Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, People's Republic of China Correspondence Zongze Shao shaozz{at}163.com Two bacterial strains, B-5 T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sedimen...

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Veröffentlicht in:International journal of systematic and evolutionary microbiology 2005-05, Vol.55 (3), p.1181-1186
Hauptverfasser: Liu, Chenli, Shao, Zongze
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description Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, People's Republic of China Correspondence Zongze Shao shaozz{at}163.com Two bacterial strains, B-5 T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5 T and NO1A were shown to belong to the - Proteobacteria . Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C 16 : 0 , C 16 : 1 7 c and C 18 : 1 7 c . The chemotaxonomically characteristic fatty acid C 19 : 0 cyclo 8 c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5 T and NO1A is proposed, with the type strain B-5 T (=DSM 16502 T =CGMCC 1.3690 T ). Abbreviations: AlkB ( alkB ), alkane hydroxylase; ITS, internally transcribed spacer The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are AY683537 ( A. dieselolei B-5 T , 16S rRNA gene), AY683538 (B-5 T , large ITS), AY683539 (B-5 T , small ITS), AY683540 (B-5 T , partial alkB gene), AY683531 ( A. dieselolei NO1A, 16S rRNA gene), AY683532 (NO1A, large ITS), AY683533 (NO1A, small ITS), AY683534 (NO1A, partial alkB gene), AY683536 ( A. jadensis T9 T , partial alkB gene) and AY683535 ( A. venustensis ISO4 T , partial alkB gene). Transmission electron micrographs of cells of strains B-5 T and NO1A and dendrograms showing the phylogenetic positions of the two strains plus recognized members of the genus Alcanivorax based on 16S rRNA, ITS and alkB gene sequences are available as supplementary figures in IJSEM Online, together with a table giving DNA–DNA relatedness values.
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Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5 T and NO1A were shown to belong to the - Proteobacteria . Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C 16 : 0 , C 16 : 1 7 c and C 18 : 1 7 c . The chemotaxonomically characteristic fatty acid C 19 : 0 cyclo 8 c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5 T and NO1A is proposed, with the type strain B-5 T (=DSM 16502 T =CGMCC 1.3690 T ). Abbreviations: AlkB ( alkB ), alkane hydroxylase; ITS, internally transcribed spacer The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are AY683537 ( A. dieselolei B-5 T , 16S rRNA gene), AY683538 (B-5 T , large ITS), AY683539 (B-5 T , small ITS), AY683540 (B-5 T , partial alkB gene), AY683531 ( A. dieselolei NO1A, 16S rRNA gene), AY683532 (NO1A, large ITS), AY683533 (NO1A, small ITS), AY683534 (NO1A, partial alkB gene), AY683536 ( A. jadensis T9 T , partial alkB gene) and AY683535 ( A. venustensis ISO4 T , partial alkB gene). Transmission electron micrographs of cells of strains B-5 T and NO1A and dendrograms showing the phylogenetic positions of the two strains plus recognized members of the genus Alcanivorax based on 16S rRNA, ITS and alkB gene sequences are available as supplementary figures in IJSEM Online, together with a table giving DNA–DNA relatedness values.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.63443-0</identifier><identifier>PMID: 15879252</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Aerobiosis ; Alkanes - metabolism ; Animal, plant and microbial ecology ; Bacteriology ; Base Composition ; Biological and medical sciences ; Carboxylic Acids - metabolism ; Catalase - analysis ; DNA, Bacterial - chemistry ; DNA, Bacterial - isolation &amp; purification ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - isolation &amp; purification ; DNA, Ribosomal Spacer - genetics ; Fatty Acids - analysis ; Fatty Acids - isolation &amp; purification ; Fundamental and applied biological sciences. Psychology ; Genes, rRNA ; Gentian Violet ; Geologic Sediments - microbiology ; Growth Inhibitors - pharmacology ; Halomonadaceae - classification ; Halomonadaceae - cytology ; Halomonadaceae - isolation &amp; purification ; Halomonadaceae - metabolism ; Hydrolases - genetics ; Marine ; Microbial ecology ; Microbiology ; Molecular Sequence Data ; Movement ; Nucleic Acid Hybridization ; Oxidoreductases - analysis ; Pacific Ocean ; Phenazines ; Phylogeny ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Seawater - microbiology ; Sequence Analysis, DNA ; Sodium Chloride - pharmacology ; Systematics ; Various environments (extraatmospheric space, air, water) ; Water Microbiology</subject><ispartof>International journal of systematic and evolutionary microbiology, 2005-05, Vol.55 (3), p.1181-1186</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-a9cf0da29522125995e579f7de398deecaf218d0da9d8c0bae9f8d53d74e10a83</citedby><cites>FETCH-LOGICAL-c490t-a9cf0da29522125995e579f7de398deecaf218d0da9d8c0bae9f8d53d74e10a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3746,3747,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=16847420$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15879252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Chenli</creatorcontrib><creatorcontrib>Shao, Zongze</creatorcontrib><title>Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, People's Republic of China Correspondence Zongze Shao shaozz{at}163.com Two bacterial strains, B-5 T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5 T and NO1A were shown to belong to the - Proteobacteria . Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C 16 : 0 , C 16 : 1 7 c and C 18 : 1 7 c . The chemotaxonomically characteristic fatty acid C 19 : 0 cyclo 8 c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5 T and NO1A is proposed, with the type strain B-5 T (=DSM 16502 T =CGMCC 1.3690 T ). Abbreviations: AlkB ( alkB ), alkane hydroxylase; ITS, internally transcribed spacer The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are AY683537 ( A. dieselolei B-5 T , 16S rRNA gene), AY683538 (B-5 T , large ITS), AY683539 (B-5 T , small ITS), AY683540 (B-5 T , partial alkB gene), AY683531 ( A. dieselolei NO1A, 16S rRNA gene), AY683532 (NO1A, large ITS), AY683533 (NO1A, small ITS), AY683534 (NO1A, partial alkB gene), AY683536 ( A. jadensis T9 T , partial alkB gene) and AY683535 ( A. venustensis ISO4 T , partial alkB gene). Transmission electron micrographs of cells of strains B-5 T and NO1A and dendrograms showing the phylogenetic positions of the two strains plus recognized members of the genus Alcanivorax based on 16S rRNA, ITS and alkB gene sequences are available as supplementary figures in IJSEM Online, together with a table giving DNA–DNA relatedness values.</description><subject>Aerobiosis</subject><subject>Alkanes - metabolism</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteriology</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>Carboxylic Acids - metabolism</subject><subject>Catalase - analysis</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - isolation &amp; purification</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - isolation &amp; purification</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - isolation &amp; purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, rRNA</subject><subject>Gentian Violet</subject><subject>Geologic Sediments - microbiology</subject><subject>Growth Inhibitors - pharmacology</subject><subject>Halomonadaceae - classification</subject><subject>Halomonadaceae - cytology</subject><subject>Halomonadaceae - isolation &amp; purification</subject><subject>Halomonadaceae - metabolism</subject><subject>Hydrolases - genetics</subject><subject>Marine</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Movement</subject><subject>Nucleic Acid Hybridization</subject><subject>Oxidoreductases - analysis</subject><subject>Pacific Ocean</subject><subject>Phenazines</subject><subject>Phylogeny</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Seawater - microbiology</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium Chloride - pharmacology</subject><subject>Systematics</subject><subject>Various environments (extraatmospheric space, air, water)</subject><subject>Water Microbiology</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EomVgyRZ5A2JBgh9xYi-ripdUiQ2srTv2zdTFiad2pi3_HocZ0SWr-9Cncx-HkNectZwZ8zHclJa1vew62bAn5Jx3fd8oJrun_3LRn5EXpdwwVhuMPSdnXOnBCCXOye1FdDCHu5ThgfqABWOKGGjZt3ROd-0HCmvESCH-ghkbj7sMPsw7ugW3YA6HiYaSIizo6ZjTRAsCva9lpjB76hH3zdoq6MOE8_KSPBshFnx1ihvy8_OnH5dfm6vvX75dXlw1rjNsacC4kXkQRgnBhTJGoRrMOHiURldRB6Pg2lfEeO3YFtCM2ivphw45Ay035N1Rd5_T7QHLYqdQHMZYr0iHYvtBs0FI8V-QD72Qff3vhjRH0OVUSsbR7nOYIP-2nNnVDFvNsMz-NcOyyr85CR-2E_pH-vT9Crw9AVAcxDHD7EJ55HrdDZ1Yhd4fueuwu74PGe0O5ynUNbYhrUOVstJyrrn8AzHDoag</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Liu, Chenli</creator><creator>Shao, Zongze</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment</title><author>Liu, Chenli ; Shao, Zongze</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-a9cf0da29522125995e579f7de398deecaf218d0da9d8c0bae9f8d53d74e10a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aerobiosis</topic><topic>Alkanes - metabolism</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteriology</topic><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>Carboxylic Acids - metabolism</topic><topic>Catalase - analysis</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - isolation &amp; purification</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - isolation &amp; purification</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - isolation &amp; purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, rRNA</topic><topic>Gentian Violet</topic><topic>Geologic Sediments - microbiology</topic><topic>Growth