Direct separation of regio- and enantiomeric isomers of diacylglycerols by a tandem column high-performance liquid chromatography

A novel HPLC-based method for direct separation of the three isomers of mono-acid diacylglycerols (DAGs), i.e., 1,2-DAG, 2,3-DAG and 1,3-DAG, has been established. The method employs a tandem column system, in which two different columns (a conventional silica gel column and a chiral stationary phas...

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Veröffentlicht in:	Journal of Chromatography A 2005-03, Vol.1068 (2), p.243-248
Hauptverfasser:	Piyatheerawong, Weera, Iwasaki, Yugo, Yamane, Tsuneo
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Chemistry Chiral stationary phase Chromatographic methods and physical methods associated with chromatography Chromatography, High Pressure Liquid - methods Diacylglycerols Diglycerides - isolation & purification Enantiomeric resolution Ethanolysis Exact sciences and technology Fundamental and applied biological sciences. Psychology Fungal Proteins Glycerol - analogs & derivatives Glycerol - isolation & purification Isomerism Lipase - metabolism Methods. Procedures. Technologies Oleic Acids - isolation & purification Other chromatographic methods Rhizomucor - enzymology Stereoisomerism Tandem column Triglycerides - metabolism
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