Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin
Free radicals/reactive oxygen species (ROS) generated in skin by UV irradiation were measured by electron spin resonance (ESR). To increase the sensitivity of measurement the short life free radicals/ROS were scavenged and accumulated by using the nitroxyl probe 3-carboxy-2,2,5,5-tetrametylpyrrolidi...
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| Veröffentlicht in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2006-03, Vol.63 (4), p.840-845 |
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| creator | Herrling, Th Jung, K. Fuchs, J. |
| description | Free radicals/reactive oxygen species (ROS) generated in skin by UV irradiation were measured by electron spin resonance (ESR). To increase the sensitivity of measurement the short life free radicals/ROS were scavenged and accumulated by using the nitroxyl probe 3-carboxy-2,2,5,5-tetrametylpyrrolidine-1-oxyl (PCA). The spatial distribution of free radicals/ROS measured in pig skin biopsies with ESR imaging after UV irradiation corresponds to the intensity decay of irradiance in the depth of the skin. The main part of free radicals/ROS were generated by UVA (320–400
nm) so that the spatial distribution of free radicals reaches up to the lower side of the dermis. In vivo measurements on human skin were performed with a L-band ESR spectrometer and a surface coil integrating the signal intensities from all skin layers to get a sufficient signal amplitude. Using this experimental arrangement the protection of UVB and UVA/B filter against the generation of free radicals/ROS in skin were measured. The protection against ROS and the repair of damages caused by them can be realized with active antioxidants characterized by a high antioxidative power (AP). The effect of UV filter and antioxidants corresponding to their protection against free radicals/ROS in skin generated by UVAB irradiation can be quantified by the new radical sun protection factor (RSF). The RSF indicates the increase of time for staying in the sun to generate the same number of free radicals/ROS in the skin like for the unprotected skin. Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation. |
| doi_str_mv | 10.1016/j.saa.2005.10.013 |
| format | Article |
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nm) so that the spatial distribution of free radicals reaches up to the lower side of the dermis. In vivo measurements on human skin were performed with a L-band ESR spectrometer and a surface coil integrating the signal intensities from all skin layers to get a sufficient signal amplitude. Using this experimental arrangement the protection of UVB and UVA/B filter against the generation of free radicals/ROS in skin were measured. The protection against ROS and the repair of damages caused by them can be realized with active antioxidants characterized by a high antioxidative power (AP). The effect of UV filter and antioxidants corresponding to their protection against free radicals/ROS in skin generated by UVAB irradiation can be quantified by the new radical sun protection factor (RSF). The RSF indicates the increase of time for staying in the sun to generate the same number of free radicals/ROS in the skin like for the unprotected skin. Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation.</description><identifier>ISSN: 1386-1425</identifier><identifier>DOI: 10.1016/j.saa.2005.10.013</identifier><identifier>PMID: 16543118</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Antioxidants - pharmacology ; Biopsy ; Cyclic N-Oxides - chemistry ; Cyclic N-Oxides - metabolism ; Electron Spin Resonance Spectroscopy ; ESR spectroscopy ; Free radical ; Free Radicals - metabolism ; Humans ; Pyrrolidines - chemistry ; Pyrrolidines - metabolism ; Reactive Oxygen Species - metabolism ; Skin ; Skin - drug effects ; Skin - radiation effects ; Spin Labels ; Spin Trapping ; Sunscreening Agents - pharmacology ; Swine ; Ultraviolet Rays ; UV irradiation</subject><ispartof>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2006-03, Vol.63 (4), p.840-845</ispartof><rights>2005 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-62b3b2ab7ecf87cb7c30d8235dd6eb2c49e466f5a92c5dc6fc9693ff85c0455d3</citedby><cites>FETCH-LOGICAL-c351t-62b3b2ab7ecf87cb7c30d8235dd6eb2c49e466f5a92c5dc6fc9693ff85c0455d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1386142505005639$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16543118$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herrling, Th</creatorcontrib><creatorcontrib>Jung, K.</creatorcontrib><creatorcontrib>Fuchs, J.</creatorcontrib><title>Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin</title><title>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</title><addtitle>Spectrochim Acta A Mol Biomol Spectrosc</addtitle><description>Free radicals/reactive oxygen species (ROS) generated in skin by UV irradiation were measured by electron spin resonance (ESR). To increase the sensitivity of measurement the short life free radicals/ROS were scavenged and accumulated by using the nitroxyl probe 3-carboxy-2,2,5,5-tetrametylpyrrolidine-1-oxyl (PCA). The spatial distribution of free radicals/ROS measured in pig skin biopsies with ESR imaging after UV irradiation corresponds to the intensity decay of irradiance in the depth of the skin. The main part of free radicals/ROS were generated by UVA (320–400
