Presentation of the HCV-derived lipopeptide LP20–44 by dendritic cells enhances function of in vitro-generated CD4 + T cells via up-regulation of TLR2
In vitro immunization with HCV lipopeptide aa 20–44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-γ secretion upon H...
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| Veröffentlicht in: | Vaccine 2006-04, Vol.24 (16), p.3066-3075 |
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| creator | Langhans, Bettina Braunschweiger, Ingrid Nischalke, Hans-Dieter Nattermann, Jacob Sauerbruch, Tilman Spengler, Ulrich |
| description | In vitro immunization with HCV lipopeptide aa 20–44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-γ secretion upon HCV-specific re-stimulation. This effect was related to significantly increased expression of TLR2 on DC-immunized CD4
+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8
+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4
+ T cells but not CD8
+ T cells. |
| doi_str_mv | 10.1016/j.vaccine.2006.01.057 |
| format | Article |
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+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8
+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4
+ T cells but not CD8
+ T cells.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2006.01.057</identifier><identifier>PMID: 16504351</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic ; Adult ; Amino acids ; Antigen Presentation ; Applied microbiology ; Biological and medical sciences ; CD4 + T cells ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; Cell Proliferation ; Cysteine - analogs & derivatives ; Dendritic Cells - immunology ; Experiments ; Female ; Fundamental and applied biological sciences. Psychology ; Granzymes ; Haplotypes ; Hepacivirus - immunology ; Hepatitis ; Hepatitis C virus (HCV) ; Humans ; Immunization ; Interferon-gamma - biosynthesis ; Lipidated peptide ; Lipoproteins - immunology ; Lymphocyte Subsets - immunology ; Lymphocytes ; Male ; Microbiology ; Middle Aged ; Miscellaneous ; Peptides ; Serine Endopeptidases - analysis ; Toll-Like Receptor 2 - biosynthesis ; Up-Regulation ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Viral Proteins - chemistry ; Viral Proteins - immunology ; Virology</subject><ispartof>Vaccine, 2006-04, Vol.24 (16), p.3066-3075</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><rights>Copyright Elsevier Limited Apr 12, 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-782f5009beb5bc7e3c6cf82cc1bfb9141d0a29d6314b96550d248196a6cf338a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1547143671?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17666010$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16504351$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Langhans, Bettina</creatorcontrib><creatorcontrib>Braunschweiger, Ingrid</creatorcontrib><creatorcontrib>Nischalke, Hans-Dieter</creatorcontrib><creatorcontrib>Nattermann, Jacob</creatorcontrib><creatorcontrib>Sauerbruch, Tilman</creatorcontrib><creatorcontrib>Spengler, Ulrich</creatorcontrib><title>Presentation of the HCV-derived lipopeptide LP20–44 by dendritic cells enhances function of in vitro-generated CD4 + T cells via up-regulation of TLR2</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>In vitro immunization with HCV lipopeptide aa 20–44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-γ secretion upon HCV-specific re-stimulation. This effect was related to significantly increased expression of TLR2 on DC-immunized CD4
+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8
+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4
+ T cells but not CD8
+ T cells.</description><subject>Adjuvants, Immunologic</subject><subject>Adult</subject><subject>Amino acids</subject><subject>Antigen Presentation</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>CD4 + T cells</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cell Proliferation</subject><subject>Cysteine - analogs & derivatives</subject><subject>Dendritic Cells - immunology</subject><subject>Experiments</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Langhans, Bettina</au><au>Braunschweiger, Ingrid</au><au>Nischalke, Hans-Dieter</au><au>Nattermann, Jacob</au><au>Sauerbruch, Tilman</au><au>Spengler, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presentation of the HCV-derived lipopeptide LP20–44 by dendritic cells enhances function of in vitro-generated CD4 + T cells via up-regulation of TLR2</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>2006-04-12</date><risdate>2006</risdate><volume>24</volume><issue>16</issue><spage>3066</spage><epage>3075</epage><pages>3066-3075</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>In vitro immunization with HCV lipopeptide aa 20–44 results in antigen-specific T cells. Here, we studied whether presentation of the lipopeptide by dendritic cells (DC) results in enhanced T cell functions. DC-immunized T cells showed significantly augmented proliferation and IFN-γ secretion upon HCV-specific re-stimulation. This effect was related to significantly increased expression of TLR2 on DC-immunized CD4
+ T cells and required TLR2 triggering during re-stimulation. In contrast, numbers of HLA-A2-pentamer-positive CD8
+ T cells and granzyme B-secreting T cells remained unchanged. Thus, up-regulation of TLR2 during immunization with DC enhances functions of CD4
+ T cells but not CD8
+ T cells.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16504351</pmid><doi>10.1016/j.vaccine.2006.01.057</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Vaccine, 2006-04, Vol.24 (16), p.3066-3075 |
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| subjects | Adjuvants, Immunologic Adult Amino acids Antigen Presentation Applied microbiology Biological and medical sciences CD4 + T cells CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology Cell Proliferation Cysteine - analogs & derivatives Dendritic Cells - immunology Experiments Female Fundamental and applied biological sciences. Psychology Granzymes Haplotypes Hepacivirus - immunology Hepatitis Hepatitis C virus (HCV) Humans Immunization Interferon-gamma - biosynthesis Lipidated peptide Lipoproteins - immunology Lymphocyte Subsets - immunology Lymphocytes Male Microbiology Middle Aged Miscellaneous Peptides Serine Endopeptidases - analysis Toll-Like Receptor 2 - biosynthesis Up-Regulation Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral Proteins - chemistry Viral Proteins - immunology Virology |
| title | Presentation of the HCV-derived lipopeptide LP20–44 by dendritic cells enhances function of in vitro-generated CD4 + T cells via up-regulation of TLR2 |
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