siRNA—Getting the message out
The recent observation that potent and sequence-specific gene silencing by injection of double-stranded RNA (dsRNA) has sparked the phenomenon known as “RNA interference” (RNAi) and has enabled the gene-specific knockdown of drug transport proteins and metabolizing enzymes. The application of small...
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| Veröffentlicht in: | European journal of pharmaceutical sciences 2006-04, Vol.27 (5), p.401-410 |
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| container_title | European journal of pharmaceutical sciences |
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| creator | Lee, S.H. Sinko, P.J. |
| description | The recent observation that potent and sequence-specific gene silencing by injection of double-stranded RNA (dsRNA) has sparked the phenomenon known as “RNA interference” (RNAi) and has enabled the gene-specific knockdown of drug transport proteins and metabolizing enzymes. The application of small interfering RNAs (siRNAs) is broad and the potential for use as research tools is now well established in vitro. In vivo use is still a challenge that is primarily focused on the difficulty of delivering siRNAs to target cells. The potential use of siRNAs as therapeutic agents is also exciting and holds great promise for future. For the study of drug transporter function in absorption, distribution, metabolism and excretion (ADME) and in the treatment of diseases, siRNA offers a way to gather interpretable mechanistic data—a distinct advantage over the use of “specific” chemical inhibitors. This mini review provides background information on siRNA as well as examples of the use of siRNA as applied to drug transporters. |
| doi_str_mv | 10.1016/j.ejps.2005.12.002 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0928-0987 |
| ispartof | European journal of pharmaceutical sciences, 2006-04, Vol.27 (5), p.401-410 |
| issn | 0928-0987 1879-0720 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Caco-2 Cells Digoxin - metabolism Drug transport Gene knockdown Gene silencing Gene Targeting Genetic Therapy Humans Luciferases, Renilla Neoplasms - genetics Neoplasms - metabolism Neoplasms - therapy Nervous System Diseases - genetics Nervous System Diseases - metabolism Nervous System Diseases - therapy RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism RNA, Small Interfering - therapeutic use Silencer RNA siRNA Transfection Transporters |
| title | siRNA—Getting the message out |
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