Inhibition of mRNA deadenylation and degradation by different types of cell stress
We have previously observed rapid and strong inhibition of mRNA deadenylation and degradation in response to UV-B light [Gowrishankar et al., Biol. Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all shor...
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| Veröffentlicht in: | Biological chemistry 2006-03, Vol.387 (3), p.323-327 |
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| creator | Gowrishankar, Gayatri Winzen, Reinhard Dittrich-Breiholz, Oliver Redich, Natalie Kracht, Michael Holtmann, Helmut |
| description | We have previously observed rapid and strong inhibition of mRNA deadenylation and degradation in response to UV-B light [Gowrishankar et al., Biol. Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all short-lived mRNAs assayed. Stabilization was observed in HeLa cells, as well as in the keratinocyte line HaCaT. It affected constitutively expressed mRNA species, as well as species induced by the inflammatory cytokine IL-1. Many of the latter encode proteins involved in inflammation, suggesting that stress-induced inhibition of mRNA deadenylation contributes to changes in inflammatory gene expression. Deadenylation and degradation of tet-off-expressed mRNAs were also inhibited upon exposure to H2O2. However, scavengers of reactive oxygen species did not interfere with UV-B-induced inhibition of degradation, arguing against the involvement of UV-induced H2O2 in these effects of UV-B light. Heat shock and hyperosmolarity also inhibited mRNA deadenylation and degradation, whereas γ-radiation did not. Thus, inhibition of mRNA deadenylation and degradation is a cellular response elicited by several but not all inducers of cell stress. |
| doi_str_mv | 10.1515/BC.2006.043 |
| format | Article |
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Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all short-lived mRNAs assayed. Stabilization was observed in HeLa cells, as well as in the keratinocyte line HaCaT. It affected constitutively expressed mRNA species, as well as species induced by the inflammatory cytokine IL-1. Many of the latter encode proteins involved in inflammation, suggesting that stress-induced inhibition of mRNA deadenylation contributes to changes in inflammatory gene expression. Deadenylation and degradation of tet-off-expressed mRNAs were also inhibited upon exposure to H2O2. However, scavengers of reactive oxygen species did not interfere with UV-B-induced inhibition of degradation, arguing against the involvement of UV-induced H2O2 in these effects of UV-B light. Heat shock and hyperosmolarity also inhibited mRNA deadenylation and degradation, whereas γ-radiation did not. Thus, inhibition of mRNA deadenylation and degradation is a cellular response elicited by several but not all inducers of cell stress.</description><identifier>ISSN: 1431-6730</identifier><identifier>EISSN: 1437-4315</identifier><identifier>DOI: 10.1515/BC.2006.043</identifier><identifier>PMID: 16542155</identifier><language>eng</language><publisher>Germany: Walter de Gruyter</publisher><subject>Adenine - metabolism ; cytokines ; Cytokines - metabolism ; Electrophoresis, Polyacrylamide Gel ; Gene Expression - radiation effects ; Heat-Shock Response ; HeLa Cells - metabolism ; HeLa Cells - radiation effects ; Humans ; Hydrogen Peroxide - metabolism ; Hydrogen Peroxide - pharmacology ; inflammation ; Inflammation - chemically induced ; Interleukin-1 - metabolism ; Keratinocytes - metabolism ; Keratinocytes - radiation effects ; mRNA stability ; Osmolar Concentration ; poly(A)-tail ; RNA, Messenger - antagonists & inhibitors ; RNA, Messenger - metabolism ; RNA, Messenger - radiation effects ; stress ; Temperature ; Time Factors ; Ultraviolet Rays ; UV light</subject><ispartof>Biological chemistry, 2006-03, Vol.387 (3), p.323-327</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-3ade53999b3a75978d115c2cb257889a2d9c6bded4cfa2af4010fd1cc72b1b4d3</citedby><cites>FETCH-LOGICAL-c422t-3ade53999b3a75978d115c2cb257889a2d9c6bded4cfa2af4010fd1cc72b1b4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16542155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gowrishankar, Gayatri</creatorcontrib><creatorcontrib>Winzen, Reinhard</creatorcontrib><creatorcontrib>Dittrich-Breiholz, Oliver</creatorcontrib><creatorcontrib>Redich, Natalie</creatorcontrib><creatorcontrib>Kracht, Michael</creatorcontrib><creatorcontrib>Holtmann, Helmut</creatorcontrib><title>Inhibition of mRNA deadenylation and degradation by different types of cell stress</title><title>Biological chemistry</title><addtitle>Biological Chemistry</addtitle><description>We have previously observed rapid and strong inhibition of mRNA deadenylation and degradation in response to UV-B light [Gowrishankar et al., Biol. Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all short-lived mRNAs assayed. Stabilization was observed in HeLa cells, as well as in the keratinocyte line HaCaT. It affected constitutively expressed mRNA species, as well as species induced by the inflammatory cytokine IL-1. Many of the latter encode proteins involved in inflammation, suggesting that stress-induced inhibition of mRNA deadenylation contributes to changes in inflammatory gene expression. Deadenylation and degradation of tet-off-expressed mRNAs were also inhibited upon exposure to H2O2. However, scavengers of reactive oxygen species did not interfere with UV-B-induced inhibition of degradation, arguing against the involvement of UV-induced H2O2 in these effects of UV-B light. Heat shock and hyperosmolarity also inhibited mRNA deadenylation and degradation, whereas γ-radiation did not. Thus, inhibition of mRNA deadenylation and degradation is a cellular response elicited by several but not all inducers of cell stress.</description><subject>Adenine - metabolism</subject><subject>cytokines</subject><subject>Cytokines - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gene Expression - radiation effects</subject><subject>Heat-Shock Response</subject><subject>HeLa Cells - metabolism</subject><subject>HeLa Cells - radiation effects</subject><subject>Humans</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>inflammation</subject><subject>Inflammation - chemically induced</subject><subject>Interleukin-1 - metabolism</subject><subject>Keratinocytes - metabolism</subject><subject>Keratinocytes - radiation effects</subject><subject>mRNA stability</subject><subject>Osmolar Concentration</subject><subject>poly(A)-tail</subject><subject>RNA, Messenger - antagonists & inhibitors</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Messenger - radiation effects</subject><subject>stress</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Ultraviolet Rays</subject><subject>UV light</subject><issn>1431-6730</issn><issn>1437-4315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAUhS0EoqUwsaNMLCjFzzgZ2_BopQpEVWC0HNuBQJMUO5XIv8dpKhiZru_x53OvDwDnCI4RQ-x6mo4xhNEYUnIAhogSHlKC2OHujMKIEzgAJ859QAhjDx2DAYoYxYixIVjOq_ciK5qiroI6D8rlwyTQRmpTtWu5U2WlvfJmpe77rA10kefGmqoJmnZjXPdQmfU6cI01zp2Co1yunTnb1xF4vrtdpbNw8Xg_TyeLUFGMm5D4IYwkSZIRyVnCY40QU1hlmPE4TiTWiYoybTRVucQypxDBXCOlOM5QRjUZgcved2Prr61xjSgL1-0hK1NvnYg4Zwz5D_8HYshiTlDiwaseVLZ2zppcbGxRStsKBEWXtZimostaeFdPX-xtt1lp9B-7D9cDYQ8UrjHfv_fSfvrdCGfiaUXFbPm6WtxMX0RKfgDc4Yim</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Gowrishankar, Gayatri</creator><creator>Winzen, Reinhard</creator><creator>Dittrich-Breiholz, Oliver</creator><creator>Redich, Natalie</creator><creator>Kracht, Michael</creator><creator>Holtmann, Helmut</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>Inhibition of mRNA deadenylation and degradation by different types of cell stress</title><author>Gowrishankar, Gayatri ; Winzen, Reinhard ; Dittrich-Breiholz, Oliver ; Redich, Natalie ; Kracht, Michael ; Holtmann, Helmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-3ade53999b3a75978d115c2cb257889a2d9c6bded4cfa2af4010fd1cc72b1b4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adenine - metabolism</topic><topic>cytokines</topic><topic>Cytokines - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Gene Expression - radiation effects</topic><topic>Heat-Shock Response</topic><topic>HeLa Cells - metabolism</topic><topic>HeLa Cells - radiation effects</topic><topic>Humans</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>inflammation</topic><topic>Inflammation - chemically induced</topic><topic>Interleukin-1 - metabolism</topic><topic>Keratinocytes - metabolism</topic><topic>Keratinocytes - radiation effects</topic><topic>mRNA stability</topic><topic>Osmolar Concentration</topic><topic>poly(A)-tail</topic><topic>RNA, Messenger - antagonists & inhibitors</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Messenger - radiation effects</topic><topic>stress</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Ultraviolet Rays</topic><topic>UV light</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gowrishankar, Gayatri</creatorcontrib><creatorcontrib>Winzen, Reinhard</creatorcontrib><creatorcontrib>Dittrich-Breiholz, Oliver</creatorcontrib><creatorcontrib>Redich, Natalie</creatorcontrib><creatorcontrib>Kracht, Michael</creatorcontrib><creatorcontrib>Holtmann, Helmut</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gowrishankar, Gayatri</au><au>Winzen, Reinhard</au><au>Dittrich-Breiholz, Oliver</au><au>Redich, Natalie</au><au>Kracht, Michael</au><au>Holtmann, Helmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of mRNA deadenylation and degradation by different types of cell stress</atitle><jtitle>Biological chemistry</jtitle><addtitle>Biological Chemistry</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>387</volume><issue>3</issue><spage>323</spage><epage>327</epage><pages>323-327</pages><issn>1431-6730</issn><eissn>1437-4315</eissn><abstract>We have previously observed rapid and strong inhibition of mRNA deadenylation and degradation in response to UV-B light [Gowrishankar et al., Biol. Chem. 386 (2005), pp. 1287–1293]. Expression analysis using a microarray for inflammatory genes showed that UV-B light induces stabilization of all short-lived mRNAs assayed. Stabilization was observed in HeLa cells, as well as in the keratinocyte line HaCaT. It affected constitutively expressed mRNA species, as well as species induced by the inflammatory cytokine IL-1. Many of the latter encode proteins involved in inflammation, suggesting that stress-induced inhibition of mRNA deadenylation contributes to changes in inflammatory gene expression. Deadenylation and degradation of tet-off-expressed mRNAs were also inhibited upon exposure to H2O2. However, scavengers of reactive oxygen species did not interfere with UV-B-induced inhibition of degradation, arguing against the involvement of UV-induced H2O2 in these effects of UV-B light. Heat shock and hyperosmolarity also inhibited mRNA deadenylation and degradation, whereas γ-radiation did not. 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| subjects | Adenine - metabolism cytokines Cytokines - metabolism Electrophoresis, Polyacrylamide Gel Gene Expression - radiation effects Heat-Shock Response HeLa Cells - metabolism HeLa Cells - radiation effects Humans Hydrogen Peroxide - metabolism Hydrogen Peroxide - pharmacology inflammation Inflammation - chemically induced Interleukin-1 - metabolism Keratinocytes - metabolism Keratinocytes - radiation effects mRNA stability Osmolar Concentration poly(A)-tail RNA, Messenger - antagonists & inhibitors RNA, Messenger - metabolism RNA, Messenger - radiation effects stress Temperature Time Factors Ultraviolet Rays UV light |
| title | Inhibition of mRNA deadenylation and degradation by different types of cell stress |
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