Pyruvate but not lactate prevents NADH-induced myoglobin oxidation
In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of...
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creator | Olek, Robert A. Antosiewicz, Jedrzej Popinigis, Jerzy Gabbianelli, Rosita Fedeli, Donatella Falcioni, Giancarlo |
description | In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by
l-lactate. The antioxidant activity versus H
2O
2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia–reperfusion injury during infarction or high–intensity exercise. |
doi_str_mv | 10.1016/j.freeradbiomed.2005.02.018 |
format | Article |
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l-lactate. The antioxidant activity versus H
2O
2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia–reperfusion injury during infarction or high–intensity exercise.</description><identifier>ISSN: 0891-5849</identifier><identifier>EISSN: 1873-4596</identifier><identifier>DOI: 10.1016/j.freeradbiomed.2005.02.018</identifier><identifier>PMID: 15890622</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Antioxidants - metabolism ; Antioxidants - pharmacology ; Catalase ; Catalase - metabolism ; Chemiluminescence ; Dose-Response Relationship, Drug ; Free radicals ; Free Radicals - metabolism ; Horses ; Hydrogen Peroxide - pharmacology ; Hydrogen-Ion Concentration ; Kinetics ; l-lactate ; Lactates - metabolism ; Lactic Acid - chemistry ; Lactic Acid - metabolism ; Luminol ; Myocardium - metabolism ; Myoglobin ; Myoglobin - chemistry ; Myoglobin - metabolism ; NAD - chemistry ; NAD - metabolism ; NADH ; Oxidation-Reduction ; Oxygen - metabolism ; Pyruvate ; Pyruvic Acid - chemistry ; Pyruvic Acid - metabolism ; Reperfusion Injury ; ROS ; Time Factors</subject><ispartof>Free radical biology & medicine, 2005-06, Vol.38 (11), p.1484-1490</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-cb206bd0f16ee1338a7cdd15a47762a163315c1891c28444824a60231279edf93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.freeradbiomed.2005.02.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15890622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Olek, Robert A.</creatorcontrib><creatorcontrib>Antosiewicz, Jedrzej</creatorcontrib><creatorcontrib>Popinigis, Jerzy</creatorcontrib><creatorcontrib>Gabbianelli, Rosita</creatorcontrib><creatorcontrib>Fedeli, Donatella</creatorcontrib><creatorcontrib>Falcioni, Giancarlo</creatorcontrib><title>Pyruvate but not lactate prevents NADH-induced myoglobin oxidation</title><title>Free radical biology & medicine</title><addtitle>Free Radic Biol Med</addtitle><description>In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by
l-lactate. The antioxidant activity versus H
2O
2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia–reperfusion injury during infarction or high–intensity exercise.</description><subject>Animals</subject><subject>Antioxidants - metabolism</subject><subject>Antioxidants - pharmacology</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>Chemiluminescence</subject><subject>Dose-Response Relationship, Drug</subject><subject>Free radicals</subject><subject>Free Radicals - metabolism</subject><subject>Horses</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>l-lactate</subject><subject>Lactates - metabolism</subject><subject>Lactic Acid - chemistry</subject><subject>Lactic Acid - metabolism</subject><subject>Luminol</subject><subject>Myocardium - metabolism</subject><subject>Myoglobin</subject><subject>Myoglobin - chemistry</subject><subject>Myoglobin - metabolism</subject><subject>NAD - chemistry</subject><subject>NAD - metabolism</subject><subject>NADH</subject><subject>Oxidation-Reduction</subject><subject>Oxygen - metabolism</subject><subject>Pyruvate</subject><subject>Pyruvic Acid - chemistry</subject><subject>Pyruvic Acid - metabolism</subject><subject>Reperfusion Injury</subject><subject>ROS</subject><subject>Time Factors</subject><issn>0891-5849</issn><issn>1873-4596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1r3DAQhkVp6G7S_IViKPRmZ_RhWaanbZovCEkOzVnI0rho8VpbSV6y_75edin0ltMwzPvOO_MQ8pVCRYHKq3XVR8RoXOfDBl3FAOoKWAVUfSBLqhpeirqVH8kSVEvLWol2Qc5TWgOAqLn6RBa0Vi1Ixpbkx8s-TjuTseimXIwhF4Ox-dBvI-5wzKl4Wv28L_3oJouu2OzD7yF0fizCm3cm-zB-Jme9GRJenuoFeb29-XV9Xz4-3z1crx5LyxXNpe0YyM5BTyUi5VyZxjpHayOaRjJDJee0tnQ-2TIlhFBMGAmMU9a06PqWX5Bvx73bGP5MmLLe-GRxGMyIYUpaNopBLdgs_H4U2hhSitjrbfQbE_eagj4g1Gv9H0J9QKiB6Rnh7P5yipm6w-yf98RsFtwcBTg_u_MYdbIex5mOj2izdsG_K-gv8L2JOg</recordid><startdate>20050601</startdate><enddate>20050601</enddate><creator>Olek, Robert A.