Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization
Isosorbide 5-mononitrate (5-ISMN) is an organic nitrate widely used for its vasodilating properties in the treatment of angina pectoris. In the present study, an efficient, sensitive, robust method was developed for the determination and quantification of isosorbide 5-mononitrate, in human plasma, b...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-03, Vol.832 (2), p.302-306 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Silva, Lara C. Oliveira, Lina S.O.B. Mendes, Gustavo D. Garcia, Gabriel Pereira, Alberto dos Santos De Nucci, Gilberto |
| description | Isosorbide 5-mononitrate (5-ISMN) is an organic nitrate widely used for its vasodilating properties in the treatment of angina pectoris. In the present study, an efficient, sensitive, robust method was developed for the determination and quantification of isosorbide 5-mononitrate, in human plasma, by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), using photospray ionization. Isosorbide 5-mononitrate was extracted from 0.5
mL human plasma by liquid–liquid extraction (LLE). The method had a chromatographic run of 2.0
min using a C
8 analytical column (100
mm
×
2.1
mm i.d.) and the linear calibration curve over the range was linear from 20 to 2000
ng
mL
−1 (
r
2
>
0.995). The between-run precision, based on the relative standard deviation replicate quality controls, was 7.9% (60
ng
mL
−1), 5.2% (300
ng
mL
−1) and 7.0% (1800
ng
mL
−1). The between-run accuracy was 94.9%, 94.1% and 88.8% for the above-mentioned concentrations, respectively. The method herein described was employed in a bioequivalence study of two tablet formulations of isosorbide 5-mononitrate 40
mg. |
| doi_str_mv | 10.1016/j.jchromb.2005.12.027 |
| format | Article |
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mL human plasma by liquid–liquid extraction (LLE). The method had a chromatographic run of 2.0
min using a C
8 analytical column (100
mm
×
2.1
mm i.d.) and the linear calibration curve over the range was linear from 20 to 2000
ng
mL
−1 (
r
2
>
0.995). The between-run precision, based on the relative standard deviation replicate quality controls, was 7.9% (60
ng
mL
−1), 5.2% (300
ng
mL
−1) and 7.0% (1800
ng
mL
−1). The between-run accuracy was 94.9%, 94.1% and 88.8% for the above-mentioned concentrations, respectively. The method herein described was employed in a bioequivalence study of two tablet formulations of isosorbide 5-mononitrate 40
mg.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2005.12.027</identifier><identifier>PMID: 16464650</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Atmospheric Pressure ; Biological and medical sciences ; Chromatography, Liquid - methods ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Human plasma ; Humans ; Isosorbide 5-mononitrate ; Isosorbide Dinitrate - analogs & derivatives ; Isosorbide Dinitrate - blood ; Mass Spectrometry - methods ; Medical sciences ; Pharmacology. Drug treatments ; Photochemistry ; Photospray ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Therapeutic Equivalency ; Vasodilator Agents - blood</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-03, Vol.832 (2), p.302-306</ispartof><rights>2006 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c488t-d6db5c05ebb56c03b3989cf84dc27736a6180b15fc7e9276efa1ff83964c3f8c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2005.12.027$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17572913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16464650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Silva, Lara C.</creatorcontrib><creatorcontrib>Oliveira, Lina S.O.B.</creatorcontrib><creatorcontrib>Mendes, Gustavo D.</creatorcontrib><creatorcontrib>Garcia, Gabriel</creatorcontrib><creatorcontrib>Pereira, Alberto dos Santos</creatorcontrib><creatorcontrib>De Nucci, Gilberto</creatorcontrib><title>Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Isosorbide 5-mononitrate (5-ISMN) is an organic nitrate widely used for its vasodilating properties in the treatment of angina pectoris. In the present study, an efficient, sensitive, robust method was developed for the determination and quantification of isosorbide 5-mononitrate, in human plasma, by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), using photospray ionization. Isosorbide 5-mononitrate was extracted from 0.5
mL human plasma by liquid–liquid extraction (LLE). The method had a chromatographic run of 2.0
min using a C
8 analytical column (100
mm
×
2.1
mm i.d.) and the linear calibration curve over the range was linear from 20 to 2000
ng
mL
−1 (
r
2
>
0.995). The between-run precision, based on the relative standard deviation replicate quality controls, was 7.9% (60
ng
mL
−1), 5.2% (300
ng
mL
−1) and 7.0% (1800
ng
mL
−1). The between-run accuracy was 94.9%, 94.1% and 88.8% for the above-mentioned concentrations, respectively. The method herein described was employed in a bioequivalence study of two tablet formulations of isosorbide 5-mononitrate 40
