Cytochrome P450 biosensors: a review

Cytochrome P450 (CYP) is a large family of enzymes containing heme as the active site. Since their discovery and the elucidation of their structure, they have attracted the interest of scientist for many years, particularly due to their catalytic abilities. Since the late 1970s attempts have concent...

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Veröffentlicht in:Biosensors & bioelectronics 2005-06, Vol.20 (12), p.2408-2423
Hauptverfasser: BISTOLAS, Nikitas, WOLLENBERGER, Ulla, JUNG, Christiane, SCHELLER, Frieder W
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container_issue 12
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creator BISTOLAS, Nikitas
WOLLENBERGER, Ulla
JUNG, Christiane
SCHELLER, Frieder W
description Cytochrome P450 (CYP) is a large family of enzymes containing heme as the active site. Since their discovery and the elucidation of their structure, they have attracted the interest of scientist for many years, particularly due to their catalytic abilities. Since the late 1970s attempts have concentrated on the construction and development of electrochemical sensors. Although sensors based on mediated electron transfer have also been constructed, the direct electron transfer approach has attracted most of the interest. This has enabled the investigation of the electrochemical properties of the various isoforms of CYP. Furthermore, CYP utilized to construct biosensors for the determination of substrates important in environmental monitoring, pharmaceutical industry and clinical practice.
doi_str_mv 10.1016/j.bios.2004.11.023
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source MEDLINE; Access via ScienceDirect (Elsevier)
subjects Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Coated Materials, Biocompatible - chemistry
Cytochrome P-450 Enzyme System - analysis
Cytochrome P-450 Enzyme System - chemistry
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Equipment Design
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Surface Plasmon Resonance - instrumentation
Surface Plasmon Resonance - methods
Various methods and equipments
title Cytochrome P450 biosensors: a review
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