Correction of Hunter syndrome in the MPSII mouse model by AAV2/8-mediated gene delivery
Mucopolysaccharidosis type II (MPSII; Hunter syndrome) is a lysosomal storage disorder caused by a deficiency in the enzyme iduronate 2-sulfatase (IDS). At present, the therapeutic approaches for MPSII are enzyme replacement therapy and bone marrow transplantation, although these therapies have some...
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creator | Cardone, Monica Polito, Vinicia Assunta Pepe, Stefano Mann, Linda D'Azzo, Alessandra Auricchio, Alberto Ballabio, Andrea Cosma, Maria Pia |
description | Mucopolysaccharidosis type II (MPSII; Hunter syndrome) is a lysosomal storage disorder caused by a deficiency in the enzyme iduronate 2-sulfatase (IDS). At present, the therapeutic approaches for MPSII are enzyme replacement therapy and bone marrow transplantation, although these therapies have some limitations. The availability of new AAV serotypes that display tissue-specific tropism and promote sustained expression of transgenes offers the possibility of AAV-mediated gene therapy for the systemic treatment of lysosomal diseases, including MPSII. We have characterized in detail the phenotype of IDS-deficient mice, a model of human MPSII. These mice display a progressive accumulation of glycosaminoglycans (GAGs) in many organs and excessive excretion of these compounds in their urine. Furthermore, they develop skeleton deformities, particularly of the craniofacial bones, and alopecia, they perform poorly in open-field tests and they have a severely compromised walking pattern. In addition, they present neuropathological defects. We have designed an efficient gene therapy approach for the treatment of these MPSII mice. AAV2/8TBG-IDS viral particles were administrated intravenously to adult MPSII mice. The plasma and tissue IDS activities were completely restored in all of the treated mice. This rescue of the enzymatic activity resulted in the full clearance of the accumulated GAGs in all of the tissues analyzed, the normalization of the GAG levels in the urine and the correction of the skeleton malformations. Overall, our findings suggest that this in vivo gene transfer approach has potential for the systemic treatment of patients with Hunter syndrome. |
doi_str_mv | 10.1093/hmg/ddl038 |
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At present, the therapeutic approaches for MPSII are enzyme replacement therapy and bone marrow transplantation, although these therapies have some limitations. The availability of new AAV serotypes that display tissue-specific tropism and promote sustained expression of transgenes offers the possibility of AAV-mediated gene therapy for the systemic treatment of lysosomal diseases, including MPSII. We have characterized in detail the phenotype of IDS-deficient mice, a model of human MPSII. These mice display a progressive accumulation of glycosaminoglycans (GAGs) in many organs and excessive excretion of these compounds in their urine. Furthermore, they develop skeleton deformities, particularly of the craniofacial bones, and alopecia, they perform poorly in open-field tests and they have a severely compromised walking pattern. In addition, they present neuropathological defects. We have designed an efficient gene therapy approach for the treatment of these MPSII mice. AAV2/8TBG-IDS viral particles were administrated intravenously to adult MPSII mice. The plasma and tissue IDS activities were completely restored in all of the treated mice. This rescue of the enzymatic activity resulted in the full clearance of the accumulated GAGs in all of the tissues analyzed, the normalization of the GAG levels in the urine and the correction of the skeleton malformations. Overall, our findings suggest that this in vivo gene transfer approach has potential for the systemic treatment of patients with Hunter syndrome.