Retrieval of nearly complete 16S rRNA gene sequences from environmental DNA following 16S rRNA-based community fingerprinting

Summary 16S rRNA‐based fingerprinting techniques allow rapid analyses of overall bacterial community structure but suffer from a lack of phylogenetic information hitherto retrievable from the short 16S rRNA gene sequences obtained from excised bands. An approach is presented that allows nearly compl...

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Veröffentlicht in:	Environmental microbiology 2005-05, Vol.7 (5), p.670-675
Hauptverfasser:	Höfle, Manfred G., Flavier, Sébastien, Christen, Richard, Bötel, Julia, Labrenz, Matthias, Brettar, Ingrid
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA Fingerprinting - methods DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics Epsilonproteobacteria - genetics Oceans and Seas Polymorphism, Single-Stranded Conformational Reverse Transcriptase Polymerase Chain Reaction RNA, Bacterial - chemistry RNA, Bacterial - genetics RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Thiomicrospira denitrificans Water Microbiology
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