</creator><creator>Antosiewicz, Jedrzej</creator><creator>Popinigis, Jerzy</creator><creator>Gabbianelli, Rosita</creator><creator>Fedeli, Donatella</creator><creator>Falcioni, Giancarlo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050601</creationdate><title>Pyruvate but not lactate prevents NADH-induced myoglobin oxidation</title><author>Olek, Robert A. ; Antosiewicz, Jedrzej ; Popinigis, Jerzy ; Gabbianelli, Rosita ; Fedeli, Donatella ; Falcioni, Giancarlo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-cb206bd0f16ee1338a7cdd15a47762a163315c1891c28444824a60231279edf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Antioxidants - metabolism</topic><topic>Antioxidants - pharmacology</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>Chemiluminescence</topic><topic>Dose-Response Relationship, Drug</topic><topic>Free radicals</topic><topic>Free Radicals - metabolism</topic><topic>Horses</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>l-lactate</topic><topic>Lactates - metabolism</topic><topic>Lactic Acid - chemistry</topic><topic>Lactic Acid - metabolism</topic><topic>Luminol</topic><topic>Myocardium - metabolism</topic><topic>Myoglobin</topic><topic>Myoglobin - chemistry</topic><topic>Myoglobin - metabolism</topic><topic>NAD - chemistry</topic><topic>NAD - metabolism</topic><topic>NADH</topic><topic>Oxidation-Reduction</topic><topic>Oxygen - metabolism</topic><topic>Pyruvate</topic><topic>Pyruvic Acid - chemistry</topic><topic>Pyruvic Acid - metabolism</topic><topic>Reperfusion Injury</topic><topic>ROS</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olek, Robert A.</creatorcontrib><creatorcontrib>Antosiewicz, Jedrzej</creatorcontrib><creatorcontrib>Popinigis, Jerzy</creatorcontrib><creatorcontrib>Gabbianelli, Rosita</creatorcontrib><creatorcontrib>Fedeli, Donatella</creatorcontrib><creatorcontrib>Falcioni, Giancarlo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Free radical biology & medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olek, Robert A.</au><au>Antosiewicz, Jedrzej</au><au>Popinigis, Jerzy</au><au>Gabbianelli, Rosita</au><au>Fedeli, Donatella</au><au>Falcioni, Giancarlo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pyruvate but not lactate prevents NADH-induced myoglobin oxidation</atitle><jtitle>Free radical biology & medicine</jtitle><addtitle>Free Radic Biol Med</addtitle><date>2005-06-01</date><risdate>2005</risdate><volume>38</volume><issue>11</issue><spage>1484</spage><epage>1490</epage><pages>1484-1490</pages><issn>0891-5849</issn><eissn>1873-4596</eissn><abstract>In this work, we investigated the influence of NADH on the redox state of myoglobin and the roles of pyruvate and lactate in this process. NADH increased the autoxidation rate of myoglobin. Both a drop in pH and partial deoxygenation markedly stimulated the autoxidation process and the influence of NADH. A correlation between met-Mb formation rate and NADH oxidation rate was always observed. The increased rate of Mb autoxidation caused by NADH was inhibited by catalase and pyruvate but not by
l-lactate. The antioxidant activity versus H
2O
2 of both pyruvate and lactate was evidenced by chemiluminescence experiments. The antioxidant activity of lactate disappeared completely in the presence of myoglobin or apo-myoglobin, whereas it was only reduced for pyruvate. These results could be of interest in preventing autoxidation of myoglobin that can contribute to ischemia–reperfusion injury during infarction or high–intensity exercise.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15890622</pmid><doi>10.1016/j.freeradbiomed.2005.02.018</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Antioxidants - metabolism Antioxidants - pharmacology Catalase Catalase - metabolism Chemiluminescence Dose-Response Relationship, Drug Free radicals Free Radicals - metabolism Horses Hydrogen Peroxide - pharmacology Hydrogen-Ion Concentration Kinetics l-lactate Lactates - metabolism Lactic Acid - chemistry Lactic Acid - metabolism Luminol Myocardium - metabolism Myoglobin Myoglobin - chemistry Myoglobin - metabolism NAD - chemistry NAD - metabolism NADH Oxidation-Reduction Oxygen - metabolism Pyruvate Pyruvic Acid - chemistry Pyruvic Acid - metabolism Reperfusion Injury ROS Time Factors |
title | Pyruvate but not lactate prevents NADH-induced myoglobin oxidation |
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