Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung
Objective: To investigate whether surfactant pretreatment provides lung protection in an animal model of lung ischemia-reperfusion injury (LIRI). Methods: Male Sprague–Dawley rats (n=100) were randomised to receive intratracheally administered surfactant or no pretreatment. One hour thereafter, anim...
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Veröffentlicht in: | European journal of cardio-thoracic surgery 2005-05, Vol.27 (5), p.774-782 |
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description | Objective: To investigate whether surfactant pretreatment provides lung protection in an animal model of lung ischemia-reperfusion injury (LIRI). Methods: Male Sprague–Dawley rats (n=100) were randomised to receive intratracheally administered surfactant or no pretreatment. One hour thereafter, animals underwent 120min of warm ischemia of the left lung, or were sham-operated. A third group served as healthy untreated controls. Animals were killed on day 1, 3 or 7. Blood gas values were measured and lung compliance was recorded. Broncho-alveolar lavage fluid (BALf) was obtained to assess the amount of alveolar protein, the ratio of small to large aggregate surfactant phospholipids (SA/LA ratio), and leukocyte infiltration (granulocytes, macrophages and lymphocytes, measured by Flow Cytometry). Results: LIRI resulted in a mortality rate of 17% and significantly decreased lung compliance and PaO2 (day 1 and 3 P≪0.001, day 7 P≪0.05) as compared to sham-operated and healthy controls. On day 1 more protein was present in the alveoli of ischemic lungs (P≪0.001) than in sham-operated and healthy controls. Furthermore, LIRI resulted in an increased SA/LA ratio in the left lung on day 1 (P≪0.05) and caused infiltration of granulocytes (day 1, 3 and 7 (P≪0.01)), macrophages (day 3 (P≪0.05) and 7 (P≪0.01) and lymphocytes (day 3 and 7 (P≪0.01)) in the BALf as compared to sham-operated and healthy controls. Surfactant pretreatment improved survival, lung compliance (day 3 P≪0.001) and PaO2 (day 1, 3 (P≪0.01 and 7 (P≪0.05)). It also reduced protein leakage (P≪0.05) and prevented an increase in the SA/LA ratio (P≪0.01). Although the number of macrophages and granulocytes in the BALF was increased on day 1 and 3 (P≪0.01) after surfactant pretreatment as compared to all other groups, the number of lymphocytes was reduced on day 3 (P≪0.05). Conclusions: The present study shows that surfactant pretreatment enhances recovery of lung function and lung mechanics after LIRI, resulting in normal parameters from day 3 onwards. Surfactant pretreatment in this LIRI model may provide useful information to improve donor lung function after lung transplantation. |
doi_str_mv | 10.1016/j.ejcts.2004.12.034 |
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Methods: Male Sprague–Dawley rats (n=100) were randomised to receive intratracheally administered surfactant or no pretreatment. One hour thereafter, animals underwent 120min of warm ischemia of the left lung, or were sham-operated. A third group served as healthy untreated controls. Animals were killed on day 1, 3 or 7. Blood gas values were measured and lung compliance was recorded. Broncho-alveolar lavage fluid (BALf) was obtained to assess the amount of alveolar protein, the ratio of small to large aggregate surfactant phospholipids (SA/LA ratio), and leukocyte infiltration (granulocytes, macrophages and lymphocytes, measured by Flow Cytometry). Results: LIRI resulted in a mortality rate of 17% and significantly decreased lung compliance and PaO2 (day 1 and 3 P≪0.001, day 7 P≪0.05) as compared to sham-operated and healthy controls. On day 1 more protein was present in the