Gene silencing studies in the gymnosperm species Pinus radiata

A biolistic transformation procedure was used to transform embryogenic Pinus radiata tissue with constructs containing the Zea mays UBI1 (ubiquitin)-promoter followed by the P. radiata CAD (cinnamyl alcohol dehydrogenase) cDNA in sense or anti-sense orientation or in the form of an inverted-repeat....

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Veröffentlicht in:	Plant cell reports 2005-05, Vol.24 (2), p.95-102
Hauptverfasser:	Wagner, A, Phillips, L, Narayan, R.D, Moody, J.M, Geddes, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Oxidoreductases - genetics biolistic transformation Biological and medical sciences Biotechnology cinnamyl alcohol dehydrogenase complementary DNA DNA, Complementary - genetics DNA, Plant - genetics embryo (plant) Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - genetics Gene Expression Regulation, Plant - genetics gene silencing Gene Silencing - physiology Genetic engineering Genetic technics Methods. Procedures. Technologies molecular sequence data nucleotide sequences Pinus - embryology Pinus - genetics Pinus - growth & development Pinus radiata Plant Proteins - genetics Plant Shoots - embryology Plant Shoots - genetics Plant Shoots - growth & development Plants, Genetically Modified - embryology Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Promoter Regions, Genetic - genetics RNA, Messenger - metabolism Seedlings Seeds - embryology Seeds - genetics Seeds - growth & development terminal repeat sequences Transformation, Genetic - genetics Transgenes - genetics Transgenic animals and transgenic plants Transgenic plants Ubiquitin C - genetics Zea mays
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