three leporid genera (European White Rabbit, Black-tailed Jack Rabbit, and Swamp Rabbit) and the monotypic ochotonid genus (North American Pika). Of the leporids one species was a laboratory strain and the others were wild caught. The leporids neither exhibited cellular senescence after sustained periods in culture nor displayed detectable telomerase activity. Continued culture was possible because of their extremely long telomeric arrays. Immunofluorescence showed robust telomere signals at chromosome ends and significant internal chromosomal staining in some instances. Pika was unique in displaying endogenous telomerase activity throughout time in culture. These results show that it is unlikely that lagomorphs use telomere shortening and replicative senescence as a tumor protective mechanism.</description><subject>Aging</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Cellular Senescence - physiology</subject><subject>Development. Metamorphosis. Moult. Ageing</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hares - physiology</subject><subject>Lagomorpha</subject><subject>Rabbits</subject><subject>Senescence</subject><subject>Telomerase</subject><subject>Telomerase - metabolism</subject><subject>Telomere</subject><subject>Telomere - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0047-6374</issn><issn>1872-6216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90EFrFDEUwPEgit2ufgAvJRfFgjPmJdlkhp6ktCoseNGjhEzyss12ZjImswt-e6fsQm-e3uX3Ho8_Ie-A1cBAfd7Xg_U1Z2xTM6gZEy_IChrNK8VBvSQrxqSulNDyglyWsmeMgeTqNbmATdM0gosV-b21uzSkPD0U-jHbrotz-USn-GgLtaOnDzZjuaY-0THN9FCQztinATNWPmZ0M3qaceqjs3M8IrW7OO5oHOkxzjm9Ia-C7Qu-Pc81-XV_9_P2W7X98fX77Zdt5UQDc9UJlM5x3-rARQcOUQTYtKClBi4dBM9Y8B5a7kMnQUHnVBDaOoC2lVKJNflwujvl9OeAZTZDLA773o6YDsUo3fAGhF4gnKDLqZSMwUw5Djb_NcDMU1OzN0tT89TUMDBL02Xn6nz80A3onzfOERfw_gxscbYP2Y4ulmenGgZCweJuTg6XFMeI2RQXcXR4Kml8iv954x8brZP2</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Forsyth, Nicholas R.</creator><creator>Elder, Frederick F.B.</creator><creator>Shay, Jerry W.</creator><creator>Wright, Woodring E.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro</title><author>Forsyth, Nicholas R. ; Elder, Frederick F.B. ; Shay, Jerry W. ; Wright, Woodring E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-b3e4cc2d97f23b1cee3f1591747124c1fd00fdd192dfb4161bc6f37ac11994463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Cellular Senescence - physiology</topic><topic>Development. Metamorphosis. Moult. Ageing</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hares - physiology</topic><topic>Lagomorpha</topic><topic>Rabbits</topic><topic>Senescence</topic><topic>Telomerase</topic><topic>Telomerase - metabolism</topic><topic>Telomere</topic><topic>Telomere - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Forsyth, Nicholas R.</creatorcontrib><creatorcontrib>Elder, Frederick F.B.</creatorcontrib><creatorcontrib>Shay, Jerry W.</creatorcontrib><creatorcontrib>Wright, Woodring E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of ageing and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Forsyth, Nicholas R.</au><au>Elder, Frederick F.B.</au><au>Shay, Jerry W.</au><au>Wright, Woodring E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro</atitle><jtitle>Mechanisms of ageing and development</jtitle><addtitle>Mech Ageing Dev</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>126</volume><issue>6</issue><spage>685</spage><epage>691</epage><pages>685-691</pages><issn>0047-6374</issn><eissn>1872-6216</eissn><coden>MAGDA3</coden><abstract>Telomere shortening is used for replicative aging in primates and ungulates but not rodents. We examined telomere biology in rabbits to expand the comparative biology of telomere-directed replicative senescence within mammals. The order Lagomorpha consists of two families; Leporidae and Ochotonidae. We examined telomere biology in species representing three leporid genera (European White Rabbit, Black-tailed Jack Rabbit, and Swamp Rabbit) and the monotypic ochotonid genus (North American Pika). Of the leporids one species was a laboratory strain and the others were wild caught. The leporids neither exhibited cellular senescence after sustained periods in culture nor displayed detectable telomerase activity. Continued culture was possible because of their extremely long telomeric arrays. Immunofluorescence showed robust telomere signals at chromosome ends and significant internal chromosomal staining in some instances. Pika was unique in displaying endogenous telomerase activity throughout time in culture. These results show that it is unlikely that lagomorphs use telomere shortening and replicative senescence as a tumor protective mechanism.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15888323</pmid><doi>10.1016/j.mad.2005.01.003</doi><tpages>7</tpages></addata></record> |
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subjects | Aging Animals Biological and medical sciences Cells, Cultured Cellular Senescence - physiology Development. Metamorphosis. Moult. Ageing Fundamental and applied biological sciences. Psychology Hares - physiology Lagomorpha Rabbits Senescence Telomerase Telomerase - metabolism Telomere Telomere - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems |
title | Lagomorphs (rabbits, pikas and hares) do not use telomere-directed replicative aging in vitro |
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