Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation
During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well‐understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies we...
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| Veröffentlicht in: | American journal of transplantation 2006-04, Vol.6 (4), p.806-824 |
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| creator | Borozan, I. Chen, L. Sun, J. Tannis, L. ‐L. Guindi, M. Rotstein, O. D. Heathcote, J. Edwards, A. M. Grant, D. McGilvray, I. D. |
| description | During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well‐understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies were taken early in the donor operation (OPENING), after transection of the donor liver (PRECLAMP) and following implantation of the graft (post hepatic artery, [PHA]); these were evaluated for histology, tissue glutathione content and gene expression using a 19K‐human cDNA microarray. LDLT was associated with an ischemia/reperfusion injury, with accumulation of small numbers of neutrophils and decreased glutathione in the PHA biopsies. Following reperfusion, the expression of 129 genes increased and 106 genes decreased when compared to OPENING levels (> or |
| doi_str_mv | 10.1111/j.1600-6143.2006.01254.x |
| format | Article |
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D. ; Heathcote, J. ; Edwards, A. M. ; Grant, D. ; McGilvray, I. D.</creator><creatorcontrib>Borozan, I. ; Chen, L. ; Sun, J. ; Tannis, L. ‐L. ; Guindi, M. ; Rotstein, O. D. ; Heathcote, J. ; Edwards, A. M. ; Grant, D. ; McGilvray, I. D.</creatorcontrib><description>During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well‐understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies were taken early in the donor operation (OPENING), after transection of the donor liver (PRECLAMP) and following implantation of the graft (post hepatic artery, [PHA]); these were evaluated for histology, tissue glutathione content and gene expression using a 19K‐human cDNA microarray. LDLT was associated with an ischemia/reperfusion injury, with accumulation of small numbers of neutrophils and decreased glutathione in the PHA biopsies. Following reperfusion, the expression of 129 genes increased and 106 genes decreased when compared to OPENING levels (> or <2‐fold, p < 0.01). By real‐time PCR a subset of 25 genes was verified (15 increased, 10 decreased). These genes were similarly altered in another condition of acute liver stress (the response to braindeath), but not in three chronic liver disease states (HCV, HBV and PBC). This study has identified a set of genes whose expression is altered in acute, but not chronic, liver stress, likely to play a central role in the pathogenesis of acute liver injury of liver transplantation.</description><identifier>ISSN: 1600-6135</identifier><identifier>EISSN: 1600-6143</identifier><identifier>DOI: 10.1111/j.1600-6143.2006.01254.x</identifier><identifier>PMID: 16539639</identifier><language>eng</language><publisher>Oxford UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Biological and medical sciences ; Chronic Disease ; Gene Expression ; Gene Expression Profiling ; Genes ; Hepatitis B virus ; Hepatitis C virus ; Humans ; ischemia/reperfusion ; Liver - metabolism ; Liver - surgery ; Liver Diseases - genetics ; Liver Transplantation ; Living Donors ; Male ; Medical sciences ; Middle Aged ; Oligonucleotide Array Sequence Analysis ; Reperfusion Injury - genetics ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; transplantation</subject><ispartof>American journal of transplantation, 2006-04, Vol.6 (4), p.806-824</ispartof><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4944-9f694ae459312eb721d3697a96989e6dc0f17f8b40c94c185db50ab05e23b1873</citedby><cites>FETCH-LOGICAL-c4944-9f694ae459312eb721d3697a96989e6dc0f17f8b40c94c185db50ab05e23b1873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-6143.2006.01254.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-6143.2006.01254.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17604267$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16539639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borozan, I.</creatorcontrib><creatorcontrib>Chen, L.</creatorcontrib><creatorcontrib>Sun, J.</creatorcontrib><creatorcontrib>Tannis, L. ‐L.</creatorcontrib><creatorcontrib>Guindi, M.</creatorcontrib><creatorcontrib>Rotstein, O. D.</creatorcontrib><creatorcontrib>Heathcote, J.</creatorcontrib><creatorcontrib>Edwards, A. M.</creatorcontrib><creatorcontrib>Grant, D.</creatorcontrib><creatorcontrib>McGilvray, I. D.</creatorcontrib><title>Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation</title><title>American journal of transplantation</title><addtitle>Am J Transplant</addtitle><description>During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well‐understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies were taken early in the donor operation (OPENING), after transection of the donor liver (PRECLAMP) and following implantation of the graft (post hepatic artery, [PHA]); these were evaluated for histology, tissue glutathione content and gene expression using a 19K‐human cDNA microarray. LDLT was associated with an ischemia/reperfusion injury, with accumulation of small numbers of neutrophils and decreased glutathione in the PHA biopsies. Following reperfusion, the expression of 129 genes increased and 106 genes decreased when compared to OPENING levels (> or <2‐fold, p < 0.01). By real‐time PCR a subset of 25 genes was verified (15 increased, 10 decreased). These genes were similarly altered in another condition of acute liver stress (the response to braindeath), but not in three chronic liver disease states (HCV, HBV and PBC). This study has identified a set of genes whose expression is altered in acute, but not chronic, liver stress, likely to play a central role in the pathogenesis of acute liver injury of liver transplantation.