K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers
Hippocampal mossy fibers, which are the axons of dentate granule cells, are continuously generated owing to adult neurogenesis of granule cells. They extend exclusively into the stratum lucidum, a proximal layer of the CA3 pyramidal cells. We visualized the mossy fiber tracts by Timm histochemical s...
Gespeichert in:
| Veröffentlicht in: | Neuroreport 2006-04, Vol.17 (5), p.481-486 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 486 |
|---|---|
| container_issue | 5 |
| container_start_page | 481 |
| container_title | Neuroreport |
| container_volume | 17 |
| creator | Tamura, Makoto Koyama, Ryuta Ikegaya, Yuji Matsuki, Norio Yamada, Maki K |
| description | Hippocampal mossy fibers, which are the axons of dentate granule cells, are continuously generated owing to adult neurogenesis of granule cells. They extend exclusively into the stratum lucidum, a proximal layer of the CA3 pyramidal cells. We visualized the mossy fiber tracts by Timm histochemical staining and DiI labeling in the cultured hippocampal slices from newborn rats. The fibers were abnormally expanded when the slices were cultured in the presence of K252a, an inhibitor of the neurotrophin receptor Trk. Similar defasciculation was observed with an inhibitor of MEK, which is one of the signaling molecules downstream of Trk. This study suggests for the first time that Trk and the MEK pathway are required for mossy fiber pathfinding. |
| doi_str_mv | 10.1097/01.wnr.0000208997.23448.ea |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67767508</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67767508</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4896-b2bc50d36e7b53b388026e2ade348941471807d071d6a3ca99299e2475381c293</originalsourceid><addsrcrecordid>eNqFkEtv1DAQgC0EotvCX0AREpyadPy2uaEKStWVuBSJm-U4DjHNCzvRqv--3oe0R-ZiaeYbz8yH0EcMFQYtbwBXuzFWkIOA0lpWhDKmKm9foQ1mkpacq9-v0QY01yXTgl-gy5T-Zl4DVm_RBRacUYXxBm0fCCf2urBjEcYu1GGZYjG1xWN8ui6akJY11qmY7dK1YWzC-Gdf7MI8T84Os-2LYUrpuWhD7WN6h960tk_-_em9Qr--f3u8_VFuf97d337dlo4pLcqa1I5DQ4WXNac1VQqI8MQ2nuY6yxdgBbIBiRthqbNaE609YZLnnR3R9Ap9Pv47x-nf6tNihpCc73s7-mlNRkgpJAf1X5AAF4ILyOCXI-hivif61swxDDY-GwxmL90ANlm6OUs3B-nG29z84TRlrQffnFtPljPw6QTY5GzfRju6kM6cFByTw7rsyO2mfsk-n_p156PpvO2X7jAaSy5KAiCAAYVynxL0BTk0mXI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20566560</pqid></control><display><type>article</type><title>K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers</title><source>Journals@Ovid Ovid Autoload</source><source>MEDLINE</source><creator>Tamura, Makoto ; Koyama, Ryuta ; Ikegaya, Yuji ; Matsuki, Norio ; Yamada, Maki K</creator><creatorcontrib>Tamura, Makoto ; Koyama, Ryuta ; Ikegaya, Yuji ; Matsuki, Norio ; Yamada, Maki K</creatorcontrib><description>Hippocampal mossy fibers, which are the axons of dentate granule cells, are continuously generated owing to adult neurogenesis of granule cells. They extend exclusively into the stratum lucidum, a proximal layer of the CA3 pyramidal cells. We visualized the mossy fiber tracts by Timm histochemical staining and DiI labeling in the cultured hippocampal slices from newborn rats. The fibers were abnormally expanded when the slices were cultured in the presence of K252a, an inhibitor of the neurotrophin receptor Trk. Similar defasciculation was observed with an inhibitor of MEK, which is one of the signaling molecules downstream of Trk. This study suggests for the first time that Trk and the MEK pathway are required for mossy fiber pathfinding.</description><identifier>ISSN: 0959-4965</identifier><identifier>EISSN: 1473-558X</identifier><identifier>DOI: 10.1097/01.wnr.0000208997.23448.ea</identifier><identifier>PMID: 16543811</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins, Inc</publisher><subject>Animals ; Biological and medical sciences ; Brain-Derived Neurotrophic Factor - metabolism ; Carbazoles - pharmacology ; Carbocyanines ; Cell Death - drug effects ; Cells, Cultured ; Development. Senescence. Regeneration. Transplantation ; Enzyme Inhibitors - pharmacology ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Hippocampus - cytology ; Hippocampus - drug effects ; Hippocampus - growth & development ; Histocytochemistry ; Indole Alkaloids ; MAP Kinase Kinase Kinases - physiology ; Mossy Fibers, Hippocampal - drug effects ; Mossy Fibers, Hippocampal - growth & development ; Nerve Growth Factors - metabolism ; Nerve Regeneration - physiology ; Organ Culture Techniques ; Rats ; Rats, Wistar ; Receptor, trkA - antagonists & inhibitors ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroreport, 2006-04, Vol.17 (5), p.481-486</ispartof><rights>2006 Lippincott Williams & Wilkins, Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4896-b2bc50d36e7b53b388026e2ade348941471807d071d6a3ca99299e2475381c293</citedby><cites>FETCH-LOGICAL-c4896-b2bc50d36e7b53b388026e2ade348941471807d071d6a3ca99299e2475381c293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17651208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16543811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamura, Makoto</creatorcontrib><creatorcontrib>Koyama, Ryuta</creatorcontrib><creatorcontrib>Ikegaya, Yuji</creatorcontrib><creatorcontrib>Matsuki, Norio</creatorcontrib><creatorcontrib>Yamada, Maki K</creatorcontrib><title>K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers</title><title>Neuroreport</title><addtitle>Neuroreport</addtitle><description>Hippocampal mossy fibers, which are the axons of dentate granule cells, are continuously generated owing to adult neurogenesis of granule cells. They extend exclusively into the stratum lucidum, a proximal layer of the CA3 pyramidal cells. We visualized the mossy fiber tracts by Timm histochemical staining and DiI labeling in the cultured hippocampal slices from newborn rats. The fibers were abnormally expanded when the slices were cultured in the presence of K252a, an inhibitor of the neurotrophin receptor Trk. Similar defasciculation was observed with an inhibitor of MEK, which is one of the signaling molecules downstream of Trk. This study suggests for the first time that Trk and the MEK pathway are required for mossy fiber pathfinding.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain-Derived Neurotrophic Factor - metabolism</subject><subject>Carbazoles - pharmacology</subject><subject>Carbocyanines</subject><subject>Cell Death - drug effects</subject><subject>Cells, Cultured</subject><subject>Development. Senescence. Regeneration. Transplantation</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - growth & development</subject><subject>Histocytochemistry</subject><subject>Indole Alkaloids</subject><subject>MAP Kinase Kinase Kinases - physiology</subject><subject>Mossy Fibers, Hippocampal - drug effects</subject><subject>Mossy Fibers, Hippocampal - growth & development</subject><subject>Nerve Growth Factors - metabolism</subject><subject>Nerve Regeneration - physiology</subject><subject>Organ Culture Techniques</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptor, trkA - antagonists & inhibitors</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0959-4965</issn><issn>1473-558X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgC0EotvCX0AREpyadPy2uaEKStWVuBSJm-U4DjHNCzvRqv--3oe0R-ZiaeYbz8yH0EcMFQYtbwBXuzFWkIOA0lpWhDKmKm9foQ1mkpacq9-v0QY01yXTgl-gy5T-Zl4DVm_RBRacUYXxBm0fCCf2urBjEcYu1GGZYjG1xWN8ui6akJY11qmY7dK1YWzC-Gdf7MI8T84Os-2LYUrpuWhD7WN6h960tk_-_em9Qr--f3u8_VFuf97d337dlo4pLcqa1I5DQ4WXNac1VQqI8MQ2nuY6yxdgBbIBiRthqbNaE609YZLnnR3R9Ap9Pv47x-nf6tNihpCc73s7-mlNRkgpJAf1X5AAF4ILyOCXI-hivif61swxDDY-GwxmL90ANlm6OUs3B-nG29z84TRlrQffnFtPljPw6QTY5GzfRju6kM6cFByTw7rsyO2mfsk-n_p156PpvO2X7jAaSy5KAiCAAYVynxL0BTk0mXI</recordid><startdate>20060403</startdate><enddate>20060403</enddate><creator>Tamura, Makoto</creator><creator>Koyama, Ryuta</creator><creator>Ikegaya, Yuji</creator><creator>Matsuki, Norio</creator><creator>Yamada, Maki K</creator><general>Lippincott Williams & Wilkins, Inc</general><general>Lippincott Williams and Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20060403</creationdate><title>K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers</title><author>Tamura, Makoto ; Koyama, Ryuta ; Ikegaya, Yuji ; Matsuki, Norio ; Yamada, Maki K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4896-b2bc50d36e7b53b388026e2ade348941471807d071d6a3ca99299e2475381c293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain-Derived