</description><identifier>ISSN: 0964-6906</identifier><identifier>EISSN: 1460-2083</identifier><identifier>DOI: 10.1093/hmg/ddl038</identifier><identifier>PMID: 16505002</identifier><identifier>CODEN: HNGEE5</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adeno-associated virus ; Animals ; Biological and medical sciences ; Carbohydrates (enzymatic deficiencies). Glycogenosis ; Dependovirus - genetics ; Dependovirus - metabolism ; Disease Models, Animal ; Errors of metabolism ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Genetic Therapy - methods ; Genetic Vectors - genetics ; Genetic Vectors - metabolism ; Genetic Vectors - therapeutic use ; Genetics of eukaryotes. Biological and molecular evolution ; Glycosaminoglycans - metabolism ; Glycosaminoglycans - urine ; Humans ; Iduronate Sulfatase - blood ; Iduronate Sulfatase - genetics ; Iduronate Sulfatase - metabolism ; Kidney - cytology ; Kidney - metabolism ; Liver - cytology ; Liver - metabolism ; Lysosomes - metabolism ; Medical sciences ; Metabolic diseases ; Mice ; Mice, Knockout ; Molecular and cellular biology ; Motor Activity ; Mucopolysaccharidosis II - metabolism ; Mucopolysaccharidosis II - therapy ; Spleen - cytology ; Spleen - metabolism ; Thyroxine-Binding Proteins - genetics ; Thyroxine-Binding Proteins - metabolism</subject><ispartof>Human molecular genetics, 2006-04, Vol.15 (7), p.1225-1236</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Oxford University Press(England) Apr 1, 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c513t-1ee7d8f790be39c1036d50b361b1abf31bda6785a0d71ddf611ed9c96101de203</citedby><cites>FETCH-LOGICAL-c513t-1ee7d8f790be39c1036d50b361b1abf31bda6785a0d71ddf611ed9c96101de203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17674572$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16505002$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cardone, Monica</creatorcontrib><creatorcontrib>Polito, Vinicia Assunta</creatorcontrib><creatorcontrib>Pepe, Stefano</creatorcontrib><creatorcontrib>Mann, Linda</creatorcontrib><creatorcontrib>D'Azzo, Alessandra</creatorcontrib><creatorcontrib>Auricchio, Alberto</creatorcontrib><creatorcontrib>Ballabio, Andrea</creatorcontrib><creatorcontrib>Cosma, Maria Pia</creatorcontrib><title>Correction of Hunter syndrome in the MPSII mouse model by AAV2/8-mediated gene delivery</title><title>Human molecular genetics</title><addtitle>Hum. Mol. Genet</addtitle><description>Mucopolysaccharidosis type II (MPSII; Hunter syndrome) is a lysosomal storage disorder caused by a deficiency in the enzyme iduronate 2-sulfatase (IDS). At present, the therapeutic approaches for MPSII are enzyme replacement therapy and bone marrow transplantation, although these therapies have some limitations. The availability of new AAV serotypes that display tissue-specific tropism and promote sustained expression of transgenes offers the possibility of AAV-mediated gene therapy for the systemic treatment of lysosomal diseases, including MPSII. We have characterized in detail the phenotype of IDS-deficient mice, a model of human MPSII. These mice display a progressive accumulation of glycosaminoglycans (GAGs) in many organs and excessive excretion of these compounds in their urine. Furthermore, they develop skeleton deformities, particularly of the craniofacial bones, and alopecia, they perform poorly in open-field tests and they have a severely compromised walking pattern. In addition, they present neuropathological defects. We have designed an efficient gene therapy approach for the treatment of these MPSII mice. AAV2/8TBG-IDS viral particles were administrated intravenously to adult MPSII mice. The plasma and tissue IDS activities were completely restored in all of the treated mice. This rescue of the enzymatic activity resulted in the full clearance of the accumulated GAGs in all of the tissues analyzed, the normalization of the GAG levels in the urine and the correction of the skeleton malformations. Overall, our findings suggest that this in vivo gene transfer approach has potential for the systemic treatment of patients with Hunter syndrome.