Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma
The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl-2, Bcl-XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis. Tissue samples were obtained from patients (age, 27-78 years) having surgery for pancr...
Gespeichert in:
| Veröffentlicht in: | Pancreas 2005-05, Vol.30 (4), p.337-342 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 342 |
|---|---|
| container_issue | 4 |
| container_start_page | 337 |
| container_title | Pancreas |
| container_volume | 30 |
| creator | Sharma, Jyotika Srinivasan, Radhika Majumdar, Siddhartha Mir, Snober Radotra, Bishan Dass Wig, Jai Dev |
| description | The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl-2, Bcl-XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis.
Tissue samples were obtained from patients (age, 27-78 years) having surgery for pancreatic cancer. Normal pancreatic tissue away from the main tumor mass was also analyzed. The levels of Bcl-2, Bcl-XL, and Bax mRNA expression were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The presence of corresponding proteins was determined by immunohistochemistry (IHC). The apoptotic index was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay.
A total of 25 cases were analyzed. The apoptotic index (percentage) ranged from 0.0% to 1.8%, with a median of 0.26. Semiquantitative RT-PCR revealed variable mRNA expression, with the Bcl-2/Bax ratio ranging from 0.2 to 1.5 and the Bcl-XL/Bax ratio ranging from 0.3 to 1.8. There was no correlation of mRNA levels with the apoptotic index. Immunohistochemical analysis showed positive Bcl-2, Bax, Bcl-XL expression in 20%, 72%, and 92% of cancer samples; however, their levels were variable. Spearman rank correlation coefficient test revealed a significant inverse association for the Bcl-XL IHC score and apoptotic index (P < 0.05). In contrast, Bcl-2, Bax protein levels did not show any association with the apoptotic index. However, as compared with the normal pancreas, Bcl-2, Bcl-XL, Bax were overexpressed in most of the pancreatic cancer samples (Mann-Whitney U test, P < 0.01).
In pancreatic cancer, there is an upregulation of all the apoptotic regulatory molecules and the apoptotic index is chiefly determined by Bcl-XL protein levels. |
| doi_str_mv | 10.1097/01.mpa.0000160282.64451.f1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67761868</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67761868</sourcerecordid><originalsourceid>FETCH-LOGICAL-c232t-b3d02d853758830f6bca76310672c2ab85392ade32d7d3e163e1e867ea141b753</originalsourceid><addsrcrecordid>eNpFkEtLAzEQx4Motla_giwevO2aSTaPetOiVSh4UfAWssksrOzLZCv67U1twYGZgZn_PPgRcgW0ALpUNxSKbrQFTQaSMs0KWZYCihqOyBwEl3mpmT4mc6q1yDkoNSNnMX4kueJieUpmIHQJtCznZH3v2vx9k41hmLDpsxa_sI2ZxwlD1_SY2XEYp2FqXNb0Hr9TzEbbu4B2V3M2uKYfOntOTmrbRrw45AV5e3x4XT3lm5f18-pukzvG2ZRX3FPmteBKaM1pLStnleRApWKO2Sp1lsx65MwrzxFkctRSoYUSKiX4glzv96aHP7cYJ9M10WHb2h6HbTRSKQla6iS83QtdGGIMWJsxNJ0NPwao2WE0FEzCaP4xmj-MpoY0fHm4sq069P-jB278F_aubuA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67761868</pqid></control><display><type>article</type><title>Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma</title><source>MEDLINE</source><source>Journals@Ovid Ovid Autoload</source><creator>Sharma, Jyotika ; Srinivasan, Radhika ; Majumdar, Siddhartha ; Mir, Snober ; Radotra, Bishan Dass ; Wig, Jai Dev</creator><creatorcontrib>Sharma, Jyotika ; Srinivasan, Radhika ; Majumdar, Siddhartha ; Mir, Snober ; Radotra, Bishan Dass ; Wig, Jai Dev</creatorcontrib><description>The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl-2, Bcl-XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis.
Tissue samples were obtained from patients (age, 27-78 years) having surgery for pancreatic cancer. Normal pancreatic tissue away from the main tumor mass was also analyzed. The levels of Bcl-2, Bcl-XL, and Bax mRNA expression were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The presence of corresponding proteins was determined by immunohistochemistry (IHC). The apoptotic index was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay.
A total of 25 cases were analyzed. The apoptotic index (percentage) ranged from 0.0% to 1.8%, with a median of 0.26. Semiquantitative RT-PCR revealed variable mRNA expression, with the Bcl-2/Bax ratio ranging from 0.2 to 1.5 and the Bcl-XL/Bax ratio ranging from 0.3 to 1.8. There was no correlation of mRNA levels with the apoptotic index. Immunohistochemical analysis showed positive Bcl-2, Bax, Bcl-XL expression in 20%, 72%, and 92% of cancer samples; however, their levels were variable. Spearman rank correlation coefficient test revealed a significant inverse association for the Bcl-XL IHC score and apoptotic index (P < 0.05). In contrast, Bcl-2, Bax protein levels did not show any association with the apoptotic index. However, as compared with the normal pancreas, Bcl-2, Bcl-XL, Bax were overexpressed in most of the pancreatic cancer samples (Mann-Whitney U test, P < 0.01).