Neurotrophic Factor - metabolism</topic><topic>Carbazoles - pharmacology</topic><topic>Carbocyanines</topic><topic>Cell Death - drug effects</topic><topic>Cells, Cultured</topic><topic>Development. Senescence. Regeneration. Transplantation</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - growth & development</topic><topic>Histocytochemistry</topic><topic>Indole Alkaloids</topic><topic>MAP Kinase Kinase Kinases - physiology</topic><topic>Mossy Fibers, Hippocampal - drug effects</topic><topic>Mossy Fibers, Hippocampal - growth & development</topic><topic>Nerve Growth Factors - metabolism</topic><topic>Nerve Regeneration - physiology</topic><topic>Organ Culture Techniques</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptor, trkA - antagonists & inhibitors</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamura, Makoto</creatorcontrib><creatorcontrib>Koyama, Ryuta</creatorcontrib><creatorcontrib>Ikegaya, Yuji</creatorcontrib><creatorcontrib>Matsuki, Norio</creatorcontrib><creatorcontrib>Yamada, Maki K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroreport</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamura, Makoto</au><au>Koyama, Ryuta</au><au>Ikegaya, Yuji</au><au>Matsuki, Norio</au><au>Yamada, Maki K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers</atitle><jtitle>Neuroreport</jtitle><addtitle>Neuroreport</addtitle><date>2006-04-03</date><risdate>2006</risdate><volume>17</volume><issue>5</issue><spage>481</spage><epage>486</epage><pages>481-486</pages><issn>0959-4965</issn><eissn>1473-558X</eissn><abstract>Hippocampal mossy fibers, which are the axons of dentate granule cells, are continuously generated owing to adult neurogenesis of granule cells. They extend exclusively into the stratum lucidum, a proximal layer of the CA3 pyramidal cells. We visualized the mossy fiber tracts by Timm histochemical staining and DiI labeling in the cultured hippocampal slices from newborn rats. The fibers were abnormally expanded when the slices were cultured in the presence of K252a, an inhibitor of the neurotrophin receptor Trk. Similar defasciculation was observed with an inhibitor of MEK, which is one of the signaling molecules downstream of Trk. This study suggests for the first time that Trk and the MEK pathway are required for mossy fiber pathfinding.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins, Inc</pub><pmid>16543811</pmid><doi>10.1097/01.wnr.0000208997.23448.ea</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0959-4965 |
| ispartof | Neuroreport, 2006-04, Vol.17 (5), p.481-486 |
| issn | 0959-4965 1473-558X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67767508 |
| source | Journals@Ovid Ovid Autoload; MEDLINE |
| subjects | Animals Biological and medical sciences Brain-Derived Neurotrophic Factor - metabolism Carbazoles - pharmacology Carbocyanines Cell Death - drug effects Cells, Cultured Development. Senescence. Regeneration. Transplantation Enzyme Inhibitors - pharmacology Fluorescent Dyes Fundamental and applied biological sciences. Psychology Hippocampus - cytology Hippocampus - drug effects Hippocampus - growth & development Histocytochemistry Indole Alkaloids MAP Kinase Kinase Kinases - physiology Mossy Fibers, Hippocampal - drug effects Mossy Fibers, Hippocampal - growth & development Nerve Growth Factors - metabolism Nerve Regeneration - physiology Organ Culture Techniques Rats Rats, Wistar Receptor, trkA - antagonists & inhibitors Vertebrates: nervous system and sense organs |
| title | K252a, an inhibitor of Trk, disturbs pathfinding of hippocampal mossy fibers |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T20%3A26%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=K252a,%20an%20inhibitor%20of%20Trk,%20disturbs%20pathfinding%20of%20hippocampal%20mossy%20fibers&rft.jtitle=Neuroreport&rft.au=Tamura,%20Makoto&rft.date=2006-04-03&rft.volume=17&rft.issue=5&rft.spage=481&rft.epage=486&rft.pages=481-486&rft.issn=0959-4965&rft.eissn=1473-558X&rft_id=info:doi/10.1097/01.wnr.0000208997.23448.ea&rft_dat=%3Cproquest_cross%3E67767508%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20566560&rft_id=info:pmid/16543811&rfr_iscdi=true |