Toward the complete membrane proteome: high coverage of integral membrane proteins through transmembrane peptide detection

To attain a comprehensive membrane proteome of two strains of Corynebacterium glutamicum (l-lysine-producing and the characterized model strains), both sample pretreatment and analysis methods were optimized. Isolated bacterial membranes were digested with trypsin/cyanogen bromide or trypsin/chymotr...

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Veröffentlicht in:	Molecular & cellular proteomics 2006-03, Vol.5 (3), p.444-453
Hauptverfasser:	Fischer, Frank, Wolters, Dirk, Rögner, Matthias, Poetsch, Ansgar
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - analysis Bacterial Proteins - chemistry Cell Membrane - chemistry Chemical Fractionation Corynebacterium glutamicum Corynebacterium glutamicum - chemistry Corynebacterium glutamicum - classification Electrophoresis, Gel, Two-Dimensional Membrane Proteins - analysis Membrane Proteins - chemistry Peptides - analysis Peptides - chemistry Protein Structure, Secondary Proteome - analysis Proteome - chemistry
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