Two-dimensional HPLC on-line analysis of phosphopeptides using titania and monolithic columns

Conventional and comprehensive two-dimensional (2D) HPLC systems using the combination of titania and monolithic columns were established for the on-line analysis of phosphopeptides. Compared with immobilized metal affinity chromatography of a general method for the analysis of phosphopeptides, the...

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Veröffentlicht in:	Analytical biochemistry 2006-03, Vol.350 (2), p.292-297
Hauptverfasser:	Hata, Koji, Morisaka, Hironobu, Hara, Keisuke, Mima, Joji, Yumoto, Noboru, Tatsu, Yoshiro, Furuno, Masahiro, Ishizuka, Norio, Ueda, Mitsuyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	2D HPLC Caseins - metabolism Chromatography, High Pressure Liquid - instrumentation Chromatography, High Pressure Liquid - methods Monolithic silica column Online Systems - instrumentation Phosphopeptides - analysis Phosphoproteome Silicon Dioxide Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Titania column Titanium Trypsin - metabolism
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