Development of a quality control method for the characterization of oligonucleotides by capillary zone electrophoresis-electrospray ionization-quadrupole time of flight-mass spectrometry

A capillary zone electrophoresis‐negative electrospray ionization‐quadrupole time of flight‐mass spectrometric method was developed for the characterization of oligonucleotides after synthesis, using model compounds. The major difficulty is the adduction of metal cations to the polyanionic backbone...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Electrophoresis 2005-04, Vol.26 (7-8), p.1412-1423
Hauptverfasser:	Willems, An V., Deforce, Dieter L., Van Peteghem, Carlos H., Van Bocxlaer, Jan F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Buffers Capillary zone electrophoresis Chromatography, High Pressure Liquid Electrophoresis, Capillary - methods Electrospray ionization-mass spectrometry Hydrogen-Ion Concentration Oligonucleotides Oligonucleotides - chemistry Oligonucleotides - isolation & purification Quadrupole-time of flight Quality Control Spectrometry, Mass, Electrospray Ionization - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!