Functional Characterization of the Gene Encoding UDP-glucose: Tetrahydrobiopterin α-Glucosyltransferase in Synechococcus sp. PCC 7942
In this study, we attempted to characterize the Synechococcus sp. PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin α-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one...
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| Veröffentlicht in: | The journal of microbiology 2005-04, Vol.43 (2), p.191-195 |
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| creator | Cha, E.Y. (Inje University, Gimhae, Republic of Korea) Park, J.S. (Inje University, Gimhae, Republic of Korea) Jeon, S.R. (Inje University, Gimhae, Republic of Korea) Kong, J.S. (Inje University, Gimhae, Republic of Korea) Choi, Y.K. (Inje University, Gimhae, Republic of Korea) Ryu, J.Y. (Chungnam National University, Daejeon, Republic of Korea) Park, Y.I. (Chungnam National University, Daejeon, Republic of Korea) Park, Y.S. (Inje University, Gimhae, Republic of Korea), E-mail: mbyspark@inje.ac.kr |
| description | In this study, we attempted to characterize the Synechococcus sp. PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin α-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O₂ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells. |
| format | Article |
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PCC 7942</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Cha, E.Y. (Inje University, Gimhae, Republic of Korea) ; Park, J.S. (Inje University, Gimhae, Republic of Korea) ; Jeon, S.R. (Inje University, Gimhae, Republic of Korea) ; Kong, J.S. (Inje University, Gimhae, Republic of Korea) ; Choi, Y.K. (Inje University, Gimhae, Republic of Korea) ; Ryu, J.Y. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.I. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.S. (Inje University, Gimhae, Republic of Korea), E-mail: mbyspark@inje.ac.kr</creator><creatorcontrib>Cha, E.Y. (Inje University, Gimhae, Republic of Korea) ; Park, J.S. (Inje University, Gimhae, Republic of Korea) ; Jeon, S.R. (Inje University, Gimhae, Republic of Korea) ; Kong, J.S. (Inje University, Gimhae, Republic of Korea) ; Choi, Y.K. (Inje University, Gimhae, Republic of Korea) ; Ryu, J.Y. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.I. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.S. (Inje University, Gimhae, Republic of Korea), E-mail: mbyspark@inje.ac.kr</creatorcontrib><description>In this study, we attempted to characterize the Synechococcus sp. PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin α-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O₂ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells.</description><identifier>ISSN: 1225-8873</identifier><identifier>EISSN: 1976-3794</identifier><identifier>PMID: 15880096</identifier><language>eng</language><publisher>Korea (South)</publisher><subject>Bacterial Proteins - genetics ; CYANOBACTERIA ; FOTOSINTESIS ; Genes, Bacterial - physiology ; Glucosyltransferases - genetics ; photoprotection ; PHOTOSYNTHESE ; PHOTOSYNTHESIS ; phycocyanin rod linker ; Proteomics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Synechococcus - enzymology ; tetrahydrobiopterin-glucoside</subject><ispartof>The journal of microbiology, 2005-04, Vol.43 (2), p.191-195</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15880096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cha, E.Y. (Inje University, Gimhae, Republic of Korea)</creatorcontrib><creatorcontrib>Park, J.S. (Inje University, Gimhae, Republic of Korea)</creatorcontrib><creatorcontrib>Jeon, S.R. (Inje University, Gimhae, Republic of Korea)</creatorcontrib><creatorcontrib>Kong, J.S. (Inje University, Gimhae, Republic of Korea)</creatorcontrib><creatorcontrib>Choi, Y.K. (Inje