Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte antigen-C-reactive antibodies in kidney transplant recipients
Human leukocyte antigen (HLA)-C is expressed on nucleated cells and platelets in lower levels than HLA-A,B, and its antigens are in linkage disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is difficult. The authors questioned whether HLA-C could serve as a target in clinical k...
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| Veröffentlicht in: | Transplantation 2005-05, Vol.79 (9), p.1268-1272 |
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| creator | ZOET, Yvonne M EIJSINK, Chantal BBHTNOVD, Radka WITVLIET, Marian D KARDOL, Marrie J FRANKE, Marry E. I CLAAS, Frans H. J MULDER, Arend DOXIADIS, Ilias I. N |
| description | Human leukocyte antigen (HLA)-C is expressed on nucleated cells and platelets in lower levels than HLA-A,B, and its antigens are in linkage disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is difficult. The authors questioned whether HLA-C could serve as a target in clinical kidney transplantation using a newly developed assay.
Flow cytometry was performed with sera from patients (n=34) awaiting a kidney retransplant using nine cell lines expressing a single HLA-C antigen (single-antigen lines [SAL]).
The SAL were validated with HLA-C-specific alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The results were in agreement with the specificities previously reported. Exceptions, because of new HLA-C specificities used here, could be explained by epitope sharing between the antigens. With respect to patient sera, 15 of the 34 patients tested (44%) showed serum reactivity toward one or more HLA-C SAL.
In contrast to peripheral blood lymphocytes, SAL are excellent targets for detecting HLA-C-reactive alloantibodies by flow cytometry. This preliminary analysis revealed that HLA-C-reactive antibodies are frequently present in sera of retransplant patients, serving as possible targets in clinical transplantation. |
| doi_str_mv | 10.1097/01.TP.0000161246.33019.B6 |
| format | Article |
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Flow cytometry was performed with sera from patients (n=34) awaiting a kidney retransplant using nine cell lines expressing a single HLA-C antigen (single-antigen lines [SAL]).
The SAL were validated with HLA-C-specific alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The results were in agreement with the specificities previously reported. Exceptions, because of new HLA-C specificities used here, could be explained by epitope sharing between the antigens. With respect to patient sera, 15 of the 34 patients tested (44%) showed serum reactivity toward one or more HLA-C SAL.
In contrast to peripheral blood lymphocytes, SAL are excellent targets for detecting HLA-C-reactive alloantibodies by flow cytometry. This preliminary analysis revealed that HLA-C-reactive antibodies are frequently present in sera of retransplant patients, serving as possible targets in clinical transplantation.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/01.TP.0000161246.33019.B6</identifier><identifier>PMID: 15880083</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott</publisher><subject>Antibodies - blood ; Antigens - immunology ; Biological and medical sciences ; Biomarkers - analysis ; Cell Line ; Epitopes - analysis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; HLA-C Antigens - immunology ; Humans ; Kidney Transplantation - immunology ; Leukocytes - immunology ; Medical sciences ; Patient Selection ; Reproducibility of Results ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tissue, organ and graft immunology</subject><ispartof>Transplantation, 2005-05, Vol.79 (9), p.1268-1272</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-acc86d78d320f7c01b6b99fa84bd5cc9aa2d089486116874bd5aee6ba18adb283</citedby><cites>FETCH-LOGICAL-c427t-acc86d78d320f7c01b6b99fa84bd5cc9aa2d089486116874bd5aee6ba18adb283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16808763$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15880083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ZOET, Yvonne M</creatorcontrib><creatorcontrib>EIJSINK, Chantal</creatorcontrib><creatorcontrib>BBHTNOVD, Radka</creatorcontrib><creatorcontrib>WITVLIET, Marian D</creatorcontrib><creatorcontrib>KARDOL, Marrie J</creatorcontrib><creatorcontrib>FRANKE, Marry E. I</creatorcontrib><creatorcontrib>CLAAS, Frans H. J</creatorcontrib><creatorcontrib>MULDER, Arend</creatorcontrib><creatorcontrib>DOXIADIS, Ilias I. N</creatorcontrib><title>Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte antigen-C-reactive antibodies in kidney transplant recipients</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Human leukocyte antigen (HLA)-C is expressed on nucleated cells and platelets in lower levels than HLA-A,B, and its antigens are in linkage disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is difficult. The authors questioned whether HLA-C could serve as a target in clinical kidney transplantation using a newly developed assay.
Flow cytometry was performed with sera from patients (n=34) awaiting a kidney retransplant using nine cell lines expressing a single HLA-C antigen (single-antigen lines [SAL]).
The SAL were validated with HLA-C-specific alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The results were in agreement with the specificities previously reported. Exceptions, because of new HLA-C specificities used here, could be explained by epitope sharing between the antigens. With respect to patient sera, 15 of the 34 patients tested (44%) showed serum reactivity toward one or more HLA-C SAL.
In contrast to peripheral blood lymphocytes, SAL are excellent targets for detecting HLA-C-reactive alloantibodies by flow cytometry. This preliminary analysis revealed that HLA-C-reactive antibodies are frequently present in sera of retransplant patients, serving as possible targets in clinical transplantation.</description><subject>Antibodies - blood</subject><subject>Antigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Cell Line</subject><subject>Epitopes - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>HLA-C Antigens - immunology</subject><subject>Humans</subject><subject>Kidney Transplantation - immunology</subject><subject>Leukocytes - immunology</subject><subject>Medical sciences</subject><subject>Patient Selection</subject><subject>Reproducibility of Results</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. 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N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte antigen-C-reactive antibodies in kidney transplant recipients</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2005-05-15</date><risdate>2005</risdate><volume>79</volume><issue>9</issue><spage>1268</spage><epage>1272</epage><pages>1268-1272</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Human leukocyte antigen (HLA)-C is expressed on nucleated cells and platelets in lower levels than HLA-A,B, and its antigens are in linkage disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is difficult. The authors questioned whether HLA-C could serve as a target in clinical kidney transplantation using a newly developed assay.
Flow cytometry was performed with sera from patients (n=34) awaiting a kidney retransplant using nine cell lines expressing a single HLA-C antigen (single-antigen lines [SAL]).
The SAL were validated with HLA-C-specific alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The results were in agreement with the specificities previously reported. Exceptions, because of new HLA-C specificities used here, could be explained by epitope sharing between the antigens. With respect to patient sera, 15 of the 34 patients tested (44%) showed serum reactivity toward one or more HLA-C SAL.
In contrast to peripheral blood lymphocytes, SAL are excellent targets for detecting HLA-C-reactive alloantibodies by flow cytometry. This preliminary analysis revealed that HLA-C-reactive antibodies are frequently present in sera of retransplant patients, serving as possible targets in clinical transplantation.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>15880083</pmid><doi>10.1097/01.TP.0000161246.33019.B6</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Transplantation, 2005-05, Vol.79 (9), p.1268-1272 |
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| language | eng |
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| subjects | Antibodies - blood Antigens - immunology Biological and medical sciences Biomarkers - analysis Cell Line Epitopes - analysis Fundamental and applied biological sciences. Psychology Fundamental immunology HLA-C Antigens - immunology Humans Kidney Transplantation - immunology Leukocytes - immunology Medical sciences Patient Selection Reproducibility of Results Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue, organ and graft immunology |
| title | Single-antigen-expressing cell lines are excellent tools for detecting human leukocyte antigen-C-reactive antibodies in kidney transplant recipients |
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