Transmission and detection of Flavobacterium columnare in channel catfish Ictalurus punctatus

A specific and rapid PCR detection method for Flavobacterium columnare based on the 16S-23S rDNA intergenic spacer region (ISR) of the ribosomal RNA operon has been developed. The ISR of 30 F. columnare strains and other Flavobacterium species was amplified using universal primers and sequenced. Onc...

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Veröffentlicht in:	Diseases of aquatic organisms 2005-02, Vol.63 (2-3), p.129-138
Hauptverfasser:	WELKER, Thomas L, SHOEMAKER, Craig A, ARIAS, Covadonga R, KLESIUS, Phillip H
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance Animal viral diseases Animals Aquaculture Base Sequence Biological and medical sciences DNA Primers DNA, Ribosomal Spacer - genetics Evaluation Studies as Topic Fish Diseases - diagnosis Fish Diseases - microbiology Fish Diseases - transmission Flavobacteriaceae Infections - diagnosis Flavobacteriaceae Infections - transmission Flavobacteriaceae Infections - veterinary Flavobacterium - genetics Freshwater Ictaluridae Infectious diseases Medical sciences Molecular Sequence Data Polymerase Chain Reaction - methods Polymerase Chain Reaction - veterinary Sensitivity and Specificity Sequence Analysis, DNA - veterinary Species Specificity Viral diseases
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