Dye-coupling visualizes networks of large-field motion-sensitive neurons in the fly
In the fly, visually guided course control is accomplished by a set of 60 large-field motion-sensitive neurons in each brain hemisphere. These neurons have been shown to receive retinotopic motion information from local motion detectors on their dendrites. In addition, recent experiments revealed ex...
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| Veröffentlicht in: | Journal of Comparative Physiology 2005-05, Vol.191 (5), p.445-454 |
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| creator | Haag, J Borst, A |
| description | In the fly, visually guided course control is accomplished by a set of 60 large-field motion-sensitive neurons in each brain hemisphere. These neurons have been shown to receive retinotopic motion information from local motion detectors on their dendrites. In addition, recent experiments revealed extensive coupling between the large-field neurons through electrical synapses. These two processes together give rise to their broad and elaborate receptive fields significantly surpassing the extent of their dendritic fields. Here, we demonstrate that the electrical connections between different large-field neurons can be visualized using Neurobiotin dye injection into a single one of them. When combined with a fluorescent dye which does not cross electrical synapses, the injected cell can be identified unambiguously. The Neurobiotin staining corroborates the electrical coupling postulated amongst the cells of the vertical system (VS-cells) and between cells of the horizontal system (HS-cells and CH-cells). In addition, connections between some cells are revealed that have so far not been considered as electrically coupled. |
| doi_str_mv | 10.1007/s00359-005-0605-0 |
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In addition, connections between some cells are revealed that have so far not been considered as electrically coupled.</description><identifier>ISSN: 0340-7594</identifier><identifier>EISSN: 1432-1351</identifier><identifier>DOI: 10.1007/s00359-005-0605-0</identifier><identifier>PMID: 15776269</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Animals ; Axons - physiology ; Biotin - analogs & derivatives ; Biotin - analysis ; brain ; Calliphora vicina ; differential staining ; Diptera ; Diptera - physiology ; Dyes ; Electric Stimulation ; electrical coupling ; electrical synapses ; electrophysiology ; Female ; fluorescein ; Fluorescein - analysis ; fluorescent dyes ; labeling techniques ; Motion detectors ; motion vision ; Movement ; Nerve Net - cytology ; Nerve Net - physiology ; neurobiotin ; Neurons - cytology ; Neurons - physiology ; receptive fields ; sensory neurons ; synapse ; synaptic transmission ; vision</subject><ispartof>Journal of Comparative Physiology, 2005-05, Vol.191 (5), p.445-454</ispartof><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-9492eeb620792342c2af7a4fe6b0cf9254eb2cced56ac4536aea510145d219c43</citedby><cites>FETCH-LOGICAL-c447t-9492eeb620792342c2af7a4fe6b0cf9254eb2cced56ac4536aea510145d219c43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15776269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haag, J</creatorcontrib><creatorcontrib>Borst, A</creatorcontrib><title>Dye-coupling visualizes networks of large-field motion-sensitive neurons in the fly</title><title>Journal of Comparative Physiology</title><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><description>In the fly, visually guided course control is accomplished by a set of 60 large-field motion-sensitive neurons in each brain hemisphere. These neurons have been shown to receive retinotopic motion information from local motion detectors on their dendrites. In addition, recent experiments revealed extensive coupling between the large-field neurons through electrical synapses. These two processes together give rise to their broad and elaborate receptive fields significantly surpassing the extent of their dendritic fields. Here, we demonstrate that the electrical connections between different large-field neurons can be visualized using Neurobiotin dye injection into a single one of them. When combined with a fluorescent dye which does not cross electrical synapses, the injected cell can be identified unambiguously. The Neurobiotin staining corroborates the electrical coupling postulated amongst the cells of the vertical system (VS-cells) and between cells of the horizontal system (HS-cells and CH-cells). In addition, connections between some cells are revealed that have so far not been considered as electrically coupled.