Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK
Leukocyte preparations from children with documented evidence of MMR vaccination and confirmed diagnosis of autism were examined by several assays designed to target multiple regions of the measles virus genome sequence. No sample was found positive by any method. The assays applied were highly sens...
Gespeichert in:
| Veröffentlicht in: | Journal of medical virology 2006-05, Vol.78 (5), p.623-630 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 630 |
|---|---|
| container_issue | 5 |
| container_start_page | 623 |
| container_title | Journal of medical virology |
| container_volume | 78 |
| creator | Afzal, M.A. Ozoemena, L.C. O'Hare, A. Kidger, K.A. Bentley, M.L. Minor, P.D. |
| description | Leukocyte preparations from children with documented evidence of MMR vaccination and confirmed diagnosis of autism were examined by several assays designed to target multiple regions of the measles virus genome sequence. No sample was found positive by any method. The assays applied were highly sensitive, specific and robust in nature, and were based on the amplification of measles virus RNA transcripts by real‐time quantitative RT‐PCR (QRT‐PCR) as well as by conventional RT‐PCR‐nested PCR. The assays applied were potentially able to detect measles virus RNA down to single figure copy numbers per reaction. The amount of total nucleic acid extract of leukocytes subjected to various measles virus‐specific investigations was several fold higher than minimally required of a sample where measles virus persistence is well documented. This study failed to substantiate reports of the persistence of measles virus in autistic children with development regression. J. Med. Virol. 78:623–630, 2006. © 2006 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jmv.20585 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67800160</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20252088</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4225-1a1ed609e1ab1428836b520ab4e41e34696fbce6d31cb7d44c7d7faafacfca153</originalsourceid><addsrcrecordid>eNqFkcFu1DAURS0EotOBBT-AvAGJRVrbie3Msq1oS-mARCmV2FiO_dK4JE6xkynln_hHPJOBrhAbv4XPvfc9XYReULJHCWH7N91qjxFe8kdoRslCZAsi6WM0I7QQmRCU76DdGG8IIeWCsadohwrOOZN0hn4dVBG8AdzX2MIAZtBVC7gDHVuIeOXCGPE1-L4DHOH7uGGdx1Xb93Yt0uPg4uAMNo1rbQCP75oeN3oF6bF4aMAFvFx-wittjPN6cL3HdgzOX68_ceiTgYdJ3qxNXdeN3v2cyGgasGO72e_y_TP0pNZthOfbOUeXx28_H51m5x9P3h0dnGemYIxnVFOwgiyA6ooWrCxzUXFGdFVAQSEvxELUlQFhc2oqaYvCSCtrrWttaqMpz-fo9eR7G_p0cxxU56KBttUe-jEqIUtCqCD_BRlhKTgtMEdvJtCEPsYAtboNrtPhXlGi1iWqVKLalJjYl1vTserAPpDb1hLwagvoaHRbB-2Niw-cFJILLhO3P3F3roX7fyeqs-WXP9HZpEidwo-_Ch2-pZtzydXVhxN1dXhxfHb4VaqL_DdEesaM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20252088</pqid></control><display><type>article</type><title>Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Afzal, M.A. ; Ozoemena, L.C. ; O'Hare, A. ; Kidger, K.A. ; Bentley, M.L. ; Minor, P.D.</creator><creatorcontrib>Afzal, M.A. ; Ozoemena, L.C. ; O'Hare, A. ; Kidger, K.A. ; Bentley, M.L. ; Minor, P.D.</creatorcontrib><description>Leukocyte preparations from children with documented evidence of MMR vaccination and confirmed diagnosis of autism were examined by several assays designed to target multiple regions of the measles virus genome sequence. No sample was found positive by any method. The assays applied were highly sensitive, specific and robust in nature, and were based on the amplification of measles virus RNA transcripts by real‐time quantitative RT‐PCR (QRT‐PCR) as well as by conventional RT‐PCR‐nested PCR. The assays applied were potentially able to detect measles virus RNA down to single figure copy numbers per reaction. The amount of total nucleic acid extract of leukocytes subjected to various measles virus‐specific investigations was several fold higher than minimally required of a sample where measles virus persistence is well documented. This study failed to substantiate reports of the persistence of measles virus in autistic children with development regression. J. Med. Virol. 78:623–630, 2006. