CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells
The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation in vitro , hES cells can acquire cytogenetic abnormalities 1 , 2 , 3 as well as submicroscopic genetic lesio...
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| Veröffentlicht in: | Nature biotechnology 2006-03, Vol.24 (3), p.351-357 |
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| creator | Herszfeld, Daniella Wolvetang, Ernst Langton-Bunker, Emma Chung, Tung-Liang Filipczyk, Adam A Houssami, Souheir Jamshidi, Pegah Koh, Karen Laslett, Andrew L Michalska, Anna Nguyen, Linh Reubinoff, Benjamin E Tellis, Irene Auerbach, Jonathan M Ording, Carol J Looijenga, Leendert H J Pera, Martin F |
| description | The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation
in vitro
, hES cells can acquire cytogenetic abnormalities
1
,
2
,
3
as well as submicroscopic genetic lesions, such as small amplifications or deletions
4
. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis. |
| doi_str_mv | 10.1038/nbt1197 |
| format | Article |
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in vitro
, hES cells can acquire cytogenetic abnormalities
1
,
2
,
3
as well as submicroscopic genetic lesions, such as small amplifications or deletions
4
. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt1197</identifier><identifier>PMID: 16501577</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Bioinformatics ; Biological and medical sciences ; Biomarkers - analysis ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Carcinoma, Embryonal - metabolism ; Carcinoma, Embryonal - pathology ; Cell Culture Techniques ; Cell Differentiation ; Cell Line, Transformed ; Cell Survival ; Cell Transformation, Neoplastic ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; Genetic abnormalities ; Genetics ; Humans ; Immunohistochemistry ; Karyotyping ; Ki-1 Antigen - metabolism ; Lesions ; letter ; Life Sciences ; Medical research ; Oncology ; Pluripotent Stem Cells - cytology ; Pluripotent Stem Cells - metabolism ; Quality control ; Skin & tissue grafts ; Stem cells</subject><ispartof>Nature biotechnology, 2006-03, Vol.24 (3), p.351-357</ispartof><rights>Springer Nature America, Inc. 2006</rights><rights>2006 INIST-CNRS</rights><rights>COPYRIGHT 2006 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Mar 2006</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c604t-6eb38f63db92abb966569f6a32957a4af00ce69b3cb177f3d2d21335eb5554a93</citedby><cites>FETCH-LOGICAL-c604t-6eb38f63db92abb966569f6a32957a4af00ce69b3cb177f3d2d21335eb5554a93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt1197$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt1197$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17585401$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16501577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herszfeld, Daniella</creatorcontrib><creatorcontrib>Wolvetang, Ernst</creatorcontrib><creatorcontrib>Langton-Bunker, Emma</creatorcontrib><creatorcontrib>Chung, Tung-Liang</creatorcontrib><creatorcontrib>Filipczyk, Adam A</creatorcontrib><creatorcontrib>Houssami, Souheir</creatorcontrib><creatorcontrib>Jamshidi, Pegah</creatorcontrib><creatorcontrib>Koh, Karen</creatorcontrib><creatorcontrib>Laslett, Andrew L</creatorcontrib><creatorcontrib>Michalska, Anna</creatorcontrib><creatorcontrib>Nguyen, Linh</creatorcontrib><creatorcontrib>Reubinoff, Benjamin E</creatorcontrib><creatorcontrib>Tellis, Irene</creatorcontrib><creatorcontrib>Auerbach, Jonathan M</creatorcontrib><creatorcontrib>Ording, Carol J</creatorcontrib><creatorcontrib>Looijenga, Leendert H J</creatorcontrib><creatorcontrib>Pera, Martin F</creatorcontrib><title>CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Nat Biotechnol</addtitle><description>The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation
in vitro
, hES cells can acquire cytogenetic abnormalities
1
,
2
,
3
as well as submicroscopic genetic lesions, such as small amplifications or deletions
4
. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.</description><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Carcinoma, Embryonal - metabolism</subject><subject>Carcinoma, Embryonal - pathology</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Cell Line, Transformed</subject><subject>Cell Survival</subject><subject>Cell Transformation, Neoplastic</subject><subject>Cells, Cultured</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic abnormalities</subject><subject>Genetics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Karyotyping</subject><subject>Ki-1 Antigen - metabolism</subject><subject>Lesions</subject><subject>letter</subject><subject>Life Sciences</subject><subject>Medical research</subject><subject>Oncology</subject><subject>Pluripotent Stem Cells - cytology</subject><subject>Pluripotent Stem Cells - metabolism</subject><subject>Quality control</subject><subject>Skin & tissue grafts</subject><subject>Stem