Enrichment for microbes living in association with plant tissues

To investigate a cultivation-independent method of enrichment for microbes living in association with plant tissues. A large quantity of leaves or seeds was enzymatically hydrolyzed, and the pellets were collected by differential centrifugation. Enzyme concentration, buffer and incubation time were...

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Veröffentlicht in:	Journal of applied microbiology 2006-04, Vol.100 (4), p.830-837
Hauptverfasser:	Jiao, J.-Y, Wang, H.-X, Zeng, Y, Shen, Y.-M
Format:	Artikel
Sprache:	eng
Schlagworte:	16S rDNA bacteria Biological and medical sciences Cell Nucleus - chemistry centrifugation clones Cloning, Molecular - methods DNA DNA, Bacterial - analysis DNA, Plant - analysis ecology endophytes Euphorbiaceae - genetics Euphorbiaceae - microbiology Fundamental and applied biological sciences. Psychology Genes, Plant - genetics genetic markers leaves Maytenus - genetics Maytenus - microbiology Maytenus hookeri metagenome metagenomics microbe enrichment Microbiology nuclear genome pellets Plant Leaves - microbiology plant tissues Plastids - genetics Plastids - microbiology polymerase chain reaction Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length restriction fragment length polymorphism ribosomal RNA RNA, Ribosomal, 16S - analysis seeds Seeds - microbiology Sequence Analysis - methods Trewia nudiflora uncultured microbes
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