</description><subject>Adeno-associated virus</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates (enzymatic deficiencies). Glycogenosis</subject><subject>Dependovirus - genetics</subject><subject>Dependovirus - metabolism</subject><subject>Disease Models, Animal</subject><subject>Errors of metabolism</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Therapy - methods</subject><subject>Genetic Vectors - genetics</subject><subject>Genetic Vectors - metabolism</subject><subject>Genetic Vectors - therapeutic use</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Glycosaminoglycans - urine</subject><subject>Humans</subject><subject>Iduronate Sulfatase - blood</subject><subject>Iduronate Sulfatase - genetics</subject><subject>Iduronate Sulfatase - metabolism</subject><subject>Kidney - cytology</subject><subject>Kidney - metabolism</subject><subject>Liver - cytology</subject><subject>Liver - metabolism</subject><subject>Lysosomes - metabolism</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Molecular and cellular biology</subject><subject>Motor Activity</subject><subject>Mucopolysaccharidosis II - metabolism</subject><subject>Mucopolysaccharidosis II - therapy</subject><subject>Spleen - cytology</subject><subject>Spleen - metabolism</subject><subject>Thyroxine-Binding Proteins - genetics</subject><subject>Thyroxine-Binding Proteins - metabolism</subject><issn>0964-6906</issn><issn>1460-2083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1v1DAQBmALgei2cOEHIAuJHpDSnYkTOz6uVpTdsgUkCkVcLCeetCn5KHaC2H9fl11RiQsX-zCPXnn8MvYC4QRBi_l1dzV3rgVRPGIzzCQkKRTiMZuBllkiNcgDdhjCDQDKTKin7ABlDjlAOmOXy8F7qsZm6PlQ89XUj-R52PbODx3xpufjNfHzT5_Xa94NU6B4Omp5ueWLxdd0XiQducaO5PgV9cTjrPlFfvuMPaltG-j5_j5iX07fXixXyebju_VysUmqHMWYIJFyRa00lCR0hSCky6EUEku0ZS2wdFaqIrfgFDpXS0RyutISAR2lII7Y8S731g8_Jwqj6ZpQUdvanuJzjVQqz7X-P0whRY1_El_9A2-GyfdxCZMiiqhEGtGbHar8EIKn2tz6prN-axDMfSkmlmJ2pUT8cp84lfG3Hui-hQhe74ENlW1rb_uqCQ9OSZXl6t4lO9eEkX7_nVv_I-4pVG5W376bi8sPm83p-8yciTt6zKKK</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Cardone, Monica</creator><creator>Polito, Vinicia Assunta</creator><creator>Pepe, Stefano</creator><creator>Mann, Linda</creator><creator>D'Azzo, Alessandra</creator><creator>Auricchio, Alberto</creator><creator>Ballabio, Andrea</creator><creator>Cosma, Maria Pia</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Correction of Hunter syndrome in the MPSII mouse model by AAV2/8-mediated gene delivery</title><author>Cardone, Monica ; Polito, Vinicia Assunta ; Pepe, Stefano ; Mann, Linda ; D'Azzo, Alessandra ; Auricchio, Alberto ; Ballabio, Andrea ; Cosma, Maria Pia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-1ee7d8f790be39c1036d50b361b1abf31bda6785a0d71ddf611ed9c96101de203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adeno-associated virus</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates (enzymatic deficiencies). Glycogenosis</topic><topic>Dependovirus - genetics</topic><topic>Dependovirus - metabolism</topic><topic>Disease Models, Animal</topic><topic>Errors of metabolism</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Therapy - methods</topic><topic>Genetic Vectors - genetics</topic><topic>Genetic Vectors - metabolism</topic><topic>Genetic Vectors - therapeutic use</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Glycosaminoglycans - urine</topic><topic>Humans</topic><topic>Iduronate Sulfatase - blood</topic><topic>Iduronate Sulfatase - genetics</topic><topic>Iduronate Sulfatase - metabolism</topic><topic>Kidney - cytology</topic><topic>Kidney - metabolism</topic><topic>Liver - cytology</topic><topic>Liver - metabolism</topic><topic>Lysosomes - metabolism</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Molecular and cellular biology</topic><topic>Motor Activity</topic><topic>Mucopolysaccharidosis II - metabolism</topic><topic>Mucopolysaccharidosis II - therapy</topic><topic>Spleen - cytology</topic><topic>Spleen - metabolism</topic><topic>Thyroxine-Binding Proteins - genetics</topic><topic>Thyroxine-Binding Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardone, Monica</creatorcontrib><creatorcontrib>Polito, Vinicia