Effect of Cryopreservation on Sea Bass Sperm Proteins
In the present study we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to verify whether the protein expression of sea bass sperm was affected by the cryopreservation procedure. The protein...
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| Veröffentlicht in: | Biology of reproduction 2005-05, Vol.72 (5), p.1262-1267 |
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| creator | Zilli, Loredana Schiavone, Roberta Zonno, Vincenzo Rossano, Rocco Storelli, Carlo Vilella, Sebastiano |
| description | In the present study we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to verify whether the protein expression of sea bass sperm was affected by the cryopreservation procedure. The protein profiles differed between fresh and frozen-thawed semen as revealed by visual inspection and by image analysis software. We identified 163 spots in fresh sperm; among these, 13 were significantly decreased and 8 were absent in two-dimensional gel obtained with cryopreserved sperm. Five of these spots were analyzed with MALDI-TOF, but only three showed a significant match in the databases used in bio-informatics analysis (PeptIdent, Mascot, and MS-Fit). In particular, spot 5 showed homology with a novel protein of zebrafish (similar to SKB1 of human and mouse), spot 13 showed homology with amphibian G1/S-specific cyclin E2, and spot 20 showed homology with the hypothetical protein DKFZp566A1524 of Brachidanio rerio. The present work shows that the use of the cryopreservation procedure causes the degradation of sperm proteins and among these, two could be at least partially responsible for the observed decrease in sperm motility duration and the lower hatching rate of eggs fertilized with cryopreserved sperm. |
| doi_str_mv | 10.1095/biolreprod.104.036202 |
| format | Article |
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The protein profiles differed between fresh and frozen-thawed semen as revealed by visual inspection and by image analysis software. We identified 163 spots in fresh sperm; among these, 13 were significantly decreased and 8 were absent in two-dimensional gel obtained with cryopreserved sperm. Five of these spots were analyzed with MALDI-TOF, but only three showed a significant match in the databases used in bio-informatics analysis (PeptIdent, Mascot, and MS-Fit). In particular, spot 5 showed homology with a novel protein of zebrafish (similar to SKB1 of human and mouse), spot 13 showed homology with amphibian G1/S-specific cyclin E2, and spot 20 showed homology with the hypothetical protein DKFZp566A1524 of Brachidanio rerio. The present work shows that the use of the cryopreservation procedure causes the degradation of sperm proteins and among these, two could be at least partially responsible for the observed decrease in sperm motility duration and the lower hatching rate of eggs fertilized with cryopreserved sperm.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.104.036202</identifier><identifier>PMID: 15659707</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Bass - metabolism ; Biological and medical sciences ; Contents ; Cryopreservation ; Danio rerio ; Dicentrarchus labrax ; Electrophoresis, Gel, Two-Dimensional ; fertilization ; Fish Proteins - isolation & purification ; Fish Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; gamete biology ; Male ; Mammalian male genital system ; Morphology. Physiology ; Peptide Mapping ; Semen Preservation ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; sperm ; Spermatozoa - metabolism ; Vertebrates: reproduction ; Xenopus Proteins - isolation & purification ; Zebrafish Proteins - isolation & purification</subject><ispartof>Biology of reproduction, 2005-05, Vol.72 (5), p.1262-1267</ispartof><rights>Society for the Study of Reproduction</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b420t-9a5c11a7d91f4b0f3dd915e6faf299f9f4c97d75fc87d117082fa2d34bddef813</citedby><cites>FETCH-LOGICAL-b420t-9a5c11a7d91f4b0f3dd915e6faf299f9f4c97d75fc87d117082fa2d34bddef813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://bioone.org/doi/pdf/10.1095/biolreprod.104.036202$$EPDF$$P50$$Gbioone$$H</linktopdf><link.rule.ids>314,777,781,26959,27905,27906,52344</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16714208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15659707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zilli, Loredana</creatorcontrib><creatorcontrib>Schiavone, Roberta</creatorcontrib><creatorcontrib>Zonno, Vincenzo</creatorcontrib><creatorcontrib>Rossano, Rocco</creatorcontrib><creatorcontrib>Storelli, Carlo</creatorcontrib><creatorcontrib>Vilella, Sebastiano</creatorcontrib><title>Effect of Cryopreservation on Sea Bass Sperm Proteins</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>In the present study we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to verify whether the protein expression of sea bass sperm was affected by the cryopreservation procedure. The protein profiles differed between fresh and frozen-thawed semen as revealed by visual inspection and by image analysis software. We identified 163 spots in fresh sperm; among these, 13 were significantly decreased and 8 were absent in two-dimensional gel obtained with cryopreserved sperm. Five of these spots were analyzed with MALDI-TOF, but only three showed a significant match in the databases used in bio-informatics analysis (PeptIdent, Mascot, and MS-Fit). In particular, spot 5 showed homology with a novel protein of zebrafish (similar to SKB1 of human and mouse), spot 13 showed homology with amphibian G1/S-specific cyclin E2, and spot 20 showed homology with the hypothetical protein DKFZp566A1524 of Brachidanio rerio. The present work shows that the use of the cryopreservation procedure causes the degradation of sperm proteins and among these, two could be at least partially responsible for the observed decrease in sperm motility duration and the lower hatching rate of eggs fertilized with cryopreserved sperm.