cells</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0klr3DAUAGBTWpo0Lf0HRRS6HZxqsZ5GxzDdAoFAl1yNJEtTp7Y8leTQ_vu-YQxDckmlg8TTp_1V1XNGTxkVq_fRFsa0elAdM9lAzUDDQ-zTlaopk3BUPcn5mlIKDcDj6oiBxLBSx9XV-oOgpM_EkDynm_7GDCQYV6ZETOwwavtpNOmXTyRgrCQTM3ZG35Gf82gi2Q5z6rdT8bGQXPxInB-G_LR6FMyQ_bOlPal-fPr4ff2lvrj8fL4-u6gd0KbU4K1YBRCd1dxYqwEk6ABGcC2VaUyg1HnQVjjLlAqi4x1nQkhvpZSN0eKker1fd5um37PPpR37vDuBiX6acwtKca2A3gu5ZgyZuBcyRTUwDghf3oHX05wi3rblWLSgbHe-0z3amMG3fQwTvqDD2vmxd1P0ocf4GcPdVxwkxwnvbk1AU_yfsjFzzu35t6__by-vbts3e-vSlHPyod2mHj_2b8tou0uhdkkhlC-We80Wv_nglpxB8GoBJjszBEwJ1-eDU3IlG8rQvd27jENx49Phge7u-Q_lz9d7</recordid><startdate>20060301</startdate><enddate>20060301</enddate><creator>Herszfeld, Daniella</creator><creator>Wolvetang, Ernst</creator><creator>Langton-Bunker, Emma</creator><creator>Chung, Tung-Liang</creator><creator>Filipczyk, Adam A</creator><creator>Houssami, Souheir</creator><creator>Jamshidi, Pegah</creator><creator>Koh, Karen</creator><creator>Laslett, Andrew L</creator><creator>Michalska, Anna</creator><creator>Nguyen, Linh</creator><creator>Reubinoff, Benjamin E</creator><creator>Tellis, Irene</creator><creator>Auerbach, Jonathan M</creator><creator>Ording, Carol J</creator><creator>Looijenga, Leendert H J</creator><creator>Pera, Martin F</creator><general>Nature Publishing Group US</general><general>Nature</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>M7S</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7TB</scope><scope>7X8</scope></search><sort><creationdate>20060301</creationdate><title>CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells</title><author>Herszfeld, Daniella ; Wolvetang, Ernst ; Langton-Bunker, Emma ; Chung, Tung-Liang ; Filipczyk, Adam A ; Houssami, Souheir ; Jamshidi, Pegah ; Koh, Karen ; Laslett, Andrew L ; Michalska, Anna ; Nguyen, Linh ; Reubinoff, Benjamin E ; Tellis, Irene ; Auerbach, Jonathan M ; Ording, Carol J ; Looijenga, Leendert H J ; Pera, Martin F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c604t-6eb38f63db92abb966569f6a32957a4af00ce69b3cb177f3d2d21335eb5554a93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Carcinoma, Embryonal - metabolism</topic><topic>Carcinoma, Embryonal - pathology</topic><topic>Cell Culture Techniques</topic><topic>Cell Differentiation</topic><topic>Cell Line, Transformed</topic><topic>Cell Survival</topic><topic>Cell Transformation, Neoplastic</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. 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Biotechnol</addtitle><date>2006-03-01</date><risdate>2006</risdate><volume>24</volume><issue>3</issue><spage>351</spage><epage>357</epage><pages>351-357</pages><issn>1087-0156</issn><eissn>1546-1696</eissn><coden>NABIF9</coden><abstract>The application of human embryonic stem (hES) cells in regenerative medicine will require rigorous quality control measures to ensure the safety of hES cell–derived grafts. During propagation
in vitro
, hES cells can acquire cytogenetic abnormalities
1
,
2
,
3
as well as submicroscopic genetic lesions, such as small amplifications or deletions
4
. Many of the genetic abnormalities that arise in hES cell cultures are also implicated in human cancer development. The causes of genetic instability of hES cells in culture are poorly understood, and commonly used cytogenetic methods for detection of abnormal cells are capable only of low-throughput analysis on small numbers of cells. The identification of biomarkers of genetic instability in hES cells would greatly facilitate the development of culture methods that preserve genomic integrity. Here we show that CD30, a member of the tumor necrosis factor receptor superfamily, is expressed on transformed but not normal hES cells, and that CD30 expression protects hES cells against apoptosis.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>16501577</pmid><doi>10.1038/nbt1197</doi><tpages>7</tpages></addata></record> |
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| ispartof | Nature biotechnology, 2006-03, Vol.24 (3), p.351-357 |
| issn | 1087-0156 1546-1696 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67729760 |
| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Bioinformatics Biological and medical sciences Biomarkers - analysis Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Carcinoma, Embryonal - metabolism Carcinoma, Embryonal - pathology Cell Culture Techniques Cell Differentiation Cell Line, Transformed Cell Survival Cell Transformation, Neoplastic Cells, Cultured Fundamental and applied biological sciences. Psychology Genetic abnormalities Genetics Humans Immunohistochemistry Karyotyping Ki-1 Antigen - metabolism Lesions letter Life Sciences Medical research Oncology Pluripotent Stem Cells - cytology Pluripotent Stem Cells - metabolism Quality control Skin & tissue grafts Stem cells |
| title | CD30 is a survival factor and a biomarker for transformed human pluripotent stem cells |
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