Comparative imaging of a bacterial surface‐located GFP fusion protein by epifluorescence and scanning near‐field optical microscopy

Summary IcsA is an autotransporter protein that plays a role in the virulence of Shigella bacteria. We have examined the cellular localization of a fusion of an IcsA fragment to the green fluorescent protein (GFP) expressed in Escherichia coli using a dual epifluorescence and scanning near‐field opt...

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Veröffentlicht in:	Journal of microscopy (Oxford) 2005-04, Vol.218 (1), p.46-51
Hauptverfasser:	GUNNING, A. P., BONGAERTS, R. J. M., KIRBY, A. R., HINTON, J. C. D., MORRIS, V. J.
Format:	Artikel
Sprache:	eng
Schlagworte:	AFM Bacterial Proteins - genetics Bacterial Proteins - metabolism DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism fluorescence Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism IcsA Microscopy, Atomic Force Microscopy, Fluorescence - methods Microscopy, Scanning Probe - methods Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Shigella Shigella - genetics Transcription Factors - genetics Transcription Factors - metabolism
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