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.20585</identifier><identifier>PMID: 16555271</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; autism ; Autistic Disorder - etiology ; Biological and medical sciences ; Child ; Child, Preschool ; Female ; Fundamental and applied biological sciences. Psychology ; Genome, Viral ; Human viral diseases ; Humans ; Infectious diseases ; Leukocytes - virology ; Male ; Measles - blood ; Measles - complications ; Measles - prevention & control ; Measles virus ; Measles virus - genetics ; Measles virus - isolation & purification ; Measles-Mumps-Rubella Vaccine - adverse effects ; Medical sciences ; Microbiology ; Miscellaneous ; Polymerase Chain Reaction ; real-time quantitative RT-PCR ; RNA, Viral - blood ; RNA, Viral - genetics ; United Kingdom ; Vaccination - adverse effects ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Viral diseases ; Virology</subject><ispartof>Journal of medical virology, 2006-05, Vol.78 (5), p.623-630</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><rights>2006 INIST-CNRS</rights><rights>Copyright 2006 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4225-1a1ed609e1ab1428836b520ab4e41e34696fbce6d31cb7d44c7d7faafacfca153</citedby><cites>FETCH-LOGICAL-c4225-1a1ed609e1ab1428836b520ab4e41e34696fbce6d31cb7d44c7d7faafacfca153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.20585$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.20585$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17675657$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16555271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Afzal, M.A.</creatorcontrib><creatorcontrib>Ozoemena, L.C.</creatorcontrib><creatorcontrib>O'Hare, A.</creatorcontrib><creatorcontrib>Kidger, K.A.</creatorcontrib><creatorcontrib>Bentley, M.L.</creatorcontrib><creatorcontrib>Minor, P.D.</creatorcontrib><title>Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>Leukocyte preparations from children with documented evidence of MMR vaccination and confirmed diagnosis of autism were examined by several assays designed to target multiple regions of the measles virus genome sequence. No sample was found positive by any method. The assays applied were highly sensitive, specific and robust in nature, and were based on the amplification of measles virus RNA transcripts by real‐time quantitative RT‐PCR (QRT‐PCR) as well as by conventional RT‐PCR‐nested PCR. The assays applied were potentially able to detect measles virus RNA down to single figure copy numbers per reaction. The amount of total nucleic acid extract of leukocytes subjected to various measles virus‐specific investigations was several fold higher than minimally required of a sample where measles virus persistence is well documented. This study failed to substantiate reports of the persistence of measles virus in autistic children with development regression. J. Med. Virol. 78:623–630, 2006. © 2006 Wiley‐Liss, Inc.</description><subject>Adolescent</subject><subject>autism</subject><subject>Autistic Disorder - etiology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genome, Viral</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Leukocytes - virology</subject><subject>Male</subject><subject>Measles - blood</subject><subject>Measles - complications</subject><subject>Measles - prevention & control</subject><subject>Measles virus</subject><subject>Measles virus - genetics</subject><subject>Measles virus - isolation & purification</subject><subject>Measles-Mumps-Rubella Vaccine - adverse effects</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Polymerase Chain Reaction</subject><subject>real-time quantitative RT-PCR</subject><subject>RNA, Viral - blood</subject><subject>RNA, Viral - genetics</subject><subject>United Kingdom</subject><subject>Vaccination - adverse effects</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral diseases</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAURS0EotOBBT-AvAGJRVrbie3Msq1oS-mARCmV2FiO_dK4JE6xkynln_hHPJOBrhAbv4XPvfc9XYReULJHCWH7N91qjxFe8kdoRslCZAsi6WM0I7QQmRCU76DdGG8IIeWCsadohwrOOZN0hn4dVBG8AdzX2MIAZtBVC7gDHVuIeOXCGPE1-L4DHOH7uGGdx1Xb93Yt0uPg4uAMNo1rbQCP75oeN3oF6bF4aMAFvFx-wittjPN6cL3HdgzOX68_ceiTgYdJ3qxNXdeN3v2cyGgasGO72e_y_TP0pNZthOfbOUeXx28_H51m5x9P3h0dnGemYIxnVFOwgiyA6ooWrCxzUXFGdFVAQSEvxELUlQFhc2oqaYvCSCtrrWttaqMpz-fo9eR7G_p0cxxU56KBttUe-jEqIUtCqCD_BRlhKTgtMEdvJtCEPsYAtboNrtPhXlGi1iWqVKLalJjYl1vTserAPpDb1hLwagvoaHRbB-2Niw-cFJILLhO3P3F3roX7fyeqs-WXP9HZpEidwo-_Ch2-pZtzydXVhxN1dXhxfHb4VaqL_DdEesaM</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Afzal, M.A.</creator><creator>Ozoemena, L.C.</creator><creator>O'Hare, A.</creator><creator>Kidger, K.A.</creator><creator>Bentley, M.L.</creator><creator>Minor, P.D.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200605</creationdate><title>Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK</title><author>Afzal, M.A. ; Ozoemena, L.C. ; O'Hare, A. ; Kidger, K.A. ; Bentley, M.L. ; Minor, P.