mg.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Atmospheric Pressure</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Human plasma</subject><subject>Humans</subject><subject>Isosorbide 5-mononitrate</subject><subject>Isosorbide Dinitrate - analogs & derivatives</subject><subject>Isosorbide Dinitrate - blood</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Photochemistry</subject><subject>Photospray</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Therapeutic Equivalency</subject><subject>Vasodilator Agents - blood</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2K1TAUx4sozjj6CEo2umvNx23TrkQGv2BABAV3IU1Pprk0SScnFa4r38GVr-eTmJl7YZaSxQnkd07-nF9VPWe0YZR1r_fN3swp-rHhlLYN4w3l8kF1znopaiG77w_LvZW0plzws-oJ4p5SJqkUj6sz1u3Kael59efLpkN21hmdXQwkWuIwYkyjm4C0tY8hBpeTzkBcIPPmdSDrotFrMh7I4m42N5G7JDrH66TX-fD31--swwSeeI1IcAWTyzvkdCAbunBNdPYR1xmSM2RNgLglIOsccywZ3M-7KE-rR1YvCM9O9aL69v7d18uP9dXnD58u317VZtf3uZ66aWwNbWEc285QMYqhH4ztd5PhUopOd6ynI2utkTBw2YHVzNpeDN3OCNsbcVG9Os5dU7zZALPyDg0siw4QN1SdlLxnw1DA9giaFBETWLUm53U6KEbVrRO1Vycn6taJYlwVJ6XvxemDbfQw3XedJBTg5QnQaPRikw7G4T0nW8kHJgr35shBWccPB0mhcRAMTC6VFaspuv9E-QfTCbQg</recordid><startdate>20060307</startdate><enddate>20060307</enddate><creator>Silva, Lara C.</creator><creator>Oliveira, Lina S.O.B.</creator><creator>Mendes, Gustavo D.</creator><creator>Garcia, Gabriel</creator><creator>Pereira, Alberto dos Santos</creator><creator>De Nucci, Gilberto</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060307</creationdate><title>Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization</title><author>Silva, Lara C. ; Oliveira, Lina S.O.B. ; Mendes, Gustavo D. ; Garcia, Gabriel ; Pereira, Alberto dos Santos ; De Nucci, Gilberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c488t-d6db5c05ebb56c03b3989cf84dc27736a6180b15fc7e9276efa1ff83964c3f8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Atmospheric Pressure</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Human plasma</topic><topic>Humans</topic><topic>Isosorbide 5-mononitrate</topic><topic>Isosorbide Dinitrate - analogs & derivatives</topic><topic>Isosorbide Dinitrate - blood</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Photochemistry</topic><topic>Photospray</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Therapeutic Equivalency</topic><topic>Vasodilator Agents - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Lara C.</creatorcontrib><creatorcontrib>Oliveira, Lina S.O.B.</creatorcontrib><creatorcontrib>Mendes, Gustavo D.</creatorcontrib><creatorcontrib>Garcia, Gabriel</creatorcontrib><creatorcontrib>Pereira, Alberto dos Santos</creatorcontrib><creatorcontrib>De Nucci, Gilberto</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Lara C.</au><au>Oliveira, Lina S.O.B.</au><au>Mendes, Gustavo D.</au><au>Garcia, Gabriel</au><au>Pereira, Alberto dos Santos</au><au>De Nucci, Gilberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2006-03-07</date><risdate>2006</risdate><volume>832</volume><issue>2</issue><spage>302</spage><epage>306</epage><pages>302-306</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Isosorbide 5-mononitrate (5-ISMN) is an organic nitrate widely used for its vasodilating properties in the treatment of angina pectoris. In the present study, an efficient, sensitive, robust method was developed for the determination and quantification of isosorbide 5-mononitrate, in human plasma, by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS), using photospray ionization. Isosorbide 5-mononitrate was extracted from 0.5
mL human plasma by liquid–liquid extraction (LLE). The method had a chromatographic run of 2.0
min using a C
8 analytical column (100
mm
×
2.1
mm i.d.) and the linear calibration curve over the range was linear from 20 to 2000
ng
mL
−1 (
r
2
>
0.995). The between-run precision, based on the relative standard deviation replicate quality controls, was 7.9% (60
ng
mL
−1), 5.2% (300
ng
mL
−1) and 7.0% (1800
ng
mL
−1). The between-run accuracy was 94.9%, 94.1% and 88.8% for the above-mentioned concentrations, respectively. The method herein described was employed in a bioequivalence study of two tablet formulations of isosorbide 5-mononitrate 40
mg.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>16464650</pmid><doi>10.1016/j.jchromb.2005.12.027</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2006-03, Vol.832 (2), p.302-306 |
| issn | 1570-0232 1873-376X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67728199 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Analytical, structural and metabolic biochemistry Atmospheric Pressure Biological and medical sciences Chromatography, Liquid - methods Fundamental and applied biological sciences. Psychology General pharmacology Human plasma Humans Isosorbide 5-mononitrate Isosorbide Dinitrate - analogs & derivatives Isosorbide Dinitrate - blood Mass Spectrometry - methods Medical sciences Pharmacology. Drug treatments Photochemistry Photospray Reference Standards Reproducibility of Results Sensitivity and Specificity Therapeutic Equivalency Vasodilator Agents - blood |
| title | Quantification of isosorbide 5-mononitrate in human plasma by liquid chromatography–tandem mass spectrometry using atmospheric pressure photoionization |
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