Microdevice DNA forensics by the simple tandem repeat method
We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Ful...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2006-04, Vol.1111 (2), p.206-213 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 213 |
|---|---|
| container_issue | 2 |
| container_start_page | 206 |
| container_title | Journal of Chromatography A |
| container_volume | 1111 |
| creator | Goedecke, Nils McKenna, Brian El-Difrawy, Sameh Gismondi, Elizabeth Swenson, Abigail Carey, Loucinda Matsudaira, Paul Ehrlich, Daniel J. |
| description | We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures. |
| doi_str_mv | 10.1016/j.chroma.2005.05.100 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67813717</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967305010733</els_id><sourcerecordid>67813717</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-680c75f686ace90abe3ba20a51b35ab74e9f5ffe39c702d954083be99c53caac3</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhbNQbK3-A5Gs3M14M2mSCYhQfEPVja5DJnOHpszLZFrov3dKC-6EA3dzzrmcj5ArBikDJm_XqVuFrrFpBiDSUQzghEwBMpZoqfiEnMe4BmAKVHZGJkwKqYXSU3L37l3oStx6h_TxY0GrLmAbvYu02NFhhTT6pq-RDrYtsaEBe7QDbXBYdeUFOa1sHfHyeGfk-_np6-E1WX6-vD0slonjEoZE5uCUqGQurUMNtkBe2AysYAUXtlBz1JWoKuTaKchKLeaQ8wK1doI7ax2fkZtDbx-6nw3GwTQ-Oqxr22K3iUaqnHHF1GicH4zjphgDVqYPvrFhZxiYPSmzNgdSZk_KjBpJjbHrY_-maLD8Cx0xjYb7gwHHlVuPwUTnsXVY-oBuMGXn___wCyZ5fcY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67813717</pqid></control><display><type>article</type><title>Microdevice DNA forensics by the simple tandem repeat method</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Goedecke, Nils ; McKenna, Brian ; El-Difrawy, Sameh ; Gismondi, Elizabeth ; Swenson, Abigail ; Carey, Loucinda ; Matsudaira, Paul ; Ehrlich, Daniel J.</creator><creatorcontrib>Goedecke, Nils ; McKenna, Brian ; El-Difrawy, Sameh ; Gismondi, Elizabeth ; Swenson, Abigail ; Carey, Loucinda ; Matsudaira, Paul ; Ehrlich, Daniel J.</creatorcontrib><description>We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2005.05.100</identifier><identifier>PMID: 16569579</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>DNA - genetics ; Forensic DNA analysis ; Forensic Medicine ; Humans ; Microdevice electrophoresis ; Microfluidics ; Short tandem repeat ; Tandem Repeat Sequences</subject><ispartof>Journal of Chromatography A, 2006-04, Vol.1111 (2), p.206-213</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-680c75f686ace90abe3ba20a51b35ab74e9f5ffe39c702d954083be99c53caac3</citedby><cites>FETCH-LOGICAL-c360t-680c75f686ace90abe3ba20a51b35ab74e9f5ffe39c702d954083be99c53caac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0021967305010733$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16569579$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goedecke, Nils</creatorcontrib><creatorcontrib>McKenna, Brian</creatorcontrib><creatorcontrib>El-Difrawy, Sameh</creatorcontrib><creatorcontrib>Gismondi, Elizabeth</creatorcontrib><creatorcontrib>Swenson, Abigail</creatorcontrib><creatorcontrib>Carey, Loucinda</creatorcontrib><creatorcontrib>Matsudaira, Paul</creatorcontrib><creatorcontrib>Ehrlich, Daniel J.</creatorcontrib><title>Microdevice DNA forensics by the simple tandem repeat method</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.</description><subject>DNA - genetics</subject><subject>Forensic DNA analysis</subject><subject>Forensic Medicine</subject><subject>Humans</subject><subject>Microdevice electrophoresis</subject><subject>Microfluidics</subject><subject>Short tandem repeat</subject><subject>Tandem Repeat