Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal
The low luminal Ca 2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorptio...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-06, Vol.331 (4), p.1353-1357 |
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| creator | Yamauchi, Daisuke Raveendran, Nithya N. Pondugula, Satyanarayana R. Kampalli, Suresh B. Sanneman, Joel D. Harbidge, Donald G. Marcus, Daniel C. |
| description | The low luminal Ca
2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorption of Ca
2+ that is comprised of the epithelial Ca
2+ channels ECaC1 (CaT2, TRPV5) and ECaC2 (CaT1, TRPV6), calbindin (calbindin-D9k, calbindin-D28k), Na
+/Ca
2+ exchanger (NCX1, NCX2, and NCX3), and plasma membrane Ca
2+-ATPase (PMCA1, PMCA3, and PMCA4) by RT-PCR. Further, vitamin D receptor was also expressed in SCCD and it was found by quantitative RT-PCR that incubation for 24
h with 1,25-dihydroxyvitamin D
3 upregulated the expression of ECaC1, calbindin-D9k, and calbindin-D28k. These observations provide evidence for the first time of an ECaC-based Ca
2+ transport system in SCCD that could maintain the low Ca
2+ concentration in vestibular endolymph. |
| doi_str_mv | 10.1016/j.bbrc.2005.04.053 |
| format | Article |
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2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorption of Ca
2+ that is comprised of the epithelial Ca
2+ channels ECaC1 (CaT2, TRPV5) and ECaC2 (CaT1, TRPV6), calbindin (calbindin-D9k, calbindin-D28k), Na
+/Ca
2+ exchanger (NCX1, NCX2, and NCX3), and plasma membrane Ca
2+-ATPase (PMCA1, PMCA3, and PMCA4) by RT-PCR. Further, vitamin D receptor was also expressed in SCCD and it was found by quantitative RT-PCR that incubation for 24
h with 1,25-dihydroxyvitamin D
3 upregulated the expression of ECaC1, calbindin-D9k, and calbindin-D28k. These observations provide evidence for the first time of an ECaC-based Ca
2+ transport system in SCCD that could maintain the low Ca
2+ concentration in vestibular endolymph.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2005.04.053</identifier><identifier>PMID: 15883024</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>1,25-Dihydroxyvitamin D 3 ; Animals ; Base Sequence ; Calbindin ; Calcitriol - pharmacology ; Calcium Channels - genetics ; Calcium-Transporting ATPases - metabolism ; Cell Membrane - enzymology ; Cells, Cultured ; DNA Primers ; Epithelial calcium channel ; Na +/Ca 2+ exchanger ; Plasma membrane Ca 2+-ATPase ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Semicircular canal duct ; Semicircular Canals - metabolism ; Sodium-Calcium Exchanger - metabolism ; TRPV Cation Channels ; Up-Regulation - drug effects ; Vitamin D receptor</subject><ispartof>Biochemical and biophysical research communications, 2005-06, Vol.331 (4), p.1353-1357</ispartof><rights>2005 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-54a0aa1f982eecc7d5f96bcf94c2fe1ab7927e8537676cada9aef3ddcdd8ab5a3</citedby><cites>FETCH-LOGICAL-c385t-54a0aa1f982eecc7d5f96bcf94c2fe1ab7927e8537676cada9aef3ddcdd8ab5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X05008429$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15883024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamauchi, Daisuke</creatorcontrib><creatorcontrib>Raveendran, Nithya N.</creatorcontrib><creatorcontrib>Pondugula, Satyanarayana R.</creatorcontrib><creatorcontrib>Kampalli, Suresh B.</creatorcontrib><creatorcontrib>Sanneman, Joel D.</creatorcontrib><creatorcontrib>Harbidge, Donald G.</creatorcontrib><creatorcontrib>Marcus, Daniel C.</creatorcontrib><title>Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The low luminal Ca
2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorption of Ca
2+ that is comprised of the epithelial Ca
2+ channels ECaC1 (CaT2, TRPV5) and ECaC2 (CaT1, TRPV6), calbindin (calbindin-D9k, calbindin-D28k), Na
+/Ca
2+ exchanger (NCX1, NCX2, and NCX3), and plasma membrane Ca
2+-ATPase (PMCA1, PMCA3, and PMCA4) by RT-PCR. Further, vitamin D receptor was also expressed in SCCD and it was found by quantitative RT-PCR that incubation for 24
h with 1,25-dihydroxyvitamin D
3 upregulated the expression of ECaC1, calbindin-D9k, and calbindin-D28k. These observations provide evidence for the first time of an ECaC-based Ca
2+ transport system in SCCD that could maintain the low Ca
