Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis
Istituto di Patologia Speciale e Clinica Medica Veterinaria, Universita degli Studi di Sassari, Italy. alberti@uniss.it An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria othe...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2006-01, Vol.18 (1), p.41-51 |
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| creator | Alberti, A Addis, MF Chessa, B Cubeddu, T Profiti, M Rosati, S Ruiu, A Pittau, M |
| description | Istituto di Patologia Speciale e Clinica Medica Veterinaria, Universita degli Studi di Sassari, Italy. alberti@uniss.it
An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vspA, vsp422-4, and vsp422-8 genes of the M. bovis reference strain PG45T and of the field strain 422. The use of a cross-reactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy). |
| doi_str_mv | 10.1177/104063870601800106 |
| format | Article |
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An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vspA, vsp422-4, and vsp422-8 genes of the M. bovis reference strain PG45T and of the field strain 422. The use of a cross-reactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy).</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870601800106</identifier><identifier>PMID: 16566256</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Animals ; Antigens, Bacterial - analysis ; Blotting, Western - veterinary ; Cattle ; Cattle Diseases - diagnosis ; Cattle Diseases - epidemiology ; Cattle Diseases - microbiology ; Cluster Analysis ; Disease Outbreaks - veterinary ; DNA, Bacterial - chemistry ; DNA, Bacterial - isolation & purification ; Incidence ; Keratoconjunctivitis, Infectious - diagnosis ; Keratoconjunctivitis, Infectious - epidemiology ; Keratoconjunctivitis, Infectious - microbiology ; Molecular Sequence Data ; Mycoplasma bovis - classification ; Mycoplasma bovis - genetics ; Mycoplasma bovis - immunology ; Mycoplasma Infections - diagnosis ; Mycoplasma Infections - epidemiology ; Mycoplasma Infections - microbiology ; Mycoplasma Infections - veterinary ; Phylogeny ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - veterinary ; Polymorphism, Restriction Fragment Length ; Random Amplified Polymorphic DNA Technique ; Restriction Mapping - veterinary</subject><ispartof>Journal of veterinary diagnostic investigation, 2006-01, Vol.18 (1), p.41-51</ispartof><rights>2006 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-7fdbc1b8533f87793949249ff051e6f3a0568a35e8524dff00b150dc09827b963</citedby><cites>FETCH-LOGICAL-c481t-7fdbc1b8533f87793949249ff051e6f3a0568a35e8524dff00b150dc09827b963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063870601800106$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063870601800106$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16566256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alberti, A</creatorcontrib><creatorcontrib>Addis, MF</creatorcontrib><creatorcontrib>Chessa, B</creatorcontrib><creatorcontrib>Cubeddu, T</creatorcontrib><creatorcontrib>Profiti, M</creatorcontrib><creatorcontrib>Rosati, S</creatorcontrib><creatorcontrib>Ruiu, A</creatorcontrib><creatorcontrib>Pittau, M</creatorcontrib><title>Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Istituto di Patologia Speciale e Clinica Medica Veterinaria, Universita degli Studi di Sassari, Italy. alberti@uniss.it
An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vspA, vsp422-4, and vsp422-8 genes of the M. bovis reference strain PG45T and of the field strain 422. The use of a cross-reactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy).</description><subject>Animals</subject><subject>Antigens, Bacterial - analysis</subject><subject>Blotting, Western - veterinary</subject><subject>Cattle</subject><subject>Cattle Diseases - diagnosis</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - microbiology</subject><subject>Cluster Analysis</subject><subject>Disease Outbreaks - veterinary</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>Incidence</subject><subject>Keratoconjunctivitis, Infectious - diagnosis</subject><subject>Keratoconjunctivitis, Infectious - epidemiology</subject><subject>Keratoconjunctivitis, Infectious - microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycoplasma bovis - classification</subject><subject>Mycoplasma bovis - genetics</subject><subject>Mycoplasma bovis - immunology</subject><subject>Mycoplasma Infections - diagnosis</subject><subject>Mycoplasma Infections - epidemiology</subject><subject>Mycoplasma Infections - microbiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Restriction Mapping - veterinary</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9rHCEYxqUkNH_aL9BD8JLepvHP6DjHEtI2kJBLepZ3HN11M6tbdTak9MPHZRdyCPQgysvv96gPQl8o-UZp111R0hLJVUckoYoQSuQHdEr7ljdtz-VRPVeg2REn6CznFSGCiY5-RCdUCimZkKfo332crJknSBjCWFfxCxu8wWYJCUyxyf-F4mPA0WHA9y8mbibIa8BD3PqMc0ngAzYwZx8W1cdxLkOy8LQTfHDWVHvO-MkmKNHEsJpDHW198fkTOnYwZfv5sJ-j3z9uHq9_NXcPP2-vv981plW0NJ0bB0MHJTh3qut63rc9a3vniKBWOg5ESAVcWCVYO9YxGaggoyG9Yt3QS36Ovu5zNyn-mW0ueu2zsdMEwda3aVlDFZOsgmwPmhRzTtbpTfJrSC-aEr3rXL_vvEoXh_R5WNvxTTmUXIGrPZBhYfUqzinU3_4_8nJvLP1i-eyT1bXxaaoXML3ajp4qTXVL-SuiVZja</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Alberti, A</creator><creator>Addis, MF</creator><creator>Chessa, B</creator><creator>Cubeddu, T</creator><creator>Profiti, M</creator><creator>Rosati, S</creator><creator>Ruiu, A</creator><creator>Pittau, M</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060101</creationdate><title>Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis</title><author>Alberti, A ; Addis, MF ; Chessa, B ; Cubeddu, T ; Profiti, M ; Rosati, S ; Ruiu, A ; Pittau, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-7fdbc1b8533f87793949249ff051e6f3a0568a35e8524dff00b150dc09827b963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - analysis</topic><topic>Blotting, Western - veterinary</topic><topic>Cattle</topic><topic>Cattle Diseases - diagnosis</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - microbiology</topic><topic>Cluster Analysis</topic><topic>Disease Outbreaks - veterinary</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>Incidence</topic><topic>Keratoconjunctivitis, Infectious - diagnosis</topic><topic>Keratoconjunctivitis, Infectious - epidemiology</topic><topic>Keratoconjunctivitis, Infectious - microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycoplasma bovis - classification</topic><topic>Mycoplasma bovis - genetics</topic><topic>Mycoplasma bovis - immunology</topic><topic>Mycoplasma Infections - diagnosis</topic><topic>Mycoplasma Infections - epidemiology</topic><topic>Mycoplasma Infections - microbiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Restriction Mapping - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alberti, A</creatorcontrib><creatorcontrib>Addis, MF</creatorcontrib><creatorcontrib>Chessa, B</creatorcontrib><creatorcontrib>Cubeddu, T</creatorcontrib><creatorcontrib>Profiti, M</creatorcontrib><creatorcontrib>Rosati, S</creatorcontrib><creatorcontrib>Ruiu, A</creatorcontrib><creatorcontrib>Pittau, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alberti, A</au><au>Addis, MF</au><au>Chessa, B</au><au>Cubeddu, T</au><au>Profiti, M</au><au>Rosati, S</au><au>Ruiu, A</au><au>Pittau, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>18</volume><issue>1</issue><spage>41</spage><epage>51</epage><pages>41-51</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Istituto di Patologia Speciale e Clinica Medica Veterinaria, Universita degli Studi di Sassari, Italy. alberti@uniss.it
An unusually high incidence of infectious keratoconjunctivitis followed by pneumonia and arthritis was observed in beef calves of a managed herd. No Moraxella spp. or bacteria other than Mycoplasma spp. were obtained from conjunctival and nasal swabs. A strategy was designed for characterization of bovine mycoplasmas at species and strain level on the basis of a combination of molecular tools and the immunoblotting method. The strategy made it possible to rapidly assign the bacterium responsible for this outbreak to one of the phylogenetic clusters of bovine mycoplasmas delineated in this study and then to identify it as Mycoplasma bovis. The strain, designated Sar 1, showed a 100% 16S rDNA sequence identity with two European strains (120/81 and MC3386) isolated in Germany and Ireland, respectively, and hosts a vsp gene analog to the vspA, vsp422-4, and vsp422-8 genes of the M. bovis reference strain PG45T and of the field strain 422. The use of a cross-reactive rabbit serum developed against the Mycoplasma agalactiae immunodominant antigen P48 confirmed the molecular findings. The immunological response of calves against M. bovis was also investigated. This is the first report on the occurrence of M. bovis on the Island of Sardinia (Italy).</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>16566256</pmid><doi>10.1177/104063870601800106</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1040-6387 |
| ispartof | Journal of veterinary diagnostic investigation, 2006-01, Vol.18 (1), p.41-51 |
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| language | eng |
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| source | SAGE Complete A-Z List; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Antigens, Bacterial - analysis Blotting, Western - veterinary Cattle Cattle Diseases - diagnosis Cattle Diseases - epidemiology Cattle Diseases - microbiology Cluster Analysis Disease Outbreaks - veterinary DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification Incidence Keratoconjunctivitis, Infectious - diagnosis Keratoconjunctivitis, Infectious - epidemiology Keratoconjunctivitis, Infectious - microbiology Molecular Sequence Data Mycoplasma bovis - classification Mycoplasma bovis - genetics Mycoplasma bovis - immunology Mycoplasma Infections - diagnosis Mycoplasma Infections - epidemiology Mycoplasma Infections - microbiology Mycoplasma Infections - veterinary Phylogeny Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Restriction Mapping - veterinary |
| title | Molecular and antigenic characterization of a Mycoplasma bovis strain causing an outbreak of infectious keratoconjunctivitis |
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