alveoli of ischemic lungs (P≪0.001) than in sham-operated and healthy controls. Furthermore, LIRI resulted in an increased SA/LA ratio in the left lung on day 1 (P≪0.05) and caused infiltration of granulocytes (day 1, 3 and 7 (P≪0.01)), macrophages (day 3 (P≪0.05) and 7 (P≪0.01) and lymphocytes (day 3 and 7 (P≪0.01)) in the BALf as compared to sham-operated and healthy controls. Surfactant pretreatment improved survival, lung compliance (day 3 P≪0.001) and PaO2 (day 1, 3 (P≪0.01 and 7 (P≪0.05)). It also reduced protein leakage (P≪0.05) and prevented an increase in the SA/LA ratio (P≪0.01). Although the number of macrophages and granulocytes in the BALF was increased on day 1 and 3 (P≪0.01) after surfactant pretreatment as compared to all other groups, the number of lymphocytes was reduced on day 3 (P≪0.05). Conclusions: The present study shows that surfactant pretreatment enhances recovery of lung function and lung mechanics after LIRI, resulting in normal parameters from day 3 onwards. Surfactant pretreatment in this LIRI model may provide useful information to improve donor lung function after lung transplantation.</description><identifier>ISSN: 1010-7940</identifier><identifier>EISSN: 1873-734X</identifier><identifier>DOI: 10.1016/j.ejcts.2004.12.034</identifier><identifier>PMID: 15848313</identifier><language>eng</language><publisher>Germany: Elsevier Science B.V</publisher><subject>Animal models ; Animals ; Bronchoalveolar Lavage Fluid - chemistry ; Flow Cytometry ; Ischemia-reperfusion injury ; Lung ; Lung - drug effects ; Lung - physiopathology ; Lung Compliance ; Male ; Models, Animal ; Phospholipids - analysis ; Proteins - analysis ; Pulmonary surfactant ; Pulmonary Surfactants - therapeutic use ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury - prevention & control</subject><ispartof>European journal of cardio-thoracic surgery, 2005-05, Vol.27 (5), p.774-782</ispartof><rights>2005 Elsevier B.V. 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-bcbbecc0f5253d06c13db35b54ccef6328235791fc6e726bdf6d9cfa6aac5453</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15848313$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Kaaij, Niels P.</creatorcontrib><creatorcontrib>Haitsma, Jack J.</creatorcontrib><creatorcontrib>Kluin, Jolanda</creatorcontrib><creatorcontrib>Lambrecht, Bart N.</creatorcontrib><creatorcontrib>Lachmann, Burkhard</creatorcontrib><creatorcontrib>de Bruin, Ron W.F.</creatorcontrib><creatorcontrib>Bogers, Ad J.J.C.</creatorcontrib><title>Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung</title><title>European journal of cardio-thoracic surgery</title><addtitle>Eur J Cardiothorac Surg</addtitle><addtitle>Eur J Cardiothorac Surg</addtitle><description>Objective: To investigate whether surfactant pretreatment provides lung protection in an animal model of lung ischemia-reperfusion injury (LIRI). Methods: Male Sprague–Dawley rats (n=100) were randomised to receive intratracheally administered surfactant or no pretreatment. One hour thereafter, animals underwent 120min of warm ischemia of the left lung, or were sham-operated. A third group served as healthy untreated controls. Animals were killed on day 1, 3 or 7. Blood gas values were measured and lung compliance was recorded. Broncho-alveolar lavage fluid (BALf) was obtained to assess the amount of alveolar protein, the ratio of small to large aggregate surfactant phospholipids (SA/LA ratio), and leukocyte infiltration (granulocytes, macrophages and lymphocytes, measured by Flow Cytometry). Results: LIRI resulted in a mortality rate of 17% and significantly decreased lung compliance and PaO2 (day 1 and 3 P≪0.001, day 7 P≪0.05) as compared to sham-operated and healthy