Sequences</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhbNQbK3-A5Gs3M14M2mSCYhQfEPVja5DJnOHpszLZFrov3dKC-6EA3dzzrmcj5ArBikDJm_XqVuFrrFpBiDSUQzghEwBMpZoqfiEnMe4BmAKVHZGJkwKqYXSU3L37l3oStx6h_TxY0GrLmAbvYu02NFhhTT6pq-RDrYtsaEBe7QDbXBYdeUFOa1sHfHyeGfk-_np6-E1WX6-vD0slonjEoZE5uCUqGQurUMNtkBe2AysYAUXtlBz1JWoKuTaKchKLeaQ8wK1doI7ax2fkZtDbx-6nw3GwTQ-Oqxr22K3iUaqnHHF1GicH4zjphgDVqYPvrFhZxiYPSmzNgdSZk_KjBpJjbHrY_-maLD8Cx0xjYb7gwHHlVuPwUTnsXVY-oBuMGXn___wCyZ5fcY</recordid><startdate>20060414</startdate><enddate>20060414</enddate><creator>Goedecke, Nils</creator><creator>McKenna, Brian</creator><creator>El-Difrawy, Sameh</creator><creator>Gismondi, Elizabeth</creator><creator>Swenson, Abigail</creator><creator>Carey, Loucinda</creator><creator>Matsudaira, Paul</creator><creator>Ehrlich, Daniel J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060414</creationdate><title>Microdevice DNA forensics by the simple tandem repeat method</title><author>Goedecke, Nils ; McKenna, Brian ; El-Difrawy, Sameh ; Gismondi, Elizabeth ; Swenson, Abigail ; Carey, Loucinda ; Matsudaira, Paul ; Ehrlich, Daniel J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-680c75f686ace90abe3ba20a51b35ab74e9f5ffe39c702d954083be99c53caac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>DNA - genetics</topic><topic>Forensic DNA analysis</topic><topic>Forensic Medicine</topic><topic>Humans</topic><topic>Microdevice electrophoresis</topic><topic>Microfluidics</topic><topic>Short tandem repeat</topic><topic>Tandem Repeat Sequences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goedecke, Nils</creatorcontrib><creatorcontrib>McKenna, Brian</creatorcontrib><creatorcontrib>El-Difrawy, Sameh</creatorcontrib><creatorcontrib>Gismondi, Elizabeth</creatorcontrib><creatorcontrib>Swenson, Abigail</creatorcontrib><creatorcontrib>Carey, Loucinda</creatorcontrib><creatorcontrib>Matsudaira, Paul</creatorcontrib><creatorcontrib>Ehrlich, Daniel J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goedecke, Nils</au><au>McKenna, Brian</au><au>El-Difrawy, Sameh</au><au>Gismondi, Elizabeth</au><au>Swenson, Abigail</au><au>Carey, Loucinda</au><au>Matsudaira, Paul</au><au>Ehrlich, Daniel J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microdevice DNA forensics by the simple tandem repeat method</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2006-04-14</date><risdate>2006</risdate><volume>1111</volume><issue>2</issue><spage>206</spage><epage>213</epage><pages>206-213</pages><issn>0021-9673</issn><abstract>We review recent experiments on DNA forensics by the simple tandem repeat (STR) method using a 16-lane micromachined device as the active separation element. Separations by linear polyacrylamide matrices show very high data quality metrics when evaluated with statistically significant data sets. Full 16-locus multiplexes are verified on the multilane system. Multi-donor mixed samples are studied in the context of the limits of the laser-induced fluorescence detector and data-reduction software. The microdevice appears to be posed to outperform current capillary arrays in terms of stability and, through specialized sample loading, in the interpretation of complex mixtures.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>16569579</pmid><doi>10.1016/j.chroma.2005.05.100</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2006-04, Vol.1111 (2), p.206-213 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67813717 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | DNA - genetics Forensic DNA analysis Forensic Medicine Humans Microdevice electrophoresis Microfluidics Short tandem repeat Tandem Repeat Sequences |
| title | Microdevice DNA forensics by the simple tandem repeat method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T15%3A19%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Microdevice%20DNA%20forensics%20by%20the%20simple%20tandem%20repeat%20method&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Goedecke,%20Nils&rft.date=2006-04-14&rft.volume=1111&rft.issue=2&rft.spage=206&rft.epage=213&rft.pages=206-213&rft.issn=0021-9673&rft_id=info:doi/10.1016/j.chroma.2005.05.100&rft_dat=%3Cproquest_cross%3E67813717%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67813717&rft_id=info:pmid/16569579&rft_els_id=S0021967305010733&rfr_iscdi=true |