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4225-1a1ed609e1ab1428836b520ab4e41e34696fbce6d31cb7d44c7d7faafacfca153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>autism</topic><topic>Autistic Disorder - etiology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genome, Viral</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Leukocytes - virology</topic><topic>Male</topic><topic>Measles - blood</topic><topic>Measles - complications</topic><topic>Measles - prevention & control</topic><topic>Measles virus</topic><topic>Measles virus - genetics</topic><topic>Measles virus - isolation & purification</topic><topic>Measles-Mumps-Rubella Vaccine - adverse effects</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Polymerase Chain Reaction</topic><topic>real-time quantitative RT-PCR</topic><topic>RNA, Viral - blood</topic><topic>RNA, Viral - genetics</topic><topic>United Kingdom</topic><topic>Vaccination - adverse effects</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral diseases</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Afzal, M.A.</creatorcontrib><creatorcontrib>Ozoemena, L.C.</creatorcontrib><creatorcontrib>O'Hare, A.</creatorcontrib><creatorcontrib>Kidger, K.A.</creatorcontrib><creatorcontrib>Bentley, M.L.</creatorcontrib><creatorcontrib>Minor, P.D.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Afzal, M.A.</au><au>Ozoemena, L.C.</au><au>O'Hare, A.</au><au>Kidger, K.A.</au><au>Bentley, M.L.</au><au>Minor, P.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>78</volume><issue>5</issue><spage>623</spage><epage>630</epage><pages>623-630</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>Leukocyte preparations from children with documented evidence of MMR vaccination and confirmed diagnosis of autism were examined by several assays designed to target multiple regions of the measles virus genome sequence. No sample was found positive by any method. The assays applied were highly sensitive, specific and robust in nature, and were based on the amplification of measles virus RNA transcripts by real‐time quantitative RT‐PCR (QRT‐PCR) as well as by conventional RT‐PCR‐nested PCR. The assays applied were potentially able to detect measles virus RNA down to single figure copy numbers per reaction. The amount of total nucleic acid extract of leukocytes subjected to various measles virus‐specific investigations was several fold higher than minimally required of a sample where measles virus persistence is well documented. This study failed to substantiate reports of the persistence of measles virus in autistic children with development regression. J. Med. Virol. 78:623–630, 2006. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16555271</pmid><doi>10.1002/jmv.20585</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 2006-05, Vol.78 (5), p.623-630 |
| issn | 0146-6615 1096-9071 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67800160 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adolescent autism Autistic Disorder - etiology Biological and medical sciences Child Child, Preschool Female Fundamental and applied biological sciences. Psychology Genome, Viral Human viral diseases Humans Infectious diseases Leukocytes - virology Male Measles - blood Measles - complications Measles - prevention & control Measles virus Measles virus - genetics Measles virus - isolation & purification Measles-Mumps-Rubella Vaccine - adverse effects Medical sciences Microbiology Miscellaneous Polymerase Chain Reaction real-time quantitative RT-PCR RNA, Viral - blood RNA, Viral - genetics United Kingdom Vaccination - adverse effects Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral diseases Virology |
| title | Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T08%3A34%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Absence%20of%20detectable%20measles%20virus%20genome%20sequence%20in%20blood%20of%20autistic%20children%20who%20have%20had%20their%20MMR%20vaccination%20during%20the%20routine%20childhood%20immunization%20schedule%20of%20UK&rft.jtitle=Journal%20of%20medical%20virology&rft.au=Afzal,%20M.A.&rft.date=2006-05&rft.volume=78&rft.issue=5&rft.spage=623&rft.epage=630&rft.pages=623-630&rft.issn=0146-6615&rft.eissn=1096-9071&rft.coden=JMVIDB&rft_id=info:doi/10.1002/jmv.20585&rft_dat=%3Cproquest_cross%3E20252088%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20252088&rft_id=info:pmid/16555271&rfr_iscdi=true |