Delivery of TEM β-lactamase by gene-transformed Lactococcus lactis subsp. lactis through cervical cell monolayer

Lactococcus lactis subsp. lactis transformed with Plasmid ss80 (encoding the production and secretion of TEM β-lactamase) was used for the delivery of β-lactamase through the C-33A (cervix cell) monolayer. The viability of the cell monolayers co-cultured with L. lactis was examined by the trypan blu...

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Veröffentlicht in:	International journal of pharmaceutics 2006-04, Vol.313 (1), p.29-35
Hauptverfasser:	Kaushal, Gagan, Trombetta, Louis, Ochs, Raymond S., Shao, Jun
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Adhesion beta-Lactamases - genetics beta-Lactamases - metabolism Bioavailability Biological and medical sciences C-33A Cell Line, Tumor Cervix Uteri - cytology Cervix Uteri - metabolism Epithelial Cells - metabolism Female General pharmacology Humans Lactococcus lactis Lactococcus lactis - genetics Lactococcus lactis - metabolism Medical sciences Normal flora Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Protein delivery TEM β-lactamase Time Factors Transformation, Bacterial Vaginal delivery
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