controls. On day 1 more protein was present in the alveoli of ischemic lungs (P≪0.001) than in sham-operated and healthy controls. Furthermore, LIRI resulted in an increased SA/LA ratio in the left lung on day 1 (P≪0.05) and caused infiltration of granulocytes (day 1, 3 and 7 (P≪0.01)), macrophages (day 3 (P≪0.05) and 7 (P≪0.01) and lymphocytes (day 3 and 7 (P≪0.01)) in the BALf as compared to sham-operated and healthy controls. Surfactant pretreatment improved survival, lung compliance (day 3 P≪0.001) and PaO2 (day 1, 3 (P≪0.01 and 7 (P≪0.05)). It also reduced protein leakage (P≪0.05) and prevented an increase in the SA/LA ratio (P≪0.01). Although the number of macrophages and granulocytes in the BALF was increased on day 1 and 3 (P≪0.01) after surfactant pretreatment as compared to all other groups, the number of lymphocytes was reduced on day 3 (P≪0.05). Conclusions: The present study shows that surfactant pretreatment enhances recovery of lung function and lung mechanics after LIRI, resulting in normal parameters from day 3 onwards. Surfactant pretreatment in this LIRI model may provide useful information to improve donor lung function after lung transplantation.</description><subject>Animal models</subject><subject>Animals</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Flow Cytometry</subject><subject>Ischemia-reperfusion injury</subject><subject>Lung</subject><subject>Lung - drug effects</subject><subject>Lung - physiopathology</subject><subject>Lung Compliance</subject><subject>Male</subject><subject>Models, Animal</subject><subject>Phospholipids - analysis</subject><subject>Proteins - analysis</subject><subject>Pulmonary surfactant</subject><subject>Pulmonary Surfactants - therapeutic use</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reperfusion Injury - prevention & control</subject><issn>1010-7940</issn><issn>1873-734X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLxDAUhYMovn-BIF25a02aV2epg-OIggtFBjchTW-0tS-TFPTfG51Bt65yA985HD6ETgjOCCbivMmgMcFnOcYsI3mGKdtC-6SQNJWUrbbjjQlO5YzhPXTgfYMxFjSXu2iP8IIVlNB9dPswOatN0H1IRgfBgQ4dxI_uoK0HpwP4pPbmFbpapw5GcHby9dAndd9M7jMZbBJeIWmn_uUI7VjdejjevIfocXH1OF-md_fXN_OLu9QwgUNamrIEY7DlOacVFobQqqS85MwYsHFhkVMuZ8QaATIXZWVFNTNWC60NZ5weorN17eiG9wl8UF0cCG2rexgmr4SUYlYQEkG6Bo0bvHdg1ejqTrtPRbD6Vqga9aNQfStUJFdRYUydbuqnsoPqL7NxFoFsDQzT-M_GdB2ofYCP34h2b3ErlVwtV89KzNniaZmv1CX9AjeHjtQ</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>van der Kaaij, Niels P.</creator><creator>Haitsma, Jack J.</creator><creator>Kluin, Jolanda</creator><creator>Lambrecht, Bart N.</creator><creator>Lachmann, Burkhard</creator><creator>de Bruin, Ron W.F.</creator><creator>Bogers, Ad J.J.C.</creator><general>Elsevier Science B.V</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung</title><author>van der Kaaij, Niels P. ; Haitsma, Jack J. ; Kluin, Jolanda ; Lambrecht, Bart N. ; Lachmann, Burkhard ; de Bruin, Ron W.F. ; Bogers, Ad J.J.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-bcbbecc0f5253d06c13db35b54ccef6328235791fc6e726bdf6d9cfa6aac5453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Flow Cytometry</topic><topic>Ischemia-reperfusion injury</topic><topic>Lung</topic><topic>Lung - drug effects</topic><topic>Lung - physiopathology</topic><topic>Lung Compliance</topic><topic>Male</topic><topic>Models, Animal</topic><topic>Phospholipids - analysis</topic><topic>Proteins - analysis</topic><topic>Pulmonary surfactant</topic><topic>Pulmonary Surfactants - therapeutic use</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reperfusion Injury - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Kaaij, Niels P.</creatorcontrib><creatorcontrib>Haitsma, Jack J.</creatorcontrib><creatorcontrib>Kluin, Jolanda</creatorcontrib><creatorcontrib>Lambrecht, Bart N.