Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline
Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50 μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was perfor...
Gespeichert in:
| Veröffentlicht in: | Bioorganic & medicinal chemistry letters 2006-05, Vol.16 (9), p.2407-2410 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2410 |
|---|---|
| container_issue | 9 |
| container_start_page | 2407 |
| container_title | Bioorganic & medicinal chemistry letters |
| container_volume | 16 |
| creator | Adamczyk, Maciej Brashear, R. Jeffrey Mattingly, Phillip G. |
| description | Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50
μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1
μL) and analysis time ( |
| doi_str_mv | 10.1016/j.bmcl.2006.01.114 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67759883</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960894X06001557</els_id><sourcerecordid>67759883</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-a9b3698b99dc0ce97d09f0fb8a1e96e618ceaa3d693ee47cb8ffc29306c9443b3</originalsourceid><addsrcrecordid>eNqFkU2LFDEQhoMo7rj6BzxILnrrNplk0h3xsix-wYIgCt5CdVI9naHTaZNu3f0B_m8zzsDe9FSHet7ipR5CnnNWc8bV60PdBTvWW8ZUzXjNuXxANlwqWQnJdg_JhmnFqlbL7xfkSc4HxrhkUj4mF1zJtmmY3JDfX2D2jg5-P1TLkOK6H-Z1oQ4XtIuPE409nTHFW--Q_vLLQGGiYJN3fvJrqHRlIXXxFkIB3tArOsQQ9zhhXDO1AwY_rsFPmC1OC4Wc4Y72MdF5hBygEHEs26fkUQ9jxmfneUm-vX_39fpjdfP5w6frq5vKSr5bKtCdULrttHaWWdSNY7pnfdcCR61Q8dYigHBKC0TZ2K7te7vVgimrpRSduCSvTnfnFH-smBcTfGk2jvC3sFFNs9NtK_4Lci0aJfm2gNsTaFPMOWFv5uQDpDvDmTlaMgdztGSOlgzjplgqoRfn62sX0N1HzloK8PIMQLYw9gkm6_M91yiuudgV7u2Jw_K0nx6TydbjZNH5VPwZF_2_evwBf8ezlg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19376412</pqid></control><display><type>article</type><title>Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Adamczyk, Maciej ; Brashear, R. Jeffrey ; Mattingly, Phillip G.</creator><creatorcontrib>Adamczyk, Maciej ; Brashear, R. Jeffrey ; Mattingly, Phillip G.</creatorcontrib><description>Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50
μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1
μL) and analysis time (<5
s per well).</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/j.bmcl.2006.01.114</identifier><identifier>PMID: 16487704</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Acridines - chemistry ; Acridinium-9-carboxamide ; Analysis ; Betaine - chemical synthesis ; Biological and medical sciences ; Chemiluminescence ; Choline ; Choline - blood ; Choline - chemistry ; General pharmacology ; Homogeneous assay ; Humans ; Hydrogen Peroxide - analysis ; Investigative techniques, diagnostic techniques (general aspects) ; Luminescent Measurements - methods ; Medical sciences ; Miscellaneous. Technology ; Molecular Structure ; Oxidation-Reduction ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pharmacology. Drug treatments ; Sensitivity and Specificity ; Time Factors</subject><ispartof>Bioorganic & medicinal chemistry letters, 2006-05, Vol.16 (9), p.2407-2410</ispartof><rights>2006 Elsevier Ltd</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-a9b3698b99dc0ce97d09f0fb8a1e96e618ceaa3d693ee47cb8ffc29306c9443b3</citedby><cites>FETCH-LOGICAL-c415t-a9b3698b99dc0ce97d09f0fb8a1e96e618ceaa3d693ee47cb8ffc29306c9443b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bmcl.2006.01.114$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17619135$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16487704$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adamczyk, Maciej</creatorcontrib><creatorcontrib>Brashear, R. Jeffrey</creatorcontrib><creatorcontrib>Mattingly, Phillip G.</creatorcontrib><title>Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50
μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1
μL) and analysis time (<5
s per well).</description><subject>Acridines - chemistry</subject><subject>Acridinium-9-carboxamide</subject><subject>Analysis</subject><subject>Betaine - chemical synthesis</subject><subject>Biological and medical sciences</subject><subject>Chemiluminescence</subject><subject>Choline</subject><subject>Choline - blood</subject><subject>Choline - chemistry</subject><subject>General pharmacology</subject><subject>Homogeneous assay</subject><subject>Humans</subject><subject>Hydrogen Peroxide - analysis</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Luminescent Measurements - methods</subject><subject>Medical sciences</subject><subject>Miscellaneous. Technology</subject><subject>Molecular Structure</subject><subject>Oxidation-Reduction</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pharmacology. Drug