The integration host factor (IHF) integrates stationary‐phase and virulence gene expression in Salmonella enterica serovar Typhimurium
Summary The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization and transcriptional regulation in Gram‐negative bacteria. This heterodimeric protein is composed of the two highly homologous subunits IHFα and IHFβ. DNA microarray analysi...
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creator | Mangan, Michael W. Lucchini, Sacha Danino, Vittoria Cróinín, Tadhg Ó Hinton, Jay C. D. Dorman, Charles J. |
description | Summary
The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization and transcriptional regulation in Gram‐negative bacteria. This heterodimeric protein is composed of the two highly homologous subunits IHFα and IHFβ. DNA microarray analysis was used to define the regulon of genes subject to IHF control in Salmonella enterica serovar Typhimurium (S. Typhimurium). The transcription profile of the wild type was compared with those of mutants deficient in IHFα, IHFβ, or both IHFα and IHFβ. Our data reveal a new connection between IHF and the expression of genes required by the bacterium to undergo the physiological changes associated with the transition from exponential growth to stationary phase. When a mutant lacking IHF entered stationary phase, it displayed downregulated expression of classic stationary‐phase genes in the absence of any concomitant change in expression of the RpoS sigma factor. Purified IHF was found to bind to the regulatory regions of stationary‐phase genes indicating an auxiliary and direct role for IHF in RpoS‐dependent gene activation. Loss of IHF also had a profound influence on expression of the major virulence genes and epithelial cell invasion, indicating a role in co‐ordinating regulation of the pathogenic traits with adaptation to stationary phase. Although the three mutants showed considerable overlaps in the genes affected by the ihf lesions, the observed patterns were not identical, showing that S. Typhimurium has not one but three overlapping IHF regulons. |
doi_str_mv | 10.1111/j.1365-2958.2006.05062.x |
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The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization and transcriptional regulation in Gram‐negative bacteria. This heterodimeric protein is composed of the two highly homologous subunits IHFα and IHFβ. DNA microarray analysis was used to define the regulon of genes subject to IHF control in Salmonella enterica serovar Typhimurium (S. Typhimurium). The transcription profile of the wild type was compared with those of mutants deficient in IHFα, IHFβ, or both IHFα and IHFβ. Our data reveal a new connection between IHF and the expression of genes required by the bacterium to undergo the physiological changes associated with the transition from exponential growth to stationary phase. When a mutant lacking IHF entered stationary phase, it displayed downregulated expression of classic stationary‐phase genes in the absence of any concomitant change in expression of the RpoS sigma factor. Purified IHF was found to bind to the regulatory regions of stationary‐phase genes indicating an auxiliary and direct role for IHF in RpoS‐dependent gene activation. Loss of IHF also had a profound influence on expression of the major virulence genes and epithelial cell invasion, indicating a role in co‐ordinating regulation of the pathogenic traits with adaptation to stationary phase. Although the three mutants showed considerable overlaps in the genes affected by the ihf lesions, the observed patterns were not identical, showing that S. Typhimurium has not one but three overlapping IHF regulons.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2006.05062.x</identifier><identifier>PMID: 16553887</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Bacterial proteins ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Cells, Cultured ; Chemotaxis - genetics ; Deoxyribonucleic acid ; DNA ; E coli ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Gene Expression Regulation, Bacterial ; Genes, Bacterial - genetics ; Integration Host Factors - genetics ; Integration Host Factors - physiology ; Microbiology ; Miscellaneous ; Mutation ; Oligonucleotide Array Sequence Analysis ; Salmonella ; Salmonella enterica ; Salmonella typhimurium ; Salmonella typhimurium - genetics ; Salmonella typhimurium - pathogenicity ; Sigma Factor - metabolism ; Transcriptional Activation ; Virulence - genetics</subject><ispartof>Molecular microbiology, 2006-03, Vol.59 (6), p.1831-1847</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Mar 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5712-8b0cec22af19505f61b3ffa9548c4a14739361adefe844a0eb2e4f36a5d7f1e83</citedby><cites>FETCH-LOGICAL-c5712-8b0cec22af19505f61b3ffa9548c4a14739361adefe844a0eb2e4f36a5d7f1e83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2006.05062.