Involvement of Bax, Bcl-2 and Caspase 3 in Hydroxyurea- or Etoposide-induced Apoptosis of Mouse Interleukin-3-dependent Lymphoma Cells

Background: The apoptosis action induced by hydroxyurea or etoposide in interleukin 3-dependent lymphoma cells (DA-1) was studied. Materials and Methods: The conditions to study apoptosis of these cells were 17 hours of cell treatment with concentrations of 1.25 mM hydroxyurea or 100 Î¼M etoposide u...

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Veröffentlicht in:	Anticancer research 2005-03, Vol.25 (2A), p.999-1007
Hauptverfasser:	OLMOS, Gemma, PRIETO, Alfredo, HERRAEZ, Angel, TEJEDOR, M. Cristina, ALVAREZ-MON, Melchor, DIEZ, José C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antineoplastic Agents - pharmacology Antineoplastic Agents, Phytogenic - pharmacology Apoptosis - drug effects bcl-2-Associated X Protein Biological and medical sciences Caspase 3 Caspases - biosynthesis Cell Cycle - drug effects Cell Line, Tumor Culture Media Enzyme Induction - drug effects Etoposide - pharmacology Hematologic and hematopoietic diseases Hydroxyurea - pharmacology Interleukin-3 - pharmacology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma - drug therapy Lymphoma - pathology Medical sciences Mice Proto-Oncogene Proteins c-bcl-2 - biosynthesis Tumor Suppressor Protein p53 - biosynthesis Tumors
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Zusammenfassung:	Background: The apoptosis action induced by hydroxyurea or etoposide in interleukin 3-dependent lymphoma cells (DA-1) was studied. Materials and Methods: The conditions to study apoptosis of these cells were 17 hours of cell treatment with concentrations of 1.25 mM hydroxyurea or 100 Î¼M etoposide using flow cytometry, fluorometry and immunoblots techniques. Results: Time-dependent reductions of cell viability after these treatments were observed. Caspase 3 activity was highly activated in both cases. Chemical treatments or interleukin 3 withdrawal rendered level changes in Bax and Bcl-2 expression. Conclusion: These results support the implication of these factors in DA-1 cell apoptosis induced by these chemical treatments or interleukin 3 depletion.
ISSN:	0250-7005 1791-7530