Application of the ferrous oxidation–xylenol orange assay for the screening of 5-lipoxygenase inhibitors

5-Lipoxygenase (5-LO) is the key enzyme involved in leukotriene synthesis and its improper regulation is implicated in several inflammatory diseases. A rapid and sensitive assay for 5-LO activity suitable for high-throughput format is not yet available. In this study, we examined whether the ferrous...

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Veröffentlicht in:	Analytical biochemistry 2006-04, Vol.351 (1), p.62-68
Hauptverfasser:	Cho, Young Sik, Kim, Hyo Sun, Kim, Chi Hyun, Cheon, Hyae Gyeong
Format:	Artikel
Sprache:	eng
Schlagworte:	5-Lipoxygenase Animals Base Sequence Cell Line DNA Primers Electrophoresis, Polyacrylamide Gel Ferrous Compounds - chemistry FOX assay High-throughput screening Lipoxygenase Inhibitors - analysis Oxidation-Reduction Phenols Rats Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet Spodoptera Sulfoxides Xylenes - chemistry Zileuton
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