Inhibitors - pharmacology</topic><topic>Halomonadaceae - classification</topic><topic>Halomonadaceae - cytology</topic><topic>Halomonadaceae - isolation &amp; purification</topic><topic>Halomonadaceae - metabolism</topic><topic>Hydrolases - genetics</topic><topic>Marine</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Movement</topic><topic>Nucleic Acid Hybridization</topic><topic>Oxidoreductases - analysis</topic><topic>Pacific Ocean</topic><topic>Phenazines</topic><topic>Phylogeny</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Seawater - microbiology</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium Chloride - pharmacology</topic><topic>Systematics</topic><topic>Various environments (extraatmospheric space, air, water)</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Chenli</creatorcontrib><creatorcontrib>Shao, Zongze</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) 1: Biological Sciences &amp; Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science &amp; Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Chenli</au><au>Shao, Zongze</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>55</volume><issue>3</issue><spage>1181</spage><epage>1186</epage><pages>1181-1186</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, People's Republic of China Correspondence Zongze Shao shaozz{at}163.com Two bacterial strains, B-5 T and NO1A, were isolated from the surface water of the Bohai Sea and deep-sea sediment of the east Pacific Ocean, respectively. Both strains were halophilic, aerobic, Gram-negative, non-spore-forming, catalase- and oxidase-positive motile rods. They grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. On the basis of 16S rRNA gene sequence similarity, strains B-5 T and NO1A were shown to belong to the - Proteobacteria . Highest similarity values were found with Alcanivorax venustensis (95·2 %), Alcanivorax jadensis (94·6 %) and Alcanivorax borkumensis (94·1 %). Principal fatty acids of both strains were C 16 : 0 , C 16 : 1 7 c and C 18 : 1 7 c . The chemotaxonomically characteristic fatty acid C 19 : 0 cyclo 8 c was also detected. On the basis of the above, together with results of physiological and biochemical tests, DNA–DNA hybridization, comparisons of 16S–23S internal transcribed spacer sequences and comparisons of the partial deduced amino acid sequence of alkane hydroxylase, both strains were affiliated to the genus Alcanivorax but were differentiated from recognized Alcanivorax species. Therefore, a novel species, Alcanivorax dieselolei sp. nov., represented by strains B-5 T and NO1A is proposed, with the type strain B-5 T (=DSM 16502 T =CGMCC 1.3690 T ). Abbreviations: AlkB ( alkB ), alkane hydroxylase; ITS, internally transcribed spacer The GenBank/EMBL/DDBJ accession numbers for the nucleotide sequences reported in this study are AY683537 ( A. dieselolei B-5 T , 16S rRNA gene), AY683538 (B-5 T , large ITS), AY683539 (B-5 T , small ITS), AY683540 (B-5 T , partial alkB gene), AY683531 ( A. dieselolei NO1A, 16S rRNA gene), AY683532 (NO1A, large ITS), AY683533 (NO1A, small ITS), AY683534 (NO1A, partial alkB gene), AY683536 ( A. jadensis T9 T , partial alkB gene) and AY683535 ( A. venustensis ISO4 T , partial alkB gene). Transmission electron micrographs of cells of strains B-5 T and NO1A and dendrograms showing the phylogenetic positions of the two strains plus recognized members of the genus Alcanivorax based on 16S rRNA, ITS and alkB gene sequences are available as supplementary figures in IJSEM Online, together with a table giving DNA–DNA relatedness values.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>15879252</pmid><doi>10.1099/ijs.0.63443-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Aerobiosis
Alkanes - metabolism
Animal, plant and microbial ecology
Bacteriology
Base Composition
Biological and medical sciences
Carboxylic Acids - metabolism
Catalase - analysis
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - isolation & purification
DNA, Ribosomal Spacer - genetics
Fatty Acids - analysis
Fatty Acids - isolation & purification
Fundamental and applied biological sciences. Psychology
Genes, rRNA
Gentian Violet
Geologic Sediments - microbiology
Growth Inhibitors - pharmacology
Halomonadaceae - classification
Halomonadaceae - cytology
Halomonadaceae - isolation & purification
Halomonadaceae - metabolism
Hydrolases - genetics
Marine
Microbial ecology
Microbiology
Molecular Sequence Data
Movement
Nucleic Acid Hybridization
Oxidoreductases - analysis
Pacific Ocean
Phenazines
Phylogeny
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Seawater - microbiology
Sequence Analysis, DNA
Sodium Chloride - pharmacology
Systematics
Various environments (extraatmospheric space, air, water)
Water Microbiology
title Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment
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