Combined pH/organic solvent gradient HPLC in analysis of forensic material
A combined pH/organic solvent linear gradient mode in high performance liquid chromatography (HPLC) is presented as a new approach to determination of low concentrations of ionogenic analytes in biological material. The approach consists in simultaneous development of linear gradients of pH and orga...
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| Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2005-04, Vol.37 (5), p.871-875 |
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| container_issue | 5 |
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| container_title | Journal of pharmaceutical and biomedical analysis |
| container_volume | 37 |
| creator | Wiczling, Paweł Markuszewski, Michał J. Kaliszan, Michał Galer, Katarzyna Kaliszan, Roman |
| description | A combined pH/organic solvent linear gradient mode in high performance liquid chromatography (HPLC) is presented as a new approach to determination of low concentrations of ionogenic analytes in biological material. The approach consists in simultaneous development of linear gradients of pH and organic modifier in the mobile phase. Advantages of the method are illustrated in postmortem analysis of opipramol in material from suicide victims. Very narrow peaks without tailing were obtained and several times lower limits of analyte quantitation were achieved using ultraviolet detection as compared to a standard isocratic method. The double gradient HPLC method seems to be especially valuable in case of ionogenic analytes dispersed in complex biological matrices. That is due to a high selectivity of the double gradient method and the lack of peak tailing, which is commonly observed for basic analytes chromatographed at isocratic conditions. |
| doi_str_mv | 10.1016/j.jpba.2004.06.031 |
| format | Article |
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The approach consists in simultaneous development of linear gradients of pH and organic modifier in the mobile phase. Advantages of the method are illustrated in postmortem analysis of opipramol in material from suicide victims. Very narrow peaks without tailing were obtained and several times lower limits of analyte quantitation were achieved using ultraviolet detection as compared to a standard isocratic method. The double gradient HPLC method seems to be especially valuable in case of ionogenic analytes dispersed in complex biological matrices. That is due to a high selectivity of the double gradient method and the lack of peak tailing, which is commonly observed for basic analytes chromatographed at isocratic conditions.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Female</subject><subject>Forensic analysis</subject><subject>Forensic Pathology - instrumentation</subject><subject>Forensic Pathology - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Opipramol</subject><subject>Organic Chemicals - blood</subject><subject>Organic Chemicals - chemistry</subject><subject>Organic Chemicals - urine</subject><subject>pH/organic solvent gradient HPLC</subject><subject>Pharmacology. 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Psychology</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Opipramol</topic><topic>Organic Chemicals - blood</topic><topic>Organic Chemicals - chemistry</topic><topic>Organic Chemicals - urine</topic><topic>pH/organic solvent gradient HPLC</topic><topic>Pharmacology. 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The approach consists in simultaneous development of linear gradients of pH and organic modifier in the mobile phase. Advantages of the method are illustrated in postmortem analysis of opipramol in material from suicide victims. Very narrow peaks without tailing were obtained and several times lower limits of analyte quantitation were achieved using ultraviolet detection as compared to a standard isocratic method. The double gradient HPLC method seems to be especially valuable in case of ionogenic analytes dispersed in complex biological matrices. That is due to a high selectivity of the double gradient method and the lack of peak tailing, which is commonly observed for basic analytes chromatographed at isocratic conditions.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15862660</pmid><doi>10.1016/j.jpba.2004.06.031</doi><tpages>5</tpages></addata></record> |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Female Forensic analysis Forensic Pathology - instrumentation Forensic Pathology - methods Fundamental and applied biological sciences. Psychology General pharmacology Humans Hydrogen-Ion Concentration Male Medical sciences Opipramol Organic Chemicals - blood Organic Chemicals - chemistry Organic Chemicals - urine pH/organic solvent gradient HPLC Pharmacology. Drug treatments Solvents - analysis Solvents - chemistry |
| title | Combined pH/organic solvent gradient HPLC in analysis of forensic material |
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