Use of rhBMP-2 Activated Chitosan Films To Improve Osseointegration
Considering the design and development of biomaterials used in tissue engineering, not only is it important that they are biocompatible but also that they induce the desired cellular response for tissue regeneration. Chitosan, a biocompatible and bioresorbable polymer of N-acetylglucosamine and gluc...
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| Veröffentlicht in: | Biomacromolecules 2006-03, Vol.7 (3), p.792-798 |
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| creator | López-Lacomba, J. L García-Cantalejo, J. M Sanz Casado, J. V Abarrategi, A Correas Magaña, V Ramos, V |
| description | Considering the design and development of biomaterials used in tissue engineering, not only is it important that they are biocompatible but also that they induce the desired cellular response for tissue regeneration. Chitosan, a biocompatible and bioresorbable polymer of N-acetylglucosamine and glucosamine is used in our work combined with recombinant human BMP-2 (rhBMP-2), a potentially useful activation factor for bone repair. In this way, we try to combine the biological and filmogenic properties of this biopolymer with the osseoinductive ability of the rhBMP-2. Results showed that the chitosan films employed, without and with rhBMP-2 activation, are able to support cellular growth and proliferation on them and that only the rhBMP-2 activated ones are able to differentiate from a myoblastic mouse cell line (C2C12) toward osteoblastic phenotype. Osseoinduction properties of rhBMP-2 activated films persist for a long storage time. The in vivo experiments performed confirm the expectative created by the in vitro results obtained and are an indication that rhBMP-2 activated chitosan films could be a very attractive biomaterial for the enhancement of osseointegration of surgical prostheses and implants and for the purpose of tissue engineering bone regeneration. |
| doi_str_mv | 10.1021/bm050859e |
| format | Article |
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L ; García-Cantalejo, J. M ; Sanz Casado, J. V ; Abarrategi, A ; Correas Magaña, V ; Ramos, V</creator><creatorcontrib>López-Lacomba, J. L ; García-Cantalejo, J. M ; Sanz Casado, J. V ; Abarrategi, A ; Correas Magaña, V ; Ramos, V</creatorcontrib><description>Considering the design and development of biomaterials used in tissue engineering, not only is it important that they are biocompatible but also that they induce the desired cellular response for tissue regeneration. Chitosan, a biocompatible and bioresorbable polymer of N-acetylglucosamine and glucosamine is used in our work combined with recombinant human BMP-2 (rhBMP-2), a potentially useful activation factor for bone repair. In this way, we try to combine the biological and filmogenic properties of this biopolymer with the osseoinductive ability of the rhBMP-2. Results showed that the chitosan films employed, without and with rhBMP-2 activation, are able to support cellular growth and proliferation on them and that only the rhBMP-2 activated ones are able to differentiate from a myoblastic mouse cell line (C2C12) toward osteoblastic phenotype. Osseoinduction properties of rhBMP-2 activated films persist for a long storage time. The in vivo experiments performed confirm the expectative created by the in vitro results obtained and are an indication that rhBMP-2 activated chitosan films could be a very attractive biomaterial for the enhancement of osseointegration of surgical prostheses and implants and for the purpose of tissue engineering bone regeneration.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm050859e</identifier><identifier>PMID: 16529416</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Applied sciences ; Biocompatible Materials - chemistry ; Biological and medical sciences ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins - pharmacology ; Cell Culture Techniques - instrumentation ; Chitin - chemistry ; Chitosan - chemistry ; Crustacea ; Exact sciences and technology ; Fluoresceins - chemistry ; Humans ; Male ; Medical sciences ; Mice ; Molecular Weight ; Natural polymers ; Osseointegration ; Physicochemistry of polymers ; Rabbits ; Recombinant Proteins - pharmacology ; Starch and polysaccharides ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. 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Results showed that the chitosan films employed, without and with rhBMP-2 activation, are able to support cellular growth and proliferation on them and that only the rhBMP-2 activated ones are able to differentiate from a myoblastic mouse cell line (C2C12) toward osteoblastic phenotype. Osseoinduction properties of rhBMP-2 activated films persist for a long storage time. 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| subjects | Animals Applied sciences Biocompatible Materials - chemistry Biological and medical sciences Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - pharmacology Cell Culture Techniques - instrumentation Chitin - chemistry Chitosan - chemistry Crustacea Exact sciences and technology Fluoresceins - chemistry Humans Male Medical sciences Mice Molecular Weight Natural polymers Osseointegration Physicochemistry of polymers Rabbits Recombinant Proteins - pharmacology Starch and polysaccharides Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Transforming Growth Factor beta - pharmacology |
| title | Use of rhBMP-2 Activated Chitosan Films To Improve Osseointegration |
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