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2006.05062.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17566941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16553887$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mangan, Michael W.</creatorcontrib><creatorcontrib>Lucchini, Sacha</creatorcontrib><creatorcontrib>Danino, Vittoria</creatorcontrib><creatorcontrib>Cróinín, Tadhg Ó</creatorcontrib><creatorcontrib>Hinton, Jay C. D.</creatorcontrib><creatorcontrib>Dorman, Charles J.</creatorcontrib><title>The integration host factor (IHF) integrates stationary‐phase and virulence gene expression in Salmonella enterica serovar Typhimurium</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization and transcriptional regulation in Gram‐negative bacteria. This heterodimeric protein is composed of the two highly homologous subunits IHFα and IHFβ. DNA microarray analysis was used to define the regulon of genes subject to IHF control in Salmonella enterica serovar Typhimurium (S. Typhimurium). The transcription profile of the wild type was compared with those of mutants deficient in IHFα, IHFβ, or both IHFα and IHFβ. Our data reveal a new connection between IHF and the expression of genes required by the bacterium to undergo the physiological changes associated with the transition from exponential growth to stationary phase. When a mutant lacking IHF entered stationary phase, it displayed downregulated expression of classic stationary‐phase genes in the absence of any concomitant change in expression of the RpoS sigma factor. Purified IHF was found to bind to the regulatory regions of stationary‐phase genes indicating an auxiliary and direct role for IHF in RpoS‐dependent gene activation. Loss of IHF also had a profound influence on expression of the major virulence genes and epithelial cell invasion, indicating a role in co‐ordinating regulation of the pathogenic traits with adaptation to stationary phase. Although the three mutants showed considerable overlaps in the genes affected by the ihf lesions, the observed patterns were not identical, showing that S. Typhimurium has not one but three overlapping IHF regulons.</description><subject>Animals</subject><subject>Bacterial proteins</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chemotaxis - genetics</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial - genetics</subject><subject>Integration Host Factors - genetics</subject><subject>Integration Host Factors - physiology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mutation</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella typhimurium</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - pathogenicity</subject><subject>Sigma Factor - metabolism</subject><subject>Transcriptional Activation</subject><subject>Virulence - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiMEotPCKyALCQSLBF_iSxYsUEXpSK1YMEjsLI_nuONR4gQ7KTO7LlnyjDwJycyoldiAN7Z0vvPrHH9ZhgguyHjebQrCBM9pxVVBMRYF5ljQYvsom90XHmczXHGcM0W_nWSnKW0wJgwL9jQ7IYJzppScZT8Xa0A-9HATTe_bgNZt6pEztm8jejO_vHh7X4WEUr-HTNz9vvvVrU0CZMIK3fo41BAsoBsIgGDbRUhpSvMBfTF10waoa4NgTIreGpQgtrcmosWuW_tmiH5onmVPnKkTPD_eZ9nXi4-L88v86vOn-fmHq9xySWiultiCpdQ4Mi7HnSBL5pypeKlsaUgpWcUEMStwoMrSYFhSKB0Thq-kI6DYWfb6kNvF9vsAqdeNT3YaL0A7JC2klKVU4p8gkRQzItgIvvwL3LRDDOMSmlSCU8r2aeoA2dimFMHpLvpm_EhNsJ6c6o2e1OlJnZ6c6r1TvR1bXxzzh2UDq4fGo8QReHUETLKmdtEE69MDJ7kQVUlG7v2B--Fr2P33APr6ej692B8n88Ay</recordid><startdate>200603</startdate><enddate>200603</enddate><creator>Mangan, Michael W.</creator><creator>Lucchini, Sacha</creator><creator>Danino, Vittoria</creator><creator>Cróinín, Tadhg Ó</creator><creator>Hinton, Jay C. D.</creator><creator>Dorman, Charles J.