Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation
O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secret...
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| Veröffentlicht in: | Acta biochimica polonica 2005-01, Vol.52 (1), p.195-206 |
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| container_title | Acta biochimica polonica |
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| creator | Perlińska-Lenart, Urszula Kurzatkowski, Wiesław Janas, Piotr Kopińska, Agnieszka Palamarczyk, Grazyna Kruszewska, Joanna S |
| description | O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion. |
| doi_str_mv | 10.18388/abp.2005_3507 |
| format | Article |
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Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. 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Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. 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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Aspergillus nidulans - enzymology Aspergillus nidulans - genetics Aspergillus nidulans - metabolism Aspergillus nidulans - ultrastructure Base Sequence DNA Primers Fungal Proteins - biosynthesis Fungal Proteins - metabolism Glycosylation Microscopy, Electron, Transmission Mutation |
| title | Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation |
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