</description><subject>Animals</subject><subject>Bass - metabolism</subject><subject>Biological and medical sciences</subject><subject>Contents</subject><subject>Cryopreservation</subject><subject>Danio rerio</subject><subject>Dicentrarchus labrax</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>fertilization</subject><subject>Fish Proteins - isolation & purification</subject><subject>Fish Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamete biology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Peptide Mapping</subject><subject>Semen Preservation</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>sperm</subject><subject>Spermatozoa - metabolism</subject><subject>Vertebrates: reproduction</subject><subject>Xenopus Proteins - isolation & purification</subject><subject>Zebrafish Proteins - isolation & purification</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1LwzAUhoMoOj9-gtIbvavmo0maSx3zAwSF6XVImxOttM1MOsv-vRkb7FIhkBx4zjlvHoTOCb4mWPGbqvFtgEXwNtXFNWaCYrqHJoRTlUsqyn00wRiLnDHBjtBxjF8Yk4JRdoiOCBdcSSwniM-cg3rIvMumYeUXASKEHzM0vs_SmYPJ7kyM2XwBoctegx-g6eMpOnCmjXC2vU_Q-_3sbfqYP788PE1vn_OqoHjIleE1IUZaRVxRYcdsenEQzjiqlFOuqJW0kru6lJYQiUvqDLWsqKwFVxJ2gq42c9M_v5cQB901sYa2NT34ZdRCSkE4F3-CyU2BeSkTyDdgHXyMAZxehKYzYaUJ1muxeic21YXeiE19F9sFy6oDu-vamkzA5RYwsTatC6avm7jjhCTJSblL-tl8fI5NAB0707ZpLNPjOEqquSZUrDeyDZgC-R7-mfMXVC-hmA</recordid><startdate>20050501</startdate><enddate>20050501</enddate><creator>Zilli, Loredana</creator><creator>Schiavone, Roberta</creator><creator>Zonno, Vincenzo</creator><creator>Rossano, Rocco</creator><creator>Storelli, Carlo</creator><creator>Vilella, Sebastiano</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050501</creationdate><title>Effect of Cryopreservation on Sea Bass Sperm Proteins</title><author>Zilli, Loredana ; Schiavone, Roberta ; Zonno, Vincenzo ; Rossano, Rocco ; Storelli, Carlo ; Vilella, Sebastiano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b420t-9a5c11a7d91f4b0f3dd915e6faf299f9f4c97d75fc87d117082fa2d34bddef813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Bass - metabolism</topic><topic>Biological and medical sciences</topic><topic>Contents</topic><topic>Cryopreservation</topic><topic>Danio rerio</topic><topic>Dicentrarchus labrax</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>fertilization</topic><topic>Fish Proteins - isolation & purification</topic><topic>Fish Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamete biology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Peptide Mapping</topic><topic>Semen Preservation</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>sperm</topic><topic>Spermatozoa - metabolism</topic><topic>Vertebrates: reproduction</topic><topic>Xenopus Proteins - isolation & purification</topic><topic>Zebrafish Proteins - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zilli, Loredana</creatorcontrib><creatorcontrib>Schiavone, Roberta</creatorcontrib><creatorcontrib>Zonno, Vincenzo</creatorcontrib><creatorcontrib>Rossano, Rocco</creatorcontrib><creatorcontrib>Storelli, Carlo</creatorcontrib><creatorcontrib>Vilella, Sebastiano</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zilli, Loredana</au><au>Schiavone, Roberta</au><au>Zonno, Vincenzo</au><au>Rossano, Rocco</au><au>Storelli, Carlo</au><au>Vilella, Sebastiano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Cryopreservation on Sea Bass Sperm Proteins</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2005-05-01</date><risdate>2005</risdate><volume>72</volume><issue>5</issue><spage>1262</spage><epage>1267</epage><pages>1262-1267</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>In the present study we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to verify whether the protein expression of sea bass sperm was affected by the cryopreservation procedure. The protein profiles differed between fresh and frozen-thawed semen as revealed by visual inspection and by image analysis software. We identified 163 spots in fresh sperm; among these, 13 were significantly decreased and 8 were absent in two-dimensional gel obtained with cryopreserved sperm. Five of these spots were analyzed with MALDI-TOF, but only three showed a significant match in the databases used in bio-informatics analysis (PeptIdent, Mascot, and MS-Fit). In particular, spot 5 showed homology with a novel protein of zebrafish (similar to SKB1 of human and mouse), spot 13 showed homology with amphibian G1/S-specific cyclin E2, and spot 20 showed homology with the hypothetical protein DKFZp566A1524 of Brachidanio rerio. The present work shows that the use of the cryopreservation procedure causes the degradation of sperm proteins and among these, two could be at least partially responsible for the observed decrease in sperm motility duration and the lower hatching rate of eggs fertilized with cryopreserved sperm.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15659707</pmid><doi>10.1095/biolreprod.104.036202</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB Electronic Journals Library |
| subjects | Animals Bass - metabolism Biological and medical sciences Contents Cryopreservation Danio rerio Dicentrarchus labrax Electrophoresis, Gel, Two-Dimensional fertilization Fish Proteins - isolation & purification Fish Proteins - metabolism Fundamental and applied biological sciences. Psychology gamete biology Male Mammalian male genital system Morphology. Physiology Peptide Mapping Semen Preservation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization sperm Spermatozoa - metabolism Vertebrates: reproduction Xenopus Proteins - isolation & purification Zebrafish Proteins - isolation & purification |
| title | Effect of Cryopreservation on Sea Bass Sperm Proteins |
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