2+ concentration in vestibular endolymph.</description><subject>1,25-Dihydroxyvitamin D 3</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Calbindin</subject><subject>Calcitriol - pharmacology</subject><subject>Calcium Channels - genetics</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Cell Membrane - enzymology</subject><subject>Cells, Cultured</subject><subject>DNA Primers</subject><subject>Epithelial calcium channel</subject><subject>Na +/Ca 2+ exchanger</subject><subject>Plasma membrane Ca 2+-ATPase</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Semicircular canal duct</subject><subject>Semicircular Canals - metabolism</subject><subject>Sodium-Calcium Exchanger - metabolism</subject><subject>TRPV Cation Channels</subject><subject>Up-Regulation - drug effects</subject><subject>Vitamin D receptor</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAURoMoOj7-gAvpyl3rTZu0DbiR8QmiICruwm1yKxn6GJNW9N_bYQbc6epy4XxncRg75pBw4PnZIqkqb5IUQCYgEpDZFptxUBCnHMQ2mwFAHqeKv-2x_RAWAJyLXO2yPS7LMoNUzNjlqxuwdV10GY1LT-9jgwOFiL6mJwTXd1FfR1dznPOofXq4iCYyUOuM82ZCfWSww-aQ7dTYBDra3AP2cn31PL-N7x9v7uYX97HJSjnEUiAg8lqVKZExhZW1yitTK2HSmjhWhUoLKmVW5EVu0KJCqjNrjbUlVhKzA3a69i59_zFSGHTrgqGmwY76Mei8KFdh5L8gL0qRi0xNYLoGje9D8FTrpXct-m_NQa9ceqFXkfUqsgahp8jT6GRjH6uW7O9kU3UCztcATTE-HXkdjKPOkHWezKBt7_7y_wDYHY30</recordid><startdate>20050617</startdate><enddate>20050617</enddate><creator>Yamauchi, Daisuke</creator><creator>Raveendran, Nithya N.</creator><creator>Pondugula, Satyanarayana R.</creator><creator>Kampalli, Suresh B.</creator><creator>Sanneman, Joel D.</creator><creator>Harbidge, Donald G.</creator><creator>Marcus, Daniel C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20050617</creationdate><title>Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal</title><author>Yamauchi, Daisuke ; Raveendran, Nithya N. ; Pondugula, Satyanarayana R. ; Kampalli, Suresh B. ; Sanneman, Joel D. ; Harbidge, Donald G. ; Marcus, Daniel C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-54a0aa1f982eecc7d5f96bcf94c2fe1ab7927e8537676cada9aef3ddcdd8ab5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>1,25-Dihydroxyvitamin D 3</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Calbindin</topic><topic>Calcitriol - pharmacology</topic><topic>Calcium Channels - genetics</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Cell Membrane - enzymology</topic><topic>Cells, Cultured</topic><topic>DNA Primers</topic><topic>Epithelial calcium channel</topic><topic>Na +/Ca 2+ exchanger</topic><topic>Plasma membrane Ca 2+-ATPase</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Semicircular canal duct</topic><topic>Semicircular Canals - metabolism</topic><topic>Sodium-Calcium Exchanger - metabolism</topic><topic>TRPV Cation Channels</topic><topic>Up-Regulation - drug effects</topic><topic>Vitamin D receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamauchi, Daisuke</creatorcontrib><creatorcontrib>Raveendran, Nithya N.</creatorcontrib><creatorcontrib>Pondugula, Satyanarayana R.</creatorcontrib><creatorcontrib>Kampalli, Suresh B.</creatorcontrib><creatorcontrib>Sanneman, Joel D.</creatorcontrib><creatorcontrib>Harbidge, Donald G.</creatorcontrib><creatorcontrib>Marcus, Daniel C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamauchi, Daisuke</au><au>Raveendran, Nithya N.</au><au>Pondugula, Satyanarayana R.</au><au>Kampalli, Suresh B.</au><au>Sanneman, Joel D.</au><au>Harbidge, Donald G.</au><au>Marcus, Daniel C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-06-17</date><risdate>2005</risdate><volume>331</volume><issue>4</issue><spage>1353</spage><epage>1357</epage><pages>1353-1357</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The low luminal Ca
2+ concentration of mammalian endolymph in the vestibular labyrinth is required for normal balance. We found transcripts in primary cultures of semicircular canal duct (SCCD) epithelial cells from neonatal rats representing a complete transport system for transepithelial absorption of Ca
2+ that is comprised of the epithelial Ca
2+ channels ECaC1 (CaT2, TRPV5) and ECaC2 (CaT1, TRPV6), calbindin (calbindin-D9k, calbindin-D28k), Na
+/Ca
2+ exchanger (NCX1, NCX2, and NCX3), and plasma membrane Ca
2+-ATPase (PMCA1, PMCA3, and PMCA4) by RT-PCR. Further, vitamin D receptor was also expressed in SCCD and it was found by quantitative RT-PCR that incubation for 24
h with 1,25-dihydroxyvitamin D
3 upregulated the expression of ECaC1, calbindin-D9k, and calbindin-D28k. These observations provide evidence for the first time of an ECaC-based Ca
2+ transport system in SCCD that could maintain the low Ca
2+ concentration in vestibular endolymph.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15883024</pmid><doi>10.1016/j.bbrc.2005.04.053</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 2005-06, Vol.331 (4), p.1353-1357 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1,25-Dihydroxyvitamin D 3 Animals Base Sequence Calbindin Calcitriol - pharmacology Calcium Channels - genetics Calcium-Transporting ATPases - metabolism Cell Membrane - enzymology Cells, Cultured DNA Primers Epithelial calcium channel Na +/Ca 2+ exchanger Plasma membrane Ca 2+-ATPase Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Semicircular canal duct Semicircular Canals - metabolism Sodium-Calcium Exchanger - metabolism TRPV Cation Channels Up-Regulation - drug effects Vitamin D receptor |
| title | Vitamin D upregulates expression of ECaC1 mRNA in semicircular canal |
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