Pyruvate but not lactate prevents NADH-induced myoglobin oxidation

In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of...

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Veröffentlicht in:Free radical biology & medicine 2005-06, Vol.38 (11), p.1484-1490
Hauptverfasser: Olek, Robert A., Antosiewicz, Jedrzej, Popinigis, Jerzy, Gabbianelli, Rosita, Fedeli, Donatella, Falcioni, Giancarlo
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container_end_page 1490
container_issue 11
container_start_page 1484
container_title Free radical biology & medicine
container_volume 38
creator Olek, Robert A.
Antosiewicz, Jedrzej
Popinigis, Jerzy
Gabbianelli, Rosita
Fedeli, Donatella
Falcioni, Giancarlo
description In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by l-lactate. The antioxidant activity versus H 2O 2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia–reperfusion injury during infarction or high–intensity exercise.
doi_str_mv 10.1016/j.freeradbiomed.2005.02.018
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Animals
Antioxidants - metabolism
Antioxidants - pharmacology
Catalase
Catalase - metabolism
Chemiluminescence
Dose-Response Relationship, Drug
Free radicals
Free Radicals - metabolism
Horses
Hydrogen Peroxide - pharmacology
Hydrogen-Ion Concentration
Kinetics
l-lactate
Lactates - metabolism
Lactic Acid - chemistry
Lactic Acid - metabolism
Luminol
Myocardium - metabolism
Myoglobin
Myoglobin - chemistry
Myoglobin - metabolism
NAD - chemistry
NAD - metabolism
NADH
Oxidation-Reduction
Oxygen - metabolism
Pyruvate
Pyruvic Acid - chemistry
Pyruvic Acid - metabolism
Reperfusion Injury
ROS
Time Factors
title Pyruvate but not lactate prevents NADH-induced myoglobin oxidation
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