Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons
A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons an...
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| Veröffentlicht in: | Veterinary record 2006-04, Vol.158 (13), p.442-444 |
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| creator | Arca-Ruibal, B. Wernery, U. Zachariah, R. Bailey, T. A. Di Somma, A. Silvanose, C. McKinney, P. |
| description | A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 per cent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons. |
| doi_str_mv | 10.1136/vr.158.13.442 |
| format | Article |
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A. ; Di Somma, A. ; Silvanose, C. ; McKinney, P.</creator><creatorcontrib>Arca-Ruibal, B. ; Wernery, U. ; Zachariah, R. ; Bailey, T. A. ; Di Somma, A. ; Silvanose, C. ; McKinney, P.</creatorcontrib><description>A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 per cent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons.</description><identifier>ISSN: 0042-4900</identifier><identifier>EISSN: 2042-7670</identifier><identifier>DOI: 10.1136/vr.158.13.442</identifier><identifier>PMID: 16581995</identifier><language>eng</language><publisher>England: BMJ Publishing Group Limited</publisher><subject>Animals ; Antigens, Fungal - blood ; aspergillosis ; Aspergillosis - blood ; Aspergillosis - diagnosis ; Aspergillosis - veterinary ; Aspergillus - immunology ; Aspergillus - isolation & purification ; Bird Diseases - blood ; Bird Diseases - diagnosis ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - veterinary ; Falconiformes ; falcons ; Mannans - blood ; Sensitivity and Specificity</subject><ispartof>Veterinary record, 2006-04, Vol.158 (13), p.442-444</ispartof><rights>British Veterinary Association. All rights reserved.</rights><rights>British Veterinary Association 2006</rights><rights>Copyright: 2006 British Veterinary Association. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b4230-5830e486bcb6f63dd182e05ce33f32229a00cc9bc301417a58766e072ceebcfb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1136%2Fvr.158.13.442$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1136%2Fvr.158.13.442$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16581995$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arca-Ruibal, B.</creatorcontrib><creatorcontrib>Wernery, U.</creatorcontrib><creatorcontrib>Zachariah, R.</creatorcontrib><creatorcontrib>Bailey, T. A.</creatorcontrib><creatorcontrib>Di Somma, A.</creatorcontrib><creatorcontrib>Silvanose, C.</creatorcontrib><creatorcontrib>McKinney, P.</creatorcontrib><title>Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons</title><title>Veterinary record</title><addtitle>Vet Rec</addtitle><description>A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 per cent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons.</description><subject>Animals</subject><subject>Antigens, Fungal - blood</subject><subject>aspergillosis</subject><subject>Aspergillosis - blood</subject><subject>Aspergillosis - diagnosis</subject><subject>Aspergillosis - veterinary</subject><subject>Aspergillus - immunology</subject><subject>Aspergillus - isolation & purification</subject><subject>Bird Diseases - blood</subject><subject>Bird Diseases - diagnosis</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Falconiformes</subject><subject>falcons</subject><subject>Mannans - blood</subject><subject>Sensitivity and Specificity</subject><issn>0042-4900</issn><issn>2042-7670</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqF0M1r2zAYBnAxNtas23HXzTAYvTh79WFJPqYl7QqBwfpxFbL8OlWwrUxKWvrfT6kDgx22k17E732QHkI-UphTyuW3xzinlZ5TPheCvSIzBoKVSip4TWZwmEUNcELepbQBYHXF2VtyQmWlaV1XM3K_SAlTGnDcFaErbOHCMGB03vZFsmP75N1DsVxd3ywKPxa7Byxab9djSD69-LTFuPZ9_3KRRWd7F8b0nrzJU8IPx_OU3F0uby--l6sfV9cXi1XZCMahrDQHFFo2rpGd5G1LNUOoHHLeccZYbQGcqxvHgQqqbKWVlAiKOcTGdQ0_JV-n3G0Mv_aYdmbwyWHf2xHDPhmpNKMVExl--Qtuwj6O-W2GKlULrQFUVuWkXAwpRezMNvrBxmdDwRzqNo_R5LoN5SbXnf2nY-q-GbD9o4_9ZiAn8OR7fP53mrlf3v48v8y_45AXP0-LnQ3GrqNP5u6GAc1FZMD4IfpsEs2w-c8zfwOKqqAj</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Arca-Ruibal, B.</creator><creator>Wernery, U.</creator><creator>Zachariah, R.</creator><creator>Bailey, T. 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A. ; Di Somma, A. ; Silvanose, C. ; McKinney, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b4230-5830e486bcb6f63dd182e05ce33f32229a00cc9bc301417a58766e072ceebcfb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens, Fungal - blood</topic><topic>aspergillosis</topic><topic>Aspergillosis - blood</topic><topic>Aspergillosis - diagnosis</topic><topic>Aspergillosis - veterinary</topic><topic>Aspergillus - immunology</topic><topic>Aspergillus - isolation & purification</topic><topic>Bird Diseases - blood</topic><topic>Bird Diseases - diagnosis</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Falconiformes</topic><topic>falcons</topic><topic>Mannans - blood</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arca-Ruibal, B.</creatorcontrib><creatorcontrib>Wernery, U.</creatorcontrib><creatorcontrib>Zachariah, R.</creatorcontrib><creatorcontrib>Bailey, T. 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A.</au><au>Di Somma, A.</au><au>Silvanose, C.</au><au>McKinney, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons</atitle><jtitle>Veterinary record</jtitle><addtitle>Vet Rec</addtitle><date>2006-04</date><risdate>2006</risdate><volume>158</volume><issue>13</issue><spage>442</spage><epage>444</epage><pages>442-444</pages><issn>0042-4900</issn><eissn>2042-7670</eissn><abstract>A commercial sandwich ELISA (Platelia Aspergillus EIA; Bio-Rad) developed for the detection of galactomannan, a major cell wall constituent of Aspergillus species, was tested for its efficacy in the diagnosis of aspergillosis in falcons. Ninety serum samples from 50 aspergillosis-positive falcons and 182 samples from 142 aspergillosis-negative falcons were tested. The sensitivity of the test was only 12 per cent and its specificity was 95 per cent. The test was therefore unsatisfactory for detecting galactomannan in the serum samples and cannot be used as a screening test for aspergillosis in falcons.</abstract><cop>England</cop><pub>BMJ Publishing Group Limited</pub><pmid>16581995</pmid><doi>10.1136/vr.158.13.442</doi><tpages>3</tpages></addata></record> |
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| ispartof | Veterinary record, 2006-04, Vol.158 (13), p.442-444 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antigens, Fungal - blood aspergillosis Aspergillosis - blood Aspergillosis - diagnosis Aspergillosis - veterinary Aspergillus - immunology Aspergillus - isolation & purification Bird Diseases - blood Bird Diseases - diagnosis enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary Falconiformes falcons Mannans - blood Sensitivity and Specificity |
| title | Assessment of a commercial sandwich ELISA in the diagnosis of aspergillosis in falcons |
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