</description><subject>Animals</subject><subject>Axons - physiology</subject><subject>Biotin - analogs & derivatives</subject><subject>Biotin - analysis</subject><subject>brain</subject><subject>Calliphora vicina</subject><subject>differential staining</subject><subject>Diptera</subject><subject>Diptera - physiology</subject><subject>Dyes</subject><subject>Electric Stimulation</subject><subject>electrical coupling</subject><subject>electrical synapses</subject><subject>electrophysiology</subject><subject>Female</subject><subject>fluorescein</subject><subject>Fluorescein - analysis</subject><subject>fluorescent dyes</subject><subject>labeling techniques</subject><subject>Motion detectors</subject><subject>motion vision</subject><subject>Movement</subject><subject>Nerve Net - cytology</subject><subject>Nerve Net - physiology</subject><subject>neurobiotin</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>receptive fields</subject><subject>sensory neurons</subject><subject>synapse</subject><subject>synaptic transmission</subject><subject>vision</subject><issn>0340-7594</issn><issn>1432-1351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1vEzEURS1ERUPhB7CBEQt2Lu_5c2aJSluQKnVRurYc5zm4TMbBnilKfz0TJVIlNmze25x7pavD2DuEcwSwnyuA1B0H0BzM_rxgC1RScJQaX7IFSAXc6k6dste1PgCAQIGv2Clqa40w3YLdfd0RD3na9mlYN4-pTr5PT1SbgcY_ufyqTY5N78uaeEzUr5pNHlMeeKWhpjE90gxOJQ-1SUMz_qQm9rs37CT6vtLb4z9j91eXPy6-8Zvb6-8XX254UMqOvFOdIFoaAbYTUokgfLReRTJLCLETWtFShEArbXxQWhpPXiOg0iuBXVDyjH069G5L_j1RHd0m1UB97wfKU3XGtqIFbf4LopVtCwpn8OM_4EOeyjCPcAalMa2w7QzhAQol11ooum1JG192DsHtvbiDFzd7cXsvDubM-2PxtNzQ6jlxFDEDHw5A9Nn5dUnV3d8JQAkIYl4h5F_PxJFo</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Haag, J</creator><creator>Borst, A</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Dye-coupling visualizes networks of large-field motion-sensitive neurons in the fly</title><author>Haag, J ; Borst, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-9492eeb620792342c2af7a4fe6b0cf9254eb2cced56ac4536aea510145d219c43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Axons - physiology</topic><topic>Biotin - analogs & derivatives</topic><topic>Biotin - analysis</topic><topic>brain</topic><topic>Calliphora vicina</topic><topic>differential staining</topic><topic>Diptera</topic><topic>Diptera - physiology</topic><topic>Dyes</topic><topic>Electric Stimulation</topic><topic>electrical coupling</topic><topic>electrical synapses</topic><topic>electrophysiology</topic><topic>Female</topic><topic>fluorescein</topic><topic>Fluorescein - analysis</topic><topic>fluorescent dyes</topic><topic>labeling techniques</topic><topic>Motion detectors</topic><topic>motion vision</topic><topic>Movement</topic><topic>Nerve Net - cytology</topic><topic>Nerve Net - physiology</topic><topic>neurobiotin</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>receptive fields</topic><topic>sensory neurons</topic><topic>synapse</topic><topic>synaptic transmission</topic><topic>vision</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haag, J</creatorcontrib><creatorcontrib>Borst, A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Comparative Physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haag, J</au><au>Borst, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dye-coupling visualizes networks of large-field motion-sensitive neurons in the fly</atitle><jtitle>Journal of Comparative Physiology</jtitle><addtitle>J Comp Physiol A Neuroethol Sens Neural Behav Physiol</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>191</volume><issue>5</issue><spage>445</spage><epage>454</epage><pages>445-454</pages><issn>0340-7594</issn><eissn>1432-1351</eissn><abstract>In the fly, visually guided course control is accomplished by a set of 60 large-field motion-sensitive neurons in each brain hemisphere. 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| subjects | Animals Axons - physiology Biotin - analogs & derivatives Biotin - analysis brain Calliphora vicina differential staining Diptera Diptera - physiology Dyes Electric Stimulation electrical coupling electrical synapses electrophysiology Female fluorescein Fluorescein - analysis fluorescent dyes labeling techniques Motion detectors motion vision Movement Nerve Net - cytology Nerve Net - physiology neurobiotin Neurons - cytology Neurons - physiology receptive fields sensory neurons synapse synaptic transmission vision |
| title | Dye-coupling visualizes networks of large-field motion-sensitive neurons in the fly |
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