Assunta</creatorcontrib><creatorcontrib>Pepe, Stefano</creatorcontrib><creatorcontrib>Mann, Linda</creatorcontrib><creatorcontrib>D'Azzo, Alessandra</creatorcontrib><creatorcontrib>Auricchio, Alberto</creatorcontrib><creatorcontrib>Ballabio, Andrea</creatorcontrib><creatorcontrib>Cosma, Maria Pia</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Human molecular genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cardone, Monica</au><au>Polito, Vinicia Assunta</au><au>Pepe, Stefano</au><au>Mann, Linda</au><au>D'Azzo, Alessandra</au><au>Auricchio, Alberto</au><au>Ballabio, Andrea</au><au>Cosma, Maria Pia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correction of Hunter syndrome in the MPSII mouse model by AAV2/8-mediated gene delivery</atitle><jtitle>Human molecular genetics</jtitle><addtitle>Hum. Mol. Genet</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>15</volume><issue>7</issue><spage>1225</spage><epage>1236</epage><pages>1225-1236</pages><issn>0964-6906</issn><eissn>1460-2083</eissn><coden>HNGEE5</coden><abstract>Mucopolysaccharidosis type II (MPSII; Hunter syndrome) is a lysosomal storage disorder caused by a deficiency in the enzyme iduronate 2-sulfatase (IDS). At present, the therapeutic approaches for MPSII are enzyme replacement therapy and bone marrow transplantation, although these therapies have some limitations. The availability of new AAV serotypes that display tissue-specific tropism and promote sustained expression of transgenes offers the possibility of AAV-mediated gene therapy for the systemic treatment of lysosomal diseases, including MPSII. We have characterized in detail the phenotype of IDS-deficient mice, a model of human MPSII. These mice display a progressive accumulation of glycosaminoglycans (GAGs) in many organs and excessive excretion of these compounds in their urine. Furthermore, they develop skeleton deformities, particularly of the craniofacial bones, and alopecia, they perform poorly in open-field tests and they have a severely compromised walking pattern. In addition, they present neuropathological defects. We have designed an efficient gene therapy approach for the treatment of these MPSII mice. AAV2/8TBG-IDS viral particles were administrated intravenously to adult MPSII mice. The plasma and tissue IDS activities were completely restored in all of the treated mice. This rescue of the enzymatic activity resulted in the full clearance of the accumulated GAGs in all of the tissues analyzed, the normalization of the GAG levels in the urine and the correction of the skeleton malformations. Overall, our findings suggest that this in vivo gene transfer approach has potential for the systemic treatment of patients with Hunter syndrome.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>16505002</pmid><doi>10.1093/hmg/ddl038</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
subjects | Adeno-associated virus Animals Biological and medical sciences Carbohydrates (enzymatic deficiencies). Glycogenosis Dependovirus - genetics Dependovirus - metabolism Disease Models, Animal Errors of metabolism Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genetic Therapy - methods Genetic Vectors - genetics Genetic Vectors - metabolism Genetic Vectors - therapeutic use Genetics of eukaryotes. Biological and molecular evolution Glycosaminoglycans - metabolism Glycosaminoglycans - urine Humans Iduronate Sulfatase - blood Iduronate Sulfatase - genetics Iduronate Sulfatase - metabolism Kidney - cytology Kidney - metabolism Liver - cytology Liver - metabolism Lysosomes - metabolism Medical sciences Metabolic diseases Mice Mice, Knockout Molecular and cellular biology Motor Activity Mucopolysaccharidosis II - metabolism Mucopolysaccharidosis II - therapy Spleen - cytology Spleen - metabolism Thyroxine-Binding Proteins - genetics Thyroxine-Binding Proteins - metabolism |
title | Correction of Hunter syndrome in the MPSII mouse model by AAV2/8-mediated gene delivery |
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