treatments</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEQhoMo7rj6BzxILnrrNplk0h3xsix-wYIgCt5CdVI9naHTaZNu3f0B_m8zzsDe9FSHet7ipR5CnnNWc8bV60PdBTvWW8ZUzXjNuXxANlwqWQnJdg_JhmnFqlbL7xfkSc4HxrhkUj4mF1zJtmmY3JDfX2D2jg5-P1TLkOK6H-Z1oQ4XtIuPE409nTHFW--Q_vLLQGGiYJN3fvJrqHRlIXXxFkIB3tArOsQQ9zhhXDO1AwY_rsFPmC1OC4Wc4Y72MdF5hBygEHEs26fkUQ9jxmfneUm-vX_39fpjdfP5w6frq5vKSr5bKtCdULrttHaWWdSNY7pnfdcCR61Q8dYigHBKC0TZ2K7te7vVgimrpRSduCSvTnfnFH-smBcTfGk2jvC3sFFNs9NtK_4Lci0aJfm2gNsTaFPMOWFv5uQDpDvDmTlaMgdztGSOlgzjplgqoRfn62sX0N1HzloK8PIMQLYw9gkm6_M91yiuudgV7u2Jw_K0nx6TydbjZNH5VPwZF_2_evwBf8ezlg</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Adamczyk, Maciej</creator><creator>Brashear, R. Jeffrey</creator><creator>Mattingly, Phillip G.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline</title><author>Adamczyk, Maciej ; Brashear, R. Jeffrey ; Mattingly, Phillip G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-a9b3698b99dc0ce97d09f0fb8a1e96e618ceaa3d693ee47cb8ffc29306c9443b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Acridines - chemistry</topic><topic>Acridinium-9-carboxamide</topic><topic>Analysis</topic><topic>Betaine - chemical synthesis</topic><topic>Biological and medical sciences</topic><topic>Chemiluminescence</topic><topic>Choline</topic><topic>Choline - blood</topic><topic>Choline - chemistry</topic><topic>General pharmacology</topic><topic>Homogeneous assay</topic><topic>Humans</topic><topic>Hydrogen Peroxide - analysis</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Luminescent Measurements - methods</topic><topic>Medical sciences</topic><topic>Miscellaneous. Technology</topic><topic>Molecular Structure</topic><topic>Oxidation-Reduction</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pharmacology. Drug treatments</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adamczyk, Maciej</creatorcontrib><creatorcontrib>Brashear, R. Jeffrey</creatorcontrib><creatorcontrib>Mattingly, Phillip G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adamczyk, Maciej</au><au>Brashear, R. Jeffrey</au><au>Mattingly, Phillip G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>16</volume><issue>9</issue><spage>2407</spage><epage>2410</epage><pages>2407-2410</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>Hydrogen peroxide generated from the enzymatic oxidation of choline was detected using a chemiluminescent acridinium-9-carboxamide. The dose–response for choline (0–50
μM) was established in buffer and was applicable to the quantification of choline in human plasma. This homogeneous assay was performed in a 96-well microplate format and required minimal sample volume (1
μL) and analysis time (<5
s per well).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>16487704</pmid><doi>10.1016/j.bmcl.2006.01.114</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-894X |
| ispartof | Bioorganic & medicinal chemistry letters, 2006-05, Vol.16 (9), p.2407-2410 |
| issn | 0960-894X 1464-3405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67759883 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acridines - chemistry Acridinium-9-carboxamide Analysis Betaine - chemical synthesis Biological and medical sciences Chemiluminescence Choline Choline - blood Choline - chemistry General pharmacology Homogeneous assay Humans Hydrogen Peroxide - analysis Investigative techniques, diagnostic techniques (general aspects) Luminescent Measurements - methods Medical sciences Miscellaneous. Technology Molecular Structure Oxidation-Reduction Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pharmacology. Drug treatments Sensitivity and Specificity Time Factors |
| title | Rapid high-throughput detection of peroxide with an acridinium-9-carboxamide: A homogeneous chemiluminescent assay for plasma choline |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-11-29T09%3A37%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20high-throughput%20detection%20of%20peroxide%20with%20an%20acridinium-9-carboxamide:%20A%20homogeneous%20chemiluminescent%20assay%20for%20plasma%20choline&rft.jtitle=Bioorganic%20&%20medicinal%20chemistry%20letters&rft.au=Adamczyk,%20Maciej&rft.date=2006-05-01&rft.volume=16&rft.issue=9&rft.spage=2407&rft.epage=2410&rft.pages=2407-2410&rft.issn=0960-894X&rft.eissn=1464-3405&rft_id=info:doi/10.1016/j.bmcl.2006.01.114&rft_dat=%3Cproquest_cross%3E67759883%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19376412&rft_id=info:pmid/16487704&rft_els_id=S0960894X06001557&rfr_iscdi=true |