Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine
The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12 mM ammonium acetate and...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 2005-03, Vol.1068 (1), p.175-182 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 182 |
|---|---|
| container_issue | 1 |
| container_start_page | 175 |
| container_title | Journal of Chromatography A |
| container_volume | 1068 |
| creator | Rodríguez-Flores, J. Berzas Nevado, J.J. Contento Salcedo, A.M. Cabello Díaz, M.P. |
| description | The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12
mM ammonium acetate and 87.6
mM acetic acid in methanol–acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3
min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24
μg
L
−1 for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett–Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C
18 cartridge was necessary. Real determination of these analytes in two patient urines were done. |
| doi_str_mv | 10.1016/j.chroma.2004.09.089 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67765825</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967304017480</els_id><sourcerecordid>67765825</sourcerecordid><originalsourceid>FETCH-LOGICAL-c360t-e928ff3a5ca4fce9be519940f75dc03dbe7f144854a1aa63513e5d55ddfe39643</originalsourceid><addsrcrecordid>eNp9kD1PwzAQhj2AaCn8A4Q8sTXYiZ3ECxKq-JIqWGC2XPtMXCVxsZNK_fc4aiU2ptOdnrvT-yB0Q0lGCS3vt5lugu9UlhPCMiIyUoszNCckp0tRVsUMXca4JYRWpMov0IzymjHOyznq332vfkbwY8Ra7VzbqnDA0IIegt81PkB0EXcwNN5g6wMeGsCqV-1hmnuLv1uAPeg0M9gNCVWun3i18a0bAKeuGTvV4zG4Hq7QuVVthOtTXaCv56fP1ety_fHytnpcL3VRkmEJIq-tLRTXilkNYgOcCsGIrbjRpDAbqCxlrOZMUaXKgtMCuOHcGAuFKFmxQHfHu7vgU7o4yM5FDSldP0WVZVWVvM55AtkR1MHHGMDKXXBdciApkZNbuZVHt3JyK4mQyW1auz3dHzcdmL-lk9gEPBwBSCn3DoKM2kGvwbiQ3Erj3f8ffgFs8ZF-</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67765825</pqid></control><display><type>article</type><title>Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rodríguez-Flores, J. ; Berzas Nevado, J.J. ; Contento Salcedo, A.M. ; Cabello Díaz, M.P.</creator><creatorcontrib>Rodríguez-Flores, J. ; Berzas Nevado, J.J. ; Contento Salcedo, A.M. ; Cabello Díaz, M.P.</creatorcontrib><description>The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12
mM ammonium acetate and 87.6
mM acetic acid in methanol–acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3
min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24
μg
L
−1 for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett–Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C
18 cartridge was necessary. Real determination of these analytes in two patient urines were done.</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/j.chroma.2004.09.089</identifier><identifier>PMID: 15844556</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antineoplastic Agents - urine ; Benzamides ; Electrophoresis, Capillary - methods ; Gleevec ; Human urine ; Humans ; Imatinib Mesylate ; Nonaqueous capillary electrophoresis ; Piperazines - urine ; Pyrimidines - urine ; Ruggedness ; Sensitivity and Specificity ; Temperature</subject><ispartof>Journal of Chromatography A, 2005-03, Vol.1068 (1), p.175-182</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-e928ff3a5ca4fce9be519940f75dc03dbe7f144854a1aa63513e5d55ddfe39643</citedby><cites>FETCH-LOGICAL-c360t-e928ff3a5ca4fce9be519940f75dc03dbe7f144854a1aa63513e5d55ddfe39643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2004.09.089$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15844556$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodríguez-Flores, J.</creatorcontrib><creatorcontrib>Berzas Nevado, J.J.</creatorcontrib><creatorcontrib>Contento Salcedo, A.M.</creatorcontrib><creatorcontrib>Cabello Díaz, M.P.</creatorcontrib><title>Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12
mM ammonium acetate and 87.6
mM acetic acid in methanol–acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3
min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24
μg
L
−1 for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett–Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C