In pancreatic cancer, there is an upregulation of all the apoptotic regulatory molecules and the apoptotic index is chiefly determined by Bcl-XL protein levels.</description><identifier>ISSN: 0885-3177</identifier><identifier>EISSN: 1536-4828</identifier><identifier>DOI: 10.1097/01.mpa.0000160282.64451.f1</identifier><identifier>PMID: 15841044</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; Apoptosis ; bcl-2-Associated X Protein - genetics ; bcl-2-Associated X Protein - metabolism ; bcl-X Protein - genetics ; bcl-X Protein - metabolism ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Carcinoma, Pancreatic Ductal - metabolism ; Carcinoma, Pancreatic Ductal - pathology ; Female ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Male ; Middle Aged ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Up-Regulation</subject><ispartof>Pancreas, 2005-05, Vol.30 (4), p.337-342</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c232t-b3d02d853758830f6bca76310672c2ab85392ade32d7d3e163e1e867ea141b753</citedby><cites>FETCH-LOGICAL-c232t-b3d02d853758830f6bca76310672c2ab85392ade32d7d3e163e1e867ea141b753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15841044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Jyotika</creatorcontrib><creatorcontrib>Srinivasan, Radhika</creatorcontrib><creatorcontrib>Majumdar, Siddhartha</creatorcontrib><creatorcontrib>Mir, Snober</creatorcontrib><creatorcontrib>Radotra, Bishan Dass</creatorcontrib><creatorcontrib>Wig, Jai Dev</creatorcontrib><title>Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma</title><title>Pancreas</title><addtitle>Pancreas</addtitle><description>The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl-2, Bcl-XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis.
Tissue samples were obtained from patients (age, 27-78 years) having surgery for pancreatic cancer. Normal pancreatic tissue away from the main tumor mass was also analyzed. The levels of Bcl-2, Bcl-XL, and Bax mRNA expression were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The presence of corresponding proteins was determined by immunohistochemistry (IHC). The apoptotic index was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay.
A total of 25 cases were analyzed. The apoptotic index (percentage) ranged from 0.0% to 1.8%, with a median of 0.26. Semiquantitative RT-PCR revealed variable mRNA expression, with the Bcl-2/Bax ratio ranging from 0.2 to 1.5 and the Bcl-XL/Bax ratio ranging from 0.3 to 1.8. There was no correlation of mRNA levels with the apoptotic index. Immunohistochemical analysis showed positive Bcl-2, Bax, Bcl-XL expression in 20%, 72%, and 92% of cancer samples; however, their levels were variable. Spearman rank correlation coefficient test revealed a significant inverse association for the Bcl-XL IHC score and apoptotic index (P < 0.05). In contrast, Bcl-2, Bax protein levels did not show any association with the apoptotic index. However, as compared with the normal pancreas, Bcl-2, Bcl-XL, Bax were overexpressed in most of the pancreatic cancer samples (Mann-Whitney U test, P < 0.01).
In pancreatic cancer, there is an upregulation of all the apoptotic regulatory molecules and the apoptotic index is chiefly determined by Bcl-XL protein levels.</description><subject>Adult</subject><subject>Aged</subject><subject>Apoptosis</subject><subject>bcl-2-Associated X Protein - genetics</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>bcl-X Protein - genetics</subject><subject>bcl-X Protein - metabolism</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - metabolism</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Up-Regulation</subject><issn>0885-3177</issn><issn>1536-4828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEQx4Motla_giwevO2aSTaPetOiVSh4UfAWssksrOzLZCv67U1twYGZgZn_PPgRcgW0ALpUNxSKbrQFTQaSMs0KWZYCihqOyBwEl3mpmT4mc6q1yDkoNSNnMX4kueJieUpmIHQJtCznZH3v2vx9k41hmLDpsxa_sI2ZxwlD1_SY2XEYp2FqXNb0Hr9TzEbbu4B2V3M2uKYfOntOTmrbRrw45AV5e3x4XT3lm5f18-pukzvG2ZRX3FPmteBKaM1pLStnleRApWKO2Sp1lsx65MwrzxFkctRSoYUSKiX4glzv96aHP7cYJ9M10WHb2h6HbTRSKQla6iS83QtdGGIMWJsxNJ0NPwao2WE0FEzCaP4xmj-MpoY0fHm4sq069P-jB278F_aubuA</recordid><startdate>200505</startdate><enddate>200505</enddate><creator>Sharma, Jyotika</creator><creator>Srinivasan, Radhika</creator><creator>Majumdar, Siddhartha</creator><creator>Mir, Snober</creator><creator>Radotra, Bishan Dass</creator><creator>Wig, Jai Dev</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200505</creationdate><title>Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma</title><author>Sharma, Jyotika ; Srinivasan, Radhika ; Majumdar, Siddhartha ; Mir, Snober ; Radotra, Bishan Dass ; Wig, Jai Dev</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c232t-b3d02d853758830f6bca76310672c2ab85392ade32d7d3e163e1e867ea141b753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Apoptosis</topic><topic>bcl-2-Associated X Protein - genetics</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>bcl-X Protein - genetics</topic><topic>bcl-X Protein - metabolism</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - metabolism</topic><topic>Carcinoma, Pancreatic Ductal - pathology</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - genetics</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Jyotika</creatorcontrib><creatorcontrib>Srinivasan, Radhika</creatorcontrib><creatorcontrib>Majumdar, Siddhartha</creatorcontrib><creatorcontrib>Mir, Snober</creatorcontrib><creatorcontrib>Radotra, Bishan Dass</creatorcontrib><creatorcontrib>Wig, Jai Dev</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pancreas</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Jyotika</au><au>Srinivasan, Radhika</au><au>Majumdar, Siddhartha</au><au>Mir, Snober</au><au>Radotra, Bishan Dass</au><au>Wig, Jai Dev</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma</atitle><jtitle>Pancreas</jtitle><addtitle>Pancreas</addtitle><date>2005-05</date><risdate>2005</risdate><volume>30</volume><issue>4</issue><spage>337</spage><epage>342</epage><pages>337-342</pages><issn>0885-3177</issn><eissn>1536-4828</eissn><abstract>The present study was designed to analyze the expression of the major antiapoptotic molecules Bcl-2, Bcl-XL and the proapoptotic Bax in pancreatic ductal carcinoma and their correlation to the extent of apoptosis.
Tissue samples were obtained from patients (age, 27-78 years) having surgery for pancreatic cancer. Normal pancreatic tissue away from the main tumor mass was also analyzed. The levels of Bcl-2, Bcl-XL, and Bax mRNA expression were analyzed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR). The presence of corresponding proteins was determined by immunohistochemistry (IHC). The apoptotic index was determined by terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) assay.
A total of 25 cases were analyzed. The apoptotic index (percentage) ranged from 0.0% to 1.8%, with a median of 0.26. Semiquantitative RT-PCR revealed variable mRNA expression, with the Bcl-2/Bax ratio ranging from 0.2 to 1.5 and the Bcl-XL/Bax ratio ranging from 0.3 to 1.8. There was no correlation of mRNA levels with the apoptotic index. Immunohistochemical analysis showed positive Bcl-2, Bax, Bcl-XL expression in 20%, 72%, and 92% of cancer samples; however, their levels were variable. Spearman rank correlation coefficient test revealed a significant inverse association for the Bcl-XL IHC score and apoptotic index (P < 0.05). In contrast, Bcl-2, Bax protein levels did not show any association with the apoptotic index. However, as compared with the normal pancreas, Bcl-2, Bcl-XL, Bax were overexpressed in most of the pancreatic cancer samples (Mann-Whitney U test, P < 0.01).
In pancreatic cancer, there is an upregulation of all the apoptotic regulatory molecules and the apoptotic index is chiefly determined by Bcl-XL protein levels.</abstract><cop>United States</cop><pmid>15841044</pmid><doi>10.1097/01.mpa.0000160282.64451.f1</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-3177 |
| ispartof | Pancreas, 2005-05, Vol.30 (4), p.337-342 |
| issn | 0885-3177 1536-4828 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67761868 |
| source | MEDLINE; Journals@Ovid Ovid Autoload |
| subjects | Adult Aged Apoptosis bcl-2-Associated X Protein - genetics bcl-2-Associated X Protein - metabolism bcl-X Protein - genetics bcl-X Protein - metabolism Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Carcinoma, Pancreatic Ductal - metabolism Carcinoma, Pancreatic Ductal - pathology Female Humans Immunohistochemistry In Situ Nick-End Labeling Male Middle Aged Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Up-Regulation |
| title | Bcl-XL protein levels determine apoptotic index in pancreatic carcinoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T04%3A24%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Bcl-XL%20protein%20levels%20determine%20apoptotic%20index%20in%20pancreatic%20carcinoma&rft.jtitle=Pancreas&rft.au=Sharma,%20Jyotika&rft.date=2005-05&rft.volume=30&rft.issue=4&rft.spage=337&rft.epage=342&rft.pages=337-342&rft.issn=0885-3177&rft.eissn=1536-4828&rft_id=info:doi/10.1097/01.mpa.0000160282.64451.f1&rft_dat=%3Cproquest_cross%3E67761868%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67761868&rft_id=info:pmid/15841044&rfr_iscdi=true |