nm) so that the spatial distribution of free radicals reaches up to the lower side of the dermis. In vivo measurements on human skin were performed with a L-band ESR spectrometer and a surface coil integrating the signal intensities from all skin layers to get a sufficient signal amplitude. Using this experimental arrangement the protection of UVB and UVA/B filter against the generation of free radicals/ROS in skin were measured. The protection against ROS and the repair of damages caused by them can be realized with active antioxidants characterized by a high antioxidative power (AP). The effect of UV filter and antioxidants corresponding to their protection against free radicals/ROS in skin generated by UVAB irradiation can be quantified by the new radical sun protection factor (RSF). The RSF indicates the increase of time for staying in the sun to generate the same number of free radicals/ROS in the skin like for the unprotected skin. Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Biopsy</subject><subject>Cyclic N-Oxides - chemistry</subject><subject>Cyclic N-Oxides - metabolism</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>ESR spectroscopy</subject><subject>Free radical</subject><subject>Free Radicals - metabolism</subject><subject>Humans</subject><subject>Pyrrolidines - chemistry</subject><subject>Pyrrolidines - metabolism</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Skin</subject><subject>Skin - drug effects</subject><subject>Skin - radiation effects</subject><subject>Spin Labels</subject><subject>Spin Trapping</subject><subject>Sunscreening Agents - pharmacology</subject><subject>Swine</subject><subject>Ultraviolet Rays</subject><subject>UV irradiation</subject><issn>1386-1425</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD1PwzAQhj2AaCn8ABbkCcGQ1o5jJxETqviSQEVAWS3HPiOXJil2UtF_j6tWYmM63el5X-kehM4oGVNCxWQxDkqNU0J43MeEsgM0pKwQCc1SPkDHISwIIbRIyREaUMEzRmkxRC_PoELvoYamC7i1eP6RfEIDXnVgsPUA2CvjtFqGiQelO7cG3P5sIoPDCrSDgC9fZ29X2MXDl2tO0KGNMJzu5wjN727fpw_J0-z-cXrzlGjGaZeItGJVqqoctC1yXeWaEVOkjBsjoEp1VkImhOWqTDU3WlhdipJZW3BNMs4NG6GLXe_Kt989hE7WLmhYLlUDbR-kyHNeCFJEkO5A7dsQPFi58q5WfiMpkVt1ciGjOrlVtz1FdTFzvi_vqxrMX2LvLQLXOwDii2sHXoaootFgnAfdSdO6f-p_AScngPg</recordid><startdate>20060313</startdate><enddate>20060313</enddate><creator>Herrling, Th</creator><creator>Jung, K.</creator><creator>Fuchs, J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060313</creationdate><title>Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin</title><author>Herrling, Th ; Jung, K. ; Fuchs, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-62b3b2ab7ecf87cb7c30d8235dd6eb2c49e466f5a92c5dc6fc9693ff85c0455d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Biopsy</topic><topic>Cyclic N-Oxides - chemistry</topic><topic>Cyclic N-Oxides - metabolism</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>ESR spectroscopy</topic><topic>Free radical</topic><topic>Free Radicals - metabolism</topic><topic>Humans</topic><topic>Pyrrolidines - chemistry</topic><topic>Pyrrolidines - metabolism</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Skin</topic><topic>Skin - drug effects</topic><topic>Skin - radiation effects</topic><topic>Spin Labels</topic><topic>Spin Trapping</topic><topic>Sunscreening Agents - pharmacology</topic><topic>Swine</topic><topic>Ultraviolet Rays</topic><topic>UV irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herrling, Th</creatorcontrib><creatorcontrib>Jung, K.</creatorcontrib><creatorcontrib>Fuchs, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herrling, Th</au><au>Jung, K.</au><au>Fuchs, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin</atitle><jtitle>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy</jtitle><addtitle>Spectrochim Acta A Mol Biomol Spectrosc</addtitle><date>2006-03-13</date><risdate>2006</risdate><volume>63</volume><issue>4</issue><spage>840</spage><epage>845</epage><pages>840-845</pages><issn>1386-1425</issn><abstract>Free radicals/reactive oxygen species (ROS) generated in skin by UV irradiation were measured by electron spin resonance (ESR). To increase the sensitivity of measurement the short life free radicals/ROS were scavenged and accumulated by using the nitroxyl probe 3-carboxy-2,2,5,5-tetrametylpyrrolidine-1-oxyl (PCA). The spatial distribution of free radicals/ROS measured in pig skin biopsies with ESR imaging after UV irradiation corresponds to the intensity decay of irradiance in the depth of the skin. The main part of free radicals/ROS were generated by UVA (320–400
nm) so that the spatial distribution of free radicals reaches up to the lower side of the dermis. In vivo measurements on human skin were performed with a L-band ESR spectrometer and a surface coil integrating the signal intensities from all skin layers to get a sufficient signal amplitude. Using this experimental arrangement the protection of UVB and UVA/B filter against the generation of free radicals/ROS in skin were measured. The protection against ROS and the repair of damages caused by them can be realized with active antioxidants characterized by a high antioxidative power (AP). The effect of UV filter and antioxidants corresponding to their protection against free radicals/ROS in skin generated by UVAB irradiation can be quantified by the new radical sun protection factor (RSF). The RSF indicates the increase of time for staying in the sun to generate the same number of free radicals/ROS in the skin like for the unprotected skin. Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>16543118</pmid><doi>10.1016/j.saa.2005.10.013</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Antioxidants - pharmacology Biopsy Cyclic N-Oxides - chemistry Cyclic N-Oxides - metabolism Electron Spin Resonance Spectroscopy ESR spectroscopy Free radical Free Radicals - metabolism Humans Pyrrolidines - chemistry Pyrrolidines - metabolism Reactive Oxygen Species - metabolism Skin Skin - drug effects Skin - radiation effects Spin Labels Spin Trapping Sunscreening Agents - pharmacology Swine Ultraviolet Rays UV irradiation |
| title | Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin |
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