Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size
A mesoporous molecular sieve silicate, SBA-15, with three pore sizes (38.1 Å, 77.3 Å, and 240 Å) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol–gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been...
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| Veröffentlicht in: | Journal of Chromatography A 2005-03, Vol.1069 (1), p.119-126 |
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| container_title | Journal of Chromatography A |
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| creator | Katiyar, Amit Ji, Lei Smirniotis, Panagiotis Pinto, Neville G. |
| description | A mesoporous molecular sieve silicate, SBA-15, with three pore sizes (38.1
Å, 77.3
Å, and 240
Å) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol–gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been described. The adsorption of proteins on these crystalline, ordered, materials has been studied. The kinetics of adsorption and equilibrium capacity have been probed with three proteins of different dimensions. The effects of electrostatic interactions and protein size are illustrated. It has been shown that SBA-15 materials can be tailored to show size selectivity for proteins, and very high capacities (450
mg/g) can be obtained. Furthermore, the rates of adsorption are shown to be dependent on the pore size, protein structure and solution pH. |
| doi_str_mv | 10.1016/j.chroma.2004.10.077 |
| format | Article |
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Å, 77.3
Å, and 240
Å) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol–gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been described. The adsorption of proteins on these crystalline, ordered, materials has been studied. The kinetics of adsorption and equilibrium capacity have been probed with three proteins of different dimensions. The effects of electrostatic interactions and protein size are illustrated. It has been shown that SBA-15 materials can be tailored to show size selectivity for proteins, and very high capacities (450
mg/g) can be obtained. Furthermore, the rates of adsorption are shown to be dependent on the pore size, protein structure and solution pH.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2004.10.077</identifier><identifier>PMID: 15844490</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adsorption ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Hydrogen-Ion Concentration ; Kinetics ; Mesoporous ; Other chromatographic methods ; Proteins ; Proteins - chemistry ; SBA-15 ; Silicates - chemistry ; Size-selective ; Static Electricity ; X-Ray Diffraction</subject><ispartof>Journal of Chromatography A, 2005-03, Vol.1069 (1), p.119-126</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-6f9058ad879b374d90c54707a47642794e86c5f577edfbd8ffa23849deafd4163</citedby><cites>FETCH-LOGICAL-c456t-6f9058ad879b374d90c54707a47642794e86c5f577edfbd8ffa23849deafd4163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2004.10.077$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,778,782,787,788,3539,23913,23914,25123,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16662288$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15844490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katiyar, Amit</creatorcontrib><creatorcontrib>Ji, Lei</creatorcontrib><creatorcontrib>Smirniotis, Panagiotis</creatorcontrib><creatorcontrib>Pinto, Neville G.</creatorcontrib><title>Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A mesoporous molecular sieve silicate, SBA-15, with three pore sizes (38.1
Å, 77.3
Å, and 240
Å) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol–gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been described. The adsorption of proteins on these crystalline, ordered, materials has been studied. The kinetics of adsorption and equilibrium capacity have been probed with three proteins of different dimensions. The effects of electrostatic interactions and protein size are illustrated. It has been shown that SBA-15 materials can be tailored to show size selectivity for proteins, and very high capacities (450
mg/g) can be obtained. Furthermore, the rates of adsorption are shown to be dependent on the pore size, protein structure and solution pH.</description><subject>Adsorption</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kinetics</subject><subject>Mesoporous</subject><subject>Other chromatographic methods</subject><subject>Proteins</subject><subject>Proteins - chemistry</subject><subject>SBA-15</subject><subject>Silicates - chemistry</subject><subject>Size-selective</subject><subject>Static Electricity</subject><subject>X-Ray Diffraction</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKAzEQhnNQbK2-gUguetuabLPJrgehFrVCQUE9hzSZ0JTdzZpsC_r0prTQmzBkYPj-YfIhdEXJmBLK79ZjvQq-UeOcEJZGYyLECRoSktOs4mIyQOcxrgmhgoj8DA1oUTLGKjJE6j34HlyLlYk-dL3zLU7VrwA3EH3ng99E3Pga9KZWAUcHW0hv7bTqAX88TjNa3GOwFnQfsbe4m2PVGpySO-4XLtCpVXWEy0Mfoa_np8_ZPFu8vbzOpotMs4L3GbcVKUplSlEtJ4KZiuiCpXMVE5zlomJQcl3YQggwdmlKa1U-KVllQFnDKJ-M0O1-bxf89wZiLxsXNdS1aiH9QXIhOBeVSCDbgzr4GANY2QXXqPAjKZE7nXIt9zrlTudumnSm2PVh_2bZgDmGDi4TcHMAVNSqtkG12sUjxznP87JM3MOeg2Rj6yDIqB20GowLSaI03v1_yR_JXpcT</recordid><startdate>20050325</startdate><enddate>20050325</enddate><creator>Katiyar, Amit</creator><creator>Ji, Lei</creator><creator>Smirniotis, Panagiotis</creator><creator>Pinto, Neville G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050325</creationdate><title>Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size</title><author>Katiyar, Amit ; Ji, Lei ; Smirniotis, Panagiotis ; Pinto, Neville G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-6f9058ad879b374d90c54707a47642794e86c5f577edfbd8ffa23849deafd4163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adsorption</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kinetics</topic><topic>Mesoporous</topic><topic>Other chromatographic methods</topic><topic>Proteins</topic><topic>Proteins - chemistry</topic><topic>SBA-15</topic><topic>Silicates - chemistry</topic><topic>Size-selective</topic><topic>Static Electricity</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katiyar, Amit</creatorcontrib><creatorcontrib>Ji, Lei</creatorcontrib><creatorcontrib>Smirniotis, Panagiotis</creatorcontrib><creatorcontrib>Pinto, Neville G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katiyar, Amit</au><au>Ji, Lei</au><au>Smirniotis, Panagiotis</au><au>Pinto, Neville G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2005-03-25</date><risdate>2005</risdate><volume>1069</volume><issue>1</issue><spage>119</spage><epage>126</epage><pages>119-126</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>A mesoporous molecular sieve silicate, SBA-15, with three pore sizes (38.1
Å, 77.3
Å, and 240
Å) has been synthesized using a non-ionic, tri-block copolymer as a template in a sol–gel method. The effects of synthesis conditions on the pore size and pore-size distribution of this adsorbent have been described. The adsorption of proteins on these crystalline, ordered, materials has been studied. The kinetics of adsorption and equilibrium capacity have been probed with three proteins of different dimensions. The effects of electrostatic interactions and protein size are illustrated. It has been shown that SBA-15 materials can be tailored to show size selectivity for proteins, and very high capacities (450
mg/g) can be obtained. Furthermore, the rates of adsorption are shown to be dependent on the pore size, protein structure and solution pH.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15844490</pmid><doi>10.1016/j.chroma.2004.10.077</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of Chromatography A, 2005-03, Vol.1069 (1), p.119-126 |
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| subjects | Adsorption Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Fundamental and applied biological sciences. Psychology General aspects, investigation methods Hydrogen-Ion Concentration Kinetics Mesoporous Other chromatographic methods Proteins Proteins - chemistry SBA-15 Silicates - chemistry Size-selective Static Electricity X-Ray Diffraction |
| title | Protein adsorption on the mesoporous molecular sieve silicate SBA-15: effects of pH and pore size |
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