Continuous Assays for DNA Translocation Using Fluorescent Triplex Dissociation: Application to Type I Restriction Endonucleases

Fluorescent assays and accompanying kinetic models are described for the analysis of DNA translocation independent of duplex unwinding. A triplex binding site (TBS) was introduced into DNA substrates at precise loci downstream of recognition sequences for type IA, IB and IC restriction endonucleases...

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Veröffentlicht in:	Journal of molecular biology 2005-05, Vol.348 (4), p.895-915
Hauptverfasser:	McClelland, Sarah E., Dryden, David T.F., Szczelkun, Mark D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Deoxyribonucleases, Type I Site-Specific - metabolism DNA - chemistry DNA - genetics DNA - metabolism DNA helicase DNA translocation fast reaction kinetics Fluorescence Polarization Genes, Reporter - genetics Kinetics motor protein Movement Mutation - genetics Spectrometry, Fluorescence Substrate Specificity triplex
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