Increased BNip3 and decreased mutant p53 in cisplatin-sensitive PDT-resistant HT29 cells
We have reported previously the isolation of three photodynamic therapy (PDT)-resistant human colon carcinoma HT29 cell lines that show increased expression of the Hsp27 and BNip3 protein and a decreased expression of the mutant p53 protein compared to parental HT29 cells. Since mutant p53 and incre...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2005-06, Vol.331 (2), p.648-657 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Zacal, Natalie Espiritu, Myrna Singh, Gurmit Rainbow, Andrew J. |
| description | We have reported previously the isolation of three photodynamic therapy (PDT)-resistant human colon carcinoma HT29 cell lines that show increased expression of the Hsp27 and BNip3 protein and a decreased expression of the mutant p53 protein compared to parental HT29 cells. Since mutant p53 and increased expression of Hsp27 have been associated with resistance to various chemotherapeutic agents, whereas BNip3 is a potent inducer of apoptosis, we were interested in determining whether these PDT-resistant cells were cross-resistant to other cytotoxic agents. We report here that the PDT-resistant HT29 cell lines showed a significant increase in cisplatin sensitivity and an increase in both spontaneous and cisplatin-induced apoptosis compared to parental HT29 cells. In addition, the cisplatin sensitivity of the PDT-resistant HT29 variants and several other clonal variants of HT29 cells correlated with increased BNip3 and decreased mutant p53 protein levels, but not Hsp27 protein levels. |
| doi_str_mv | 10.1016/j.bbrc.2005.03.213 |
| format | Article |
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Since mutant p53 and increased expression of Hsp27 have been associated with resistance to various chemotherapeutic agents, whereas BNip3 is a potent inducer of apoptosis, we were interested in determining whether these PDT-resistant cells were cross-resistant to other cytotoxic agents. We report here that the PDT-resistant HT29 cell lines showed a significant increase in cisplatin sensitivity and an increase in both spontaneous and cisplatin-induced apoptosis compared to parental HT29 cells. 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Since mutant p53 and increased expression of Hsp27 have been associated with resistance to various chemotherapeutic agents, whereas BNip3 is a potent inducer of apoptosis, we were interested in determining whether these PDT-resistant cells were cross-resistant to other cytotoxic agents. We report here that the PDT-resistant HT29 cell lines showed a significant increase in cisplatin sensitivity and an increase in both spontaneous and cisplatin-induced apoptosis compared to parental HT29 cells. In addition, the cisplatin sensitivity of the PDT-resistant HT29 variants and several other clonal variants of HT29 cells correlated with increased BNip3 and decreased mutant p53 protein levels, but not Hsp27 protein levels.</description><subject>Actins - metabolism</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>BNip3</subject><subject>Cell Survival - drug effects</subject><subject>Cisplatin - pharmacology</subject><subject>Cisplatin sensitivity</subject><subject>Drug Resistance, Neoplasm - genetics</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Genes, p53 - genetics</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Hsp27</subject><subject>HSP27 Heat-Shock Proteins</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Membrane Proteins - metabolism</subject><subject>Mutation - genetics</subject><subject>Neoplasm Proteins - metabolism</subject><subject>p53</subject><subject>PDT resistance</subject><subject>Photochemotherapy</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Tumor Suppressor Protein p53 - genetics</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEURYMotlb_gAuZlbsZX5ImkwE3Wj9aKOqiQnchk7yBlHY6JtOC_96prbjT1YPLuZfHIeSSQkaByptFVpbBZgxAZMAzRvkR6VMoIGUUhsekDwAyZQWd98hZjAsASoeyOCU9KpQABapP5pPaBjQRXXL_4huemNolDn-y1aY1dZs0gie-TqyPzdK0vk4j1tG3fovJ28MsDRh9_AbHM1YkFpfLeE5OKrOMeHG4A_L-9DgbjdPp6_NkdDdNLVeiTSsukDkrrauqvJSFkKUySJmyTlZYOqMkWiUc5ExhNTSMMW4ZdYaiZWUl-YBc73ebsP7YYGz1ysfdB6bG9SZqmee5UAz-BWk-LABy2oFsD9qwjjFgpZvgVyZ8agp6J14v9E683onXwHUnvitdHdY35Qrdb-VgugNu9wB2MrYeg47WY23R-YC21W7t_9r_AsMwlF4</recordid><startdate>20050603</startdate><enddate>20050603</enddate><creator>Zacal, Natalie</creator><creator>Espiritu, Myrna</creator><creator>Singh, Gurmit</creator><creator>Rainbow, Andrew J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050603</creationdate><title>Increased BNip3 and decreased mutant p53 in cisplatin-sensitive PDT-resistant HT29 cells</title><author>Zacal, Natalie ; Espiritu, Myrna ; Singh, Gurmit ; Rainbow, Andrew J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-f35e2dc6cdff7b6956b8ae128cd6febda86ec85d0728ef4a2223c21da1ec2bf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Actins - metabolism</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>BNip3</topic><topic>Cell Survival - drug effects</topic><topic>Cisplatin - pharmacology</topic><topic>Cisplatin sensitivity</topic><topic>Drug Resistance, Neoplasm - genetics</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Genes, p53 - genetics</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Hsp27</topic><topic>HSP27 Heat-Shock Proteins</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Membrane Proteins - metabolism</topic><topic>Mutation - genetics</topic><topic>Neoplasm Proteins - metabolism</topic><topic>p53</topic><topic>PDT resistance</topic><topic>Photochemotherapy</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Tumor Suppressor Protein p53 - genetics</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zacal, Natalie</creatorcontrib><creatorcontrib>Espiritu, Myrna</creatorcontrib><creatorcontrib>Singh, Gurmit</creatorcontrib><creatorcontrib>Rainbow, Andrew J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zacal, Natalie</au><au>Espiritu, Myrna</au><au>Singh, Gurmit</au><au>Rainbow, Andrew J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased BNip3 and decreased mutant p53 in cisplatin-sensitive PDT-resistant HT29 cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2005-06-03</date><risdate>2005</risdate><volume>331</volume><issue>2</issue><spage>648</spage><epage>657</epage><pages>648-657</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>We have reported previously the isolation of three photodynamic therapy (PDT)-resistant human colon carcinoma HT29 cell lines that show increased expression of the Hsp27 and BNip3 protein and a decreased expression of the mutant p53 protein compared to parental HT29 cells. Since mutant p53 and increased expression of Hsp27 have been associated with resistance to various chemotherapeutic agents, whereas BNip3 is a potent inducer of apoptosis, we were interested in determining whether these PDT-resistant cells were cross-resistant to other cytotoxic agents. We report here that the PDT-resistant HT29 cell lines showed a significant increase in cisplatin sensitivity and an increase in both spontaneous and cisplatin-induced apoptosis compared to parental HT29 cells. In addition, the cisplatin sensitivity of the PDT-resistant HT29 variants and several other clonal variants of HT29 cells correlated with increased BNip3 and decreased mutant p53 protein levels, but not Hsp27 protein levels.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15850808</pmid><doi>10.1016/j.bbrc.2005.03.213</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Actins - metabolism Apoptosis Apoptosis - drug effects BNip3 Cell Survival - drug effects Cisplatin - pharmacology Cisplatin sensitivity Drug Resistance, Neoplasm - genetics Gene Expression Regulation, Neoplastic - drug effects Genes, p53 - genetics Heat-Shock Proteins - metabolism Hsp27 HSP27 Heat-Shock Proteins HT29 Cells Humans Membrane Proteins - metabolism Mutation - genetics Neoplasm Proteins - metabolism p53 PDT resistance Photochemotherapy Proto-Oncogene Proteins - metabolism Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism |
| title | Increased BNip3 and decreased mutant p53 in cisplatin-sensitive PDT-resistant HT29 cells |
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