Nuclear Pore Components Are Involved in the Transcriptional Regulation of Dosage Compensation in Drosophila
Dosage compensation in Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from Drosophila embryos, Schneider cells, and hu...
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| Veröffentlicht in: | Molecular cell 2006-03, Vol.21 (6), p.811-823 |
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| creator | Mendjan, Sascha Taipale, Mikko Kind, Jop Holz, Herbert Gebhardt, Philipp Schelder, Malgorzata Vermeulen, Michiel Buscaino, Alessia Duncan, Kent Mueller, Juerg Wilm, Matthias Stunnenberg, Henk G. Saumweber, Harald Akhtar, Asifa |
| description | Dosage compensation in
Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from
Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in
Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes. |
| doi_str_mv | 10.1016/j.molcel.2006.02.007 |
| format | Article |
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Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from
Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in
Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.</description><identifier>ISSN: 1097-2765</identifier><identifier>EISSN: 1097-4164</identifier><identifier>DOI: 10.1016/j.molcel.2006.02.007</identifier><identifier>PMID: 16543150</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetyltransferases ; Animals ; Animals, Genetically Modified ; Cell Line ; Chromatography, Affinity ; DEVBIO ; DNA ; Dosage Compensation, Genetic ; Drosophila ; Drosophila - embryology ; Drosophila - genetics ; Drosophila - metabolism ; Drosophila Proteins - chemistry ; Drosophila Proteins - metabolism ; Eucarya ; Evolution, Molecular ; Female ; Gene Expression Regulation ; HeLa Cells ; Humans ; Male ; Mass Spectrometry ; Nuclear Pore - metabolism ; Nuclear Pore Complex Proteins - metabolism ; Nuclear Pore Complex Proteins - physiology ; Nuclear Proteins - chemistry ; Nuclear Proteins - metabolism ; Protein Kinases - metabolism ; Protein Kinases - physiology ; TOR Serine-Threonine Kinases ; Transcription Factors - chemistry ; Transcription Factors - metabolism ; Transcription, Genetic ; X Chromosome - genetics</subject><ispartof>Molecular cell, 2006-03, Vol.21 (6), p.811-823</ispartof><rights>2006 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-3e8cf0b35a34a184f6977463f13be15eb6703ad0e80b2957ab4e459cb436c8fc3</citedby><cites>FETCH-LOGICAL-c503t-3e8cf0b35a34a184f6977463f13be15eb6703ad0e80b2957ab4e459cb436c8fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.molcel.2006.02.007$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16543150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mendjan, Sascha</creatorcontrib><creatorcontrib>Taipale, Mikko</creatorcontrib><creatorcontrib>Kind, Jop</creatorcontrib><creatorcontrib>Holz, Herbert</creatorcontrib><creatorcontrib>Gebhardt, Philipp</creatorcontrib><creatorcontrib>Schelder, Malgorzata</creatorcontrib><creatorcontrib>Vermeulen, Michiel</creatorcontrib><creatorcontrib>Buscaino, Alessia</creatorcontrib><creatorcontrib>Duncan, Kent</creatorcontrib><creatorcontrib>Mueller, Juerg</creatorcontrib><creatorcontrib>Wilm, Matthias</creatorcontrib><creatorcontrib>Stunnenberg, Henk G.</creatorcontrib><creatorcontrib>Saumweber, Harald</creatorcontrib><creatorcontrib>Akhtar, Asifa</creatorcontrib><title>Nuclear Pore Components Are Involved in the Transcriptional Regulation of Dosage Compensation in Drosophila</title><title>Molecular cell</title><addtitle>Mol Cell</addtitle><description>Dosage compensation in
Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from
Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in
Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.</description><subject>Acetyltransferases</subject><subject>Animals</subject><subject>Animals, Genetically Modified</subject><subject>Cell Line</subject><subject>Chromatography, Affinity</subject><subject>DEVBIO</subject><subject>DNA</subject><subject>Dosage Compensation, Genetic</subject><subject>Drosophila</subject><subject>Drosophila - embryology</subject><subject>Drosophila - genetics</subject><subject>Drosophila - metabolism</subject><subject>Drosophila Proteins - chemistry</subject><subject>Drosophila Proteins - metabolism</subject><subject>Eucarya</subject><subject>Evolution, Molecular</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Male</subject><subject>Mass Spectrometry</subject><subject>Nuclear Pore - metabolism</subject><subject>Nuclear Pore Complex Proteins - metabolism</subject><subject>Nuclear Pore Complex Proteins - physiology</subject><subject>Nuclear Proteins - chemistry</subject><subject>Nuclear Proteins - metabolism</subject><subject>Protein Kinases - metabolism</subject><subject>Protein Kinases - physiology</subject><subject>TOR Serine-Threonine Kinases</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>X Chromosome - genetics</subject><issn>1097-2765</issn><issn>1097-4164</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAYhYso7rr6D0Ry5d3UN81XeyMss34sLCqyXoc0fbubMU1q0g74783QAe8UAskbnnNCzqmq1xRqClS-O9RT9BZ93QDIGpoaQD2pLil0asep5E_P50ZJcVG9yPkAQLlou-fVBZWCMyrgsvr5ZbUeTSLfYkKyj9McA4Ylk-sy3oZj9EcciAtkeURyn0zINrl5cTEYT77jw-rNaSBxJDcxm4fNA0PerovwJsUc50fnzcvq2Wh8xlfn_ar68fHD_f7z7u7rp9v99d3OCmDLjmFrR-iZMIwb2vJRdkpxyUbKeqQCe6mAmQGwhb7phDI9Ry4623MmbTtadlW93XznFH-tmBc9uVyS8iZgXLOWSglZ1n_BBhgILkQB-Qba8pmccNRzcpNJvzUFfWpDH_TWhj61oaHRpY0ie3P2X_sJh7-ic_wFeL8BWOI4Okw6W4fB4uAS2kUP0f37hT_XQp4h</recordid><startdate>20060317</startdate><enddate>20060317</enddate><creator>Mendjan, Sascha</creator><creator>Taipale, Mikko</creator><creator>Kind, Jop</creator><creator>Holz, Herbert</creator><creator>Gebhardt, Philipp</creator><creator>Schelder, Malgorzata</creator><creator>Vermeulen, Michiel</creator><creator>Buscaino, Alessia</creator><creator>Duncan, Kent</creator><creator>Mueller, Juerg</creator><creator>Wilm, Matthias</creator><creator>Stunnenberg, Henk G.</creator><creator>Saumweber, Harald</creator><creator>Akhtar, Asifa</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20060317</creationdate><title>Nuclear Pore Components Are Involved in the Transcriptional Regulation of Dosage Compensation in Drosophila</title><author>Mendjan, Sascha ; 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Drosophila is dependent on MSL proteins and involves hypertranscription of the male X chromosome, which ensures equal X-linked gene expression in both sexes. Here, we report the purification of enzymatically active MSL complexes from
Drosophila embryos, Schneider cells, and human HeLa cells. We find a stable association of the histone H4 lysine 16-specific acetyltransferase MOF with the RNA/protein containing MSL complex as well as with an evolutionary conserved complex. We show that the MSL complex interacts with several components of the nuclear pore, in particular Mtor/TPR and Nup153. Strikingly, knockdown of Mtor or Nup153 results in loss of the typical MSL X-chromosomal staining and dosage compensation in
Drosophila male cells but not in female cells. These results reveal an unexpected physical and functional connection between nuclear pore components and chromatin regulation through MSL proteins, highlighting the role of nucleoporins in gene regulation in higher eukaryotes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16543150</pmid><doi>10.1016/j.molcel.2006.02.007</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetyltransferases Animals Animals, Genetically Modified Cell Line Chromatography, Affinity DEVBIO DNA Dosage Compensation, Genetic Drosophila Drosophila - embryology Drosophila - genetics Drosophila - metabolism Drosophila Proteins - chemistry Drosophila Proteins - metabolism Eucarya Evolution, Molecular Female Gene Expression Regulation HeLa Cells Humans Male Mass Spectrometry Nuclear Pore - metabolism Nuclear Pore Complex Proteins - metabolism Nuclear Pore Complex Proteins - physiology Nuclear Proteins - chemistry Nuclear Proteins - metabolism Protein Kinases - metabolism Protein Kinases - physiology TOR Serine-Threonine Kinases Transcription Factors - chemistry Transcription Factors - metabolism Transcription, Genetic X Chromosome - genetics |
| title | Nuclear Pore Components Are Involved in the Transcriptional Regulation of Dosage Compensation in Drosophila |
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