</creatorcontrib><creatorcontrib>Lachmann, Burkhard</creatorcontrib><creatorcontrib>de Bruin, Ron W.F.</creatorcontrib><creatorcontrib>Bogers, Ad J.J.C.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of cardio-thoracic surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Kaaij, Niels P.</au><au>Haitsma, Jack J.</au><au>Kluin, Jolanda</au><au>Lambrecht, Bart N.</au><au>Lachmann, Burkhard</au><au>de Bruin, Ron W.F.</au><au>Bogers, Ad J.J.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung</atitle><jtitle>European journal of cardio-thoracic surgery</jtitle><stitle>Eur J Cardiothorac Surg</stitle><addtitle>Eur J Cardiothorac Surg</addtitle><date>2005-05</date><risdate>2005</risdate><volume>27</volume><issue>5</issue><spage>774</spage><epage>782</epage><pages>774-782</pages><issn>1010-7940</issn><eissn>1873-734X</eissn><abstract>Objective: To investigate whether surfactant pretreatment provides lung protection in an animal model of lung ischemia-reperfusion injury (LIRI). Methods: Male Sprague–Dawley rats (n=100) were randomised to receive intratracheally administered surfactant or no pretreatment. One hour thereafter, animals underwent 120min of warm ischemia of the left lung, or were sham-operated. A third group served as healthy untreated controls. Animals were killed on day 1, 3 or 7. Blood gas values were measured and lung compliance was recorded. Broncho-alveolar lavage fluid (BALf) was obtained to assess the amount of alveolar protein, the ratio of small to large aggregate surfactant phospholipids (SA/LA ratio), and leukocyte infiltration (granulocytes, macrophages and lymphocytes, measured by Flow Cytometry). Results: LIRI resulted in a mortality rate of 17% and significantly decreased lung compliance and PaO2 (day 1 and 3 P≪0.001, day 7 P≪0.05) as compared to sham-operated and healthy controls. On day 1 more protein was present in the alveoli of ischemic lungs (P≪0.001) than in sham-operated and healthy controls. Furthermore, LIRI resulted in an increased SA/LA ratio in the left lung on day 1 (P≪0.05) and caused infiltration of granulocytes (day 1, 3 and 7 (P≪0.01)), macrophages (day 3 (P≪0.05) and 7 (P≪0.01) and lymphocytes (day 3 and 7 (P≪0.01)) in the BALf as compared to sham-operated and healthy controls. Surfactant pretreatment improved survival, lung compliance (day 3 P≪0.001) and PaO2 (day 1, 3 (P≪0.01 and 7 (P≪0.05)). It also reduced protein leakage (P≪0.05) and prevented an increase in the SA/LA ratio (P≪0.01). Although the number of macrophages and granulocytes in the BALF was increased on day 1 and 3 (P≪0.01) after surfactant pretreatment as compared to all other groups, the number of lymphocytes was reduced on day 3 (P≪0.05). Conclusions: The present study shows that surfactant pretreatment enhances recovery of lung function and lung mechanics after LIRI, resulting in normal parameters from day 3 onwards. Surfactant pretreatment in this LIRI model may provide useful information to improve donor lung function after lung transplantation.</abstract><cop>Germany</cop><pub>Elsevier Science B.V</pub><pmid>15848313</pmid><doi>10.1016/j.ejcts.2004.12.034</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animal models Animals Bronchoalveolar Lavage Fluid - chemistry Flow Cytometry Ischemia-reperfusion injury Lung Lung - drug effects Lung - physiopathology Lung Compliance Male Models, Animal Phospholipids - analysis Proteins - analysis Pulmonary surfactant Pulmonary Surfactants - therapeutic use Random Allocation Rats Rats, Sprague-Dawley Reperfusion Injury - prevention & control |
title | Surfactant pretreatment ameliorates ischemia-reperfusion injury of the lung |
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