University, Gimhae, Republic of Korea)</creatorcontrib><creatorcontrib>Ryu, J.Y. (Chungnam National University, Daejeon, Republic of Korea)</creatorcontrib><creatorcontrib>Park, Y.I. (Chungnam National University, Daejeon, Republic of Korea)</creatorcontrib><creatorcontrib>Park, Y.S. (Inje University, Gimhae, Republic of Korea), E-mail: mbyspark@inje.ac.kr</creatorcontrib><title>Functional Characterization of the Gene Encoding UDP-glucose: Tetrahydrobiopterin α-Glucosyltransferase in Synechococcus sp. PCC 7942</title><title>The journal of microbiology</title><addtitle>J Microbiol</addtitle><description>In this study, we attempted to characterize the Synechococcus sp. PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin α-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O₂ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells.</description><subject>Bacterial Proteins - genetics</subject><subject>CYANOBACTERIA</subject><subject>FOTOSINTESIS</subject><subject>Genes, Bacterial - physiology</subject><subject>Glucosyltransferases - genetics</subject><subject>photoprotection</subject><subject>PHOTOSYNTHESE</subject><subject>PHOTOSYNTHESIS</subject><subject>phycocyanin rod linker</subject><subject>Proteomics</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Synechococcus - enzymology</subject><subject>tetrahydrobiopterin-glucoside</subject><issn>1225-8873</issn><issn>1976-3794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kEFOwzAQRSMEoqVwBJBX7IJsJ7ETdii0BYFEBe06cuxxE5TaxU4W4QDch4twJlJaVjOa92Y0-kfBmGSchRHP4uOhpzQJ05RHo-DM-3eMGYliehqMSJKmGGdsHHzNOiPb2hrRoLwSTsgWXP0pdiNkNWorQHMwgKZGWlWbNVrdL8J100nr4RYtoXWi6pWzZW23u1WDfr7D-R_vmwEar8EJD2ggb70BWVlppew88tsbtMhzNPxKz4MTLRoPF4c6CVaz6TJ_CJ9f5o_53XOoaUTakCuhGOEpk4zGTMU6w5SUEgBImkgQVGUYyiTJQKcs0lzGieRJGRPFaclkFk2C6_3drbMfHfi22NReQtMIA7bzBeMp5ixig3h1ELtyA6rYunojXF_8JzcIl3tBC1uItat98fRKh4gxYThOol8AeXZn</recordid><startdate>200504</startdate><enddate>200504</enddate><creator>Cha, E.Y. 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(Inje University, Gimhae, Republic of Korea) ; Park, J.S. (Inje University, Gimhae, Republic of Korea) ; Jeon, S.R. (Inje University, Gimhae, Republic of Korea) ; Kong, J.S. (Inje University, Gimhae, Republic of Korea) ; Choi, Y.K. (Inje University, Gimhae, Republic of Korea) ; Ryu, J.Y. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.I. (Chungnam National University, Daejeon, Republic of Korea) ; Park, Y.S. 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PCC 7942 mutant resultant from a disruption in the gene encoding UDP-glucose: tetrahydrobiopterin α-glucosyltransferase (BGluT). 2D-PAGE followed by MALDI-TOF mass spectrometry revealed that phycocyanin rod linker protein 33K was one of the proteins expressed at lower level in the BGluT mutant. BGluT mutant cells were also determined to be more sensitive to high light stress. This is because photosynthetic O₂ exchange rates were significantly decreased, due to the reduced number of functional PSIs relative to the wild type cells.</abstract><cop>Korea (South)</cop><pmid>15880096</pmid><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 1225-8873 |
| ispartof | The journal of microbiology, 2005-04, Vol.43 (2), p.191-195 |
| issn | 1225-8873 1976-3794 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Bacterial Proteins - genetics CYANOBACTERIA FOTOSINTESIS Genes, Bacterial - physiology Glucosyltransferases - genetics photoprotection PHOTOSYNTHESE PHOTOSYNTHESIS phycocyanin rod linker Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Synechococcus - enzymology tetrahydrobiopterin-glucoside |
| title | Functional Characterization of the Gene Encoding UDP-glucose: Tetrahydrobiopterin α-Glucosyltransferase in Synechococcus sp. PCC 7942 |
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