18 cartridge was necessary. Real determination of these analytes in two patient urines were done.</description><subject>Antineoplastic Agents - urine</subject><subject>Benzamides</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Gleevec</subject><subject>Human urine</subject><subject>Humans</subject><subject>Imatinib Mesylate</subject><subject>Nonaqueous capillary electrophoresis</subject><subject>Piperazines - urine</subject><subject>Pyrimidines - urine</subject><subject>Ruggedness</subject><subject>Sensitivity and Specificity</subject><subject>Temperature</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhj2AaCn8A4Q8sTXYiZ3ECxKq-JIqWGC2XPtMXCVxsZNK_fc4aiU2ptOdnrvT-yB0Q0lGCS3vt5lugu9UlhPCMiIyUoszNCckp0tRVsUMXca4JYRWpMov0IzymjHOyznq332vfkbwY8Ra7VzbqnDA0IIegt81PkB0EXcwNN5g6wMeGsCqV-1hmnuLv1uAPeg0M9gNCVWun3i18a0bAKeuGTvV4zG4Hq7QuVVthOtTXaCv56fP1ety_fHytnpcL3VRkmEJIq-tLRTXilkNYgOcCsGIrbjRpDAbqCxlrOZMUaXKgtMCuOHcGAuFKFmxQHfHu7vgU7o4yM5FDSldP0WVZVWVvM55AtkR1MHHGMDKXXBdciApkZNbuZVHt3JyK4mQyW1auz3dHzcdmL-lk9gEPBwBSCn3DoKM2kGvwbiQ3Erj3f8ffgFs8ZF-</recordid><startdate>20050311</startdate><enddate>20050311</enddate><creator>Rodríguez-Flores, J.</creator><creator>Berzas Nevado, J.J.</creator><creator>Contento Salcedo, A.M.</creator><creator>Cabello Díaz, M.P.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050311</creationdate><title>Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine</title><author>Rodríguez-Flores, J. ; Berzas Nevado, J.J. ; Contento Salcedo, A.M. ; Cabello Díaz, M.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-e928ff3a5ca4fce9be519940f75dc03dbe7f144854a1aa63513e5d55ddfe39643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Antineoplastic Agents - urine</topic><topic>Benzamides</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Gleevec</topic><topic>Human urine</topic><topic>Humans</topic><topic>Imatinib Mesylate</topic><topic>Nonaqueous capillary electrophoresis</topic><topic>Piperazines - urine</topic><topic>Pyrimidines - urine</topic><topic>Ruggedness</topic><topic>Sensitivity and Specificity</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez-Flores, J.</creatorcontrib><creatorcontrib>Berzas Nevado, J.J.</creatorcontrib><creatorcontrib>Contento Salcedo, A.M.</creatorcontrib><creatorcontrib>Cabello Díaz, M.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez-Flores, J.</au><au>Berzas Nevado, J.J.</au><au>Contento Salcedo, A.M.</au><au>Cabello Díaz, M.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2005-03-11</date><risdate>2005</risdate><volume>1068</volume><issue>1</issue><spage>175</spage><epage>182</epage><pages>175-182</pages><issn>0021-9673</issn><abstract>The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12
mM ammonium acetate and 87.6
mM acetic acid in methanol–acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3
min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24
μg
L
−1 for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett–Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C
18 cartridge was necessary. Real determination of these analytes in two patient urines were done.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15844556</pmid><doi>10.1016/j.chroma.2004.09.089</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 2005-03, Vol.1068 (1), p.175-182 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67765825 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Antineoplastic Agents - urine Benzamides Electrophoresis, Capillary - methods Gleevec Human urine Humans Imatinib Mesylate Nonaqueous capillary electrophoresis Piperazines - urine Pyrimidines - urine Ruggedness Sensitivity and Specificity Temperature |
| title | Nonaqueous capillary electrophoresis method for the analysis of gleevec and its main metabolite in human urine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T21%3A38%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Nonaqueous%20capillary%20electrophoresis%20method%20for%20the%20analysis%20of%20gleevec%20and%20its%20main%20metabolite%20in%20human%20urine&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Rodr%C3%ADguez-Flores,%20J.&rft.date=2005-03-11&rft.volume=1068&rft.issue=1&rft.spage=175&rft.epage=182&rft.pages=175-182&rft.issn=0021-9673&rft_id=info:doi/10.1016/j.chroma.2004.09.089&rft_dat=%3Cproquest_cross%3E67765825%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67765825&rft_id=info:pmid/15844556&rft_els_id=S0021967304017480&rfr_iscdi=true |