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Chronic Disease</subject><subject>Gene Expression</subject><subject>Gene Expression Profiling</subject><subject>Genes</subject><subject>Hepatitis B virus</subject><subject>Hepatitis C virus</subject><subject>Humans</subject><subject>ischemia/reperfusion</subject><subject>Liver - metabolism</subject><subject>Liver - surgery</subject><subject>Liver Diseases - genetics</subject><subject>Liver Transplantation</subject><subject>Living Donors</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Reperfusion Injury - genetics</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>transplantation</subject><issn>1600-6135</issn><issn>1600-6143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkDtPwzAQgC0E4v0XUBbYGnx-xgNDRXmqEkiUgclyXAelSpNiJ1D-PTGNYAQvd_J9dz5_CCWAU-jP-SIFgfFIAKMpwVikGAhn6XoL7f8Utn9yyvfQQQgLjEGSjOyiPRCcKkHVPnq5cbVLrtYr70Iomzp59E1RVmX9mjRFMrZd65Jp-e588tRGJJl0Phb7uxgmTd34AZh5U4dVZerWtP2kI7RTmCq44yEeoufrq9nl7Wj6cHN3OZ6OLFOMjVQhFDOOcUWBuFwSmFOhpFFCZcqJucUFyCLLGbaKWcj4POfY5Jg7QnPIJD1EZ5u5K9-8dS60elkG66p-Edd0QQspOUie_QmChIxgED2YbUDrmxC8K_TKl0vjPzVgHf3rhY5qddSso3_97V-v-9aT4Y0uX7r5b-MgvAdOB8AEa6qid2bL8MtJgRkR8VcXG-6jrNznvxfQ4_tZzOgX2Jifxg</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Borozan, I.</creator><creator>Chen, L.</creator><creator>Sun, J.</creator><creator>Tannis, L. ‐L.</creator><creator>Guindi, M.</creator><creator>Rotstein, O. D.</creator><creator>Heathcote, J.</creator><creator>Edwards, A. M.</creator><creator>Grant, D.</creator><creator>McGilvray, I. D.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200604</creationdate><title>Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation</title><author>Borozan, I. ; Chen, L. ; Sun, J. ; Tannis, L. ‐L. ; Guindi, M. ; Rotstein, O. D. ; Heathcote, J. ; Edwards, A. M. ; Grant, D. ; McGilvray, I. D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4944-9f694ae459312eb721d3697a96989e6dc0f17f8b40c94c185db50ab05e23b1873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Chronic Disease</topic><topic>Gene Expression</topic><topic>Gene Expression Profiling</topic><topic>Genes</topic><topic>Hepatitis B virus</topic><topic>Hepatitis C virus</topic><topic>Humans</topic><topic>ischemia/reperfusion</topic><topic>Liver - metabolism</topic><topic>Liver - surgery</topic><topic>Liver Diseases - genetics</topic><topic>Liver Transplantation</topic><topic>Living Donors</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Reperfusion Injury - genetics</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borozan, I.</creatorcontrib><creatorcontrib>Chen, L.</creatorcontrib><creatorcontrib>Sun, J.</creatorcontrib><creatorcontrib>Tannis, L. ‐L.</creatorcontrib><creatorcontrib>Guindi, M.</creatorcontrib><creatorcontrib>Rotstein, O. D.</creatorcontrib><creatorcontrib>Heathcote, J.</creatorcontrib><creatorcontrib>Edwards, A. M.</creatorcontrib><creatorcontrib>Grant, D.</creatorcontrib><creatorcontrib>McGilvray, I. 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M.</au><au>Grant, D.</au><au>McGilvray, I. D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation</atitle><jtitle>American journal of transplantation</jtitle><addtitle>Am J Transplant</addtitle><date>2006-04</date><risdate>2006</risdate><volume>6</volume><issue>4</issue><spage>806</spage><epage>824</epage><pages>806-824</pages><issn>1600-6135</issn><eissn>1600-6143</eissn><abstract>During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well‐understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies were taken early in the donor operation (OPENING), after transection of the donor liver (PRECLAMP) and following implantation of the graft (post hepatic artery, [PHA]); these were evaluated for histology, tissue glutathione content and gene expression using a 19K‐human cDNA microarray. LDLT was associated with an ischemia/reperfusion injury, with accumulation of small numbers of neutrophils and decreased glutathione in the PHA biopsies. Following reperfusion, the expression of 129 genes increased and 106 genes decreased when compared to OPENING levels (> or <2‐fold, p < 0.01). By real‐time PCR a subset of 25 genes was verified (15 increased, 10 decreased). These genes were similarly altered in another condition of acute liver stress (the response to braindeath), but not in three chronic liver disease states (HCV, HBV and PBC). This study has identified a set of genes whose expression is altered in acute, but not chronic, liver stress, likely to play a central role in the pathogenesis of acute liver injury of liver transplantation.</abstract><cop>Oxford UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16539639</pmid><doi>10.1111/j.1600-6143.2006.01254.x</doi><tpages>19</tpages></addata></record> |
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| subjects | Adult Biological and medical sciences Chronic Disease Gene Expression Gene Expression Profiling Genes Hepatitis B virus Hepatitis C virus Humans ischemia/reperfusion Liver - metabolism Liver - surgery Liver Diseases - genetics Liver Transplantation Living Donors Male Medical sciences Middle Aged Oligonucleotide Array Sequence Analysis Reperfusion Injury - genetics Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases transplantation |
| title | Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation |
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