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200603</creationdate><title>The integration host factor (IHF) integrates stationary‐phase and virulence gene expression in Salmonella enterica serovar Typhimurium</title><author>Mangan, Michael W. ; Lucchini, Sacha ; Danino, Vittoria ; Cróinín, Tadhg Ó ; Hinton, Jay C. D. ; Dorman, Charles J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5712-8b0cec22af19505f61b3ffa9548c4a14739361adefe844a0eb2e4f36a5d7f1e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Bacterial proteins</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chemotaxis - genetics</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial - genetics</topic><topic>Integration Host Factors - genetics</topic><topic>Integration Host Factors - physiology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mutation</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella typhimurium</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - pathogenicity</topic><topic>Sigma Factor - metabolism</topic><topic>Transcriptional Activation</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mangan, Michael W.</creatorcontrib><creatorcontrib>Lucchini, Sacha</creatorcontrib><creatorcontrib>Danino, Vittoria</creatorcontrib><creatorcontrib>Cróinín, Tadhg Ó</creatorcontrib><creatorcontrib>Hinton, Jay C. D.</creatorcontrib><creatorcontrib>Dorman, Charles J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mangan, Michael W.</au><au>Lucchini, Sacha</au><au>Danino, Vittoria</au><au>Cróinín, Tadhg Ó</au><au>Hinton, Jay C. D.</au><au>Dorman, Charles J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The integration host factor (IHF) integrates stationary‐phase and virulence gene expression in Salmonella enterica serovar Typhimurium</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2006-03</date><risdate>2006</risdate><volume>59</volume><issue>6</issue><spage>1831</spage><epage>1847</epage><pages>1831-1847</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
The integration host factor (IHF) is a DNA‐binding and ‐bending protein with roles in local DNA structural organization and transcriptional regulation in Gram‐negative bacteria. This heterodimeric protein is composed of the two highly homologous subunits IHFα and IHFβ. DNA microarray analysis was used to define the regulon of genes subject to IHF control in Salmonella enterica serovar Typhimurium (S. Typhimurium). The transcription profile of the wild type was compared with those of mutants deficient in IHFα, IHFβ, or both IHFα and IHFβ. Our data reveal a new connection between IHF and the expression of genes required by the bacterium to undergo the physiological changes associated with the transition from exponential growth to stationary phase. When a mutant lacking IHF entered stationary phase, it displayed downregulated expression of classic stationary‐phase genes in the absence of any concomitant change in expression of the RpoS sigma factor. Purified IHF was found to bind to the regulatory regions of stationary‐phase genes indicating an auxiliary and direct role for IHF in RpoS‐dependent gene activation. Loss of IHF also had a profound influence on expression of the major virulence genes and epithelial cell invasion, indicating a role in co‐ordinating regulation of the pathogenic traits with adaptation to stationary phase. Although the three mutants showed considerable overlaps in the genes affected by the ihf lesions, the observed patterns were not identical, showing that S. Typhimurium has not one but three overlapping IHF regulons.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>16553887</pmid><doi>10.1111/j.1365-2958.2006.05062.x</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Bacterial proteins Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Cells, Cultured Chemotaxis - genetics Deoxyribonucleic acid DNA E coli Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation, Bacterial Genes, Bacterial - genetics Integration Host Factors - genetics Integration Host Factors - physiology Microbiology Miscellaneous Mutation Oligonucleotide Array Sequence Analysis Salmonella Salmonella enterica Salmonella typhimurium Salmonella typhimurium - genetics Salmonella typhimurium - pathogenicity Sigma Factor - metabolism Transcriptional Activation Virulence - genetics |
title | The integration host factor (IHF) integrates stationary‐phase and virulence gene